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Efficient production of mannan-degrading enzymes by the basidiomyceteSclerotium rolfsii

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Abstract

Sclerotium rolfsii CBS 191.62 was cultivated on a number of carbon (C) sources, including mono- and disaccharides, as well as on polysaccharides, to study the formation of different mannan-degrading enzyme activities. Highest levels of mannanase activity were obtained when α-cellulose-based media were used for growth, but formation of mannanase could not be enhanced by employing galactomannan as the only carbon source. Although both xylanase and cellulase formation was almost completely repressed whenS. rolfsii was grown on more readily metabolizable carbohydrates, including glucose or mannose, considerable amounts of mannanase activity were secreted under these growth conditions. Enhanced mannanase production only commenced when glucose was depleted in the medium. The maximal mannanase activity of 240 IU/mL obtained in a laboratory fermentation is remarkable. Mannanase activity formed under these derepressed conditions could be mainly attributed to one major, acidic mannanase isoenzyme with a pI value of 2.75.

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Authors and Affiliations

  1. Abteilung Biochemische Technologie, Institut für Lebensmitteltechnologie, Universität für Bodenkultur BOKU (University of Agricultural Sciences Vienna), Muthgasse 18, A-l190, Wien, Austria

    Alois Sachslehner, Dietmar Haltrich, Bernd Nidetzky & Klaus D. Kulbe

  2. Institut für Mikrobiologie, Technische Universität Graz, Petersgasse 12, A-8010, Graz, Austria

    Georg Gübitz

Authors
  1. Alois Sachslehner
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  2. Dietmar Haltrich
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  3. Georg Gübitz
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  4. Bernd Nidetzky
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  5. Klaus D. Kulbe
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Sachslehner, A., Haltrich, D., Gübitz, G. et al. Efficient production of mannan-degrading enzymes by the basidiomyceteSclerotium rolfsii . Appl Biochem Biotechnol 70, 939–953 (1998). https://doi.org/10.1007/BF02920205

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