Summary
This paper describes the immunopathologic findings in acute malaria-associated glomerulonephritis in the rat. Young Sprague-Dawley rats were infected withPlasmodium berghei berghei. The subsequent parasitemia and elevation of circulating Clq-reactive immune complexes were transient while the appearance of anti-plasmodial antibody in the serum was persistent. Sequential examination of renal tissue and urine revealed the following glomerular alterations: (a) granular, predominantly mesangial deposits of IgG, IgM, and C 3, (b) electron dense deposits in the mesangial matrix, (c) glomerular deposition of plasmodial antigen(s) and of anti-plasmodial antibody as demonstrated by acid elution studies, (d) hypercellularity of the glomerular tufts and (e) increased urinary excretion of high molecular weight proteins. All renal abnormalities were transitory, disappearing within one to three months. The results indicate that this form of acute malarial glomerulonephritis in rats is mediated by immune complexes involving plasmodial antigen. The disease resembles the transient glomerular injury complicating cases ofPlasmodium falciparum infection in humans. As an easily reproducible model, rat malarial glomerulonephritis appears most suitable for further immunopathologic and functional studies of post-infectious glomerular disease.
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Allison AC, Houba V, Hendrickse RG, Petris S de, Edington GM, Adeniyi A (1969) Immunocomplexes in the nephrotic syndrome of African children. Lancet I: 1232–1238
Bhamarapravati N, Boonpucknavig S, Yacinboonruang C (1973)Glomerular changes in acute plasmodium falciparum infection. Arch Pathol 96: 289–293
Boonpucknavig S, Boonpucknavig V, Bhamarapravati N (1972) Immunopathological studies of Plasmodium berghei-infected mice. Arch Pathol 94: 322–330
Boonpucknavig S, Wongsawang V, Boonpucknavig N, Bhamarapravati (1976) Serum-soluble malarial antigens and immune complex nephritis in Plasmodium berghei berghei infected mice. J Trop Med Hyg 79: 116–119
Churg J, Grishman E(1972) Ultrastructure of immune deposits in renal glomeruli. Ann Intern Med 76: 479–486
McCluskey RT, Hall CL, Colvin RB (1978) Immune complex mediated diseases. Human Pathol 9: 71–84
Couser WG, Salant DJ (1980) In situ immune complex formation and glomerular injury Kidney Int 17: 1–13
Ehrich JHH, Voller A(1972) Studies on the kidneys of mice infected with rodent malaria. 1. Deposition of gamma-globulins in glomeruli in the early stages of the disease. Z Tropenmed Parasit 23: 147–152
Fawcett TK, Scott JE (1960) A rapid and precise method for the determination of urea. J Clin Pathol 13: 156–159
Germuth FG Jr, Rodriguez E (1973) Immunopathology of the renal glomerulus. Little Brown and Company, Boston, pp 15–43 and 113–161
Houba V (1979) Immunologic aspects of renal lesions associated with malarial. Kidney Int 16: 3–8
Kibukamusoke JW (1978) Quartan malarial nephropathy. Proc. 7th Inter. Congr. Nephrology, Montreal. S. Karger, Basel, pp 69–75
Laemmli UK (1971) Cleavage of structural proteins during the assembly of the head of bacteriophage TH. Nature 227: 680–685
Müller-Peddinghaus R, Meyer zu Schwabedissen H, Kalden JR, Trautwein G, Überschär (1980) Studies on the pathogenesis of Aleutian disease of mink. Zentralbl Vet Med B 27: 1–10
Musoke AJ, Cox HW, Williams JF (1977) Experimental infection with Plasmodium chabaudi in rats: Antigen and antibody associated with anemia and glomerulonephritis of acute infection. J Parasitol 63: 1081–1088
Parbtani A, Cameron JS (1979) Experimental nephritis associated with plasmodium infection in mice. Kidney Int 16: 53–63
Poels LG, van Nieker CC, Pennings L, Agterberg J, van Elven EH (1977) Plasmodium berghei: Formation of secondary immune complexes in hyperimmune mice. Exp Parasitol 43: 255–267
Sterzel RB, Krause P-H, Zobl H, Kuehn K (1974) Acute syphilitic nephrosis: A transient immunopathy. Clin Nephrol 2: 164–168
Sterzel RB, Zobl H, Krause P-H, Bahlmann J (1975) Das nephrotische Syndrom von Erwachsenen: Funktionelle, lichtoptische und immunohistologische Untersuchungen bei primären glomerulären Erkrankungen. Dtsch Med Wochenschr 100: 887–892
Sterzel RB, Krause P-H, Kregeler M (1976) Experimental changes of the mesangial capacity to handle glomerular immune deposits in rats. Contr Nephrol 2: 66–75
Suzuki M (1974) Plasmodium berghei: Experimental rodent model for malarial renal immunopathology. Exp Parasitol 35: 187–195
Voller A (1974) Immunopathology of malaria. Bull Wld Hlth Org 50: 177–186
Voller A, Bray RS (1962) Fluorescent antibody staining as a measure of malaria antibody. Proc Soc Exp Biol Med 110: 907–910
Ward PA, Conran PB (1969) Immunopathology of renal complications in simian malarial and human quartan malaria. Military Med 134: 1228–1236
Weichselbaum TE(1946) An accurate and rapid method for the determination of proteins in small amounts of blood or plasma. Am J Clin Pathol 10: 40–9
White RHR (1973) Quartan malarial nephrotic syndrome. Nephron 11: 147–162
Wilson CB, Dixon FJ (1976) The renal response to immunological injury. In: Brenner BM, Rector FC (eds) The kidney. WB Saunders Co, Philadelphia, London, Toronto, pp 838–940
Woodroffe AJ, Wilson CB (1977) An evalution of elution techniques in the study of immune complex glomerulonephritis. J Immunol 118: 1788–1794
Zubler RH, Lambert PH (1976) The125I-Clq-binding test for the detection of soluble immune complexes. In: Bloom BR, David JR (eds) In vitro methods in cell mediated and tumor immunity. New York, Academic Press, pp 565–572
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Supported in part by designated research funds of the Veterans Administration, Washington, D.C., USA, by the Deutsche Bundesministerium für Forschung und Technologie, Bonn, F.R. Germany (DFVLR 01 ZO 078) and by the Deutsche Forschungsgemeinschaft (Eh 67/2)
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Ehrich, J.H.H., Sterzel, R.B., Deicher, H.R.G. et al. Rat malarial glomerulonephritis. Virchows Archiv B Cell Pathol 37, 343–356 (1981). https://doi.org/10.1007/BF02892583
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DOI: https://doi.org/10.1007/BF02892583