Abstract
Dunaliella salina, a halotolerant unicellular green alga, can accumulate a large amount of β-carotene under certain environmental conditions. The isomers of β-carotene extracted fromD. salina cultured in medium with different nitrate and phosphate concentrations were analysed by HPLC with Alox-T alumina column. At least six isomers were found in different proportions depending on the culture media's nitrate and/or phosphate concentrations. Nitrate and/or phosphate deficiency was conducive to the accumulation of total cis isomers but not of all trans isomer. It is suggested that 1 mmol/L KNO3 and 0.1 mmol/L KH2 PO4 are favourable for accumulation of total cis β-carotene.
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Contribution No. 2090 from the Institute of Oceanology, Chinese Academy of Sciences.
This project was supported by the National Natural Science Foundation of China, Grant No. 38970587.
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Jian-guo, L., Chao-yuan, W., Nian-hong, C. et al. Effect of nitrate and phosphate on accumulation of β-carotene isomers inDunaliella salina . Chin. J. Ocean. Limnol. 14, 165–169 (1996). https://doi.org/10.1007/BF02850375
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DOI: https://doi.org/10.1007/BF02850375