Abstract
Experiments on plants are often carried out in growth chambers or greenhouses which necessitate the use of an artificial rooting environment, though this is seldom characterized in detail. Measurements were made to compare the rooting environment in large boxes (0.25 m3) with that in small pots (0.19, 0.55 and 1.90 dm3) in naturally lit chambers.
Diurnal temperature fluctuations of 14.6, 11.6 and 7.7°C occurred in the post compared with only 1.9°C in the boxes. Soil drying to a matric potential of-50 kPa was approximately 25 times faster in the pots. The mean heights of 2 year old Sitka spruce (Picea sitchensis (Bong.) Carr.) seedlings grown throughout their second growing season in the three sizes of pots were 38, 62 and 92% of the mean height of those grown in the boxes. Soil solution nutrient concentrations in the boxes were considerably increased by soil drying, an aspect which seems to have received little attention in experiments involving artificially imposed drought.
An alternative system of constraining the roots of individual plants within nylon fabric bags, embedded in larger volumes of soil, to facilitate harvesting of complete root systems is described. The importance of the rooting environment in determining the outcome of physiological experiments is also briefly discussed.
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References
Allen S E 1989 Chemical analysis of ecological materials. 2nd Edition. Blackwell Scientific Publications, Oxford.
Arp W J 1991 Effects of source-sink relations on photosynthetic acclimation to elevated CO2. Plant Cell Environ. 14, 869–875.
Davidson R L 1969 Effect of root/leaf temperature differentials on root/shoot ratios in some pasture grasses and clover. Ann. Bot. 33, 561–569.
Deans J D 1979 Fluctuations of the soil environment and fine root growth in a young Sitka spruce plantation. Plant and Soil 52, 195–208.
Harding S C and Sheehy J E 1980 Influence of shoot and root temperature on leaf growth, photosynthesis and nitrogen fixation of lucerne. Ann. Bot. 45, 229–233.
Idso S B and Kimball B A 1992 Seasonal fine-root biomass development of sour orange trees grown in atmospheres of ambient and elevated CO2 concentration. Plant Cell Environ. 15, 337–341.
Kaufmann M R 1975 Leaf water stress in Engelmann spruce-Influence of root and shoot environments. Plant Physiol. 56, 841–844.
King J A, Smith K A and Pyatt D G 1986 Water and oxygen regimes under conifer plantations and native vegetation on upland peaty gley soil and deep peat soils. J. Soil Sci. 37, 485–497.
McConnaughay K D M, Berntson G M and Bazzaz F A 1993 Plant responses to carbon dioxide. Nature 361, 24.
McCoy E L, Boersma L and Ekasingh M 1990 Net carbon allocation in soybean seedlings as influenced by soil water stress at two soil temperatures. Bot. Gaz. 151, 497–505.
Nambiar E K S, Bowen G D and Sands R 1979 Root regeneration and plant water status ofPinus radiata D. Don seedlings transplanted to different soil temperatures. J. Exp. Bot. 30, 1119–1131.
Running S W and Reid C P 1980 Soil temperature influences on root resistance ofPinus contorta seedlings. Plant Physiol. 65, 635–640.
Stevens P A 1981 Modification and operation of ceramic cup soil solution sampler for use in a geochemical cycling study. Bangor Occasional Paper No. 8, ITE, Bangor Research Station, UK.
Szaniawski R K 1983 Adaptation and functional balance between shoot and root activity of sunflower plants grown at different root temperatures. Ann. Bot. 51, 453–459.
Thomas R B and Strain B R 1991 Root restriction as a factor in photosynthetic acclimation of cotton seedlings grown in elevated carbon dioxide. Plant Physiol. 96, 627–634.
Vapaavuori E M, Rikala R and Ryyppo A 1992 Effects of root temperature on growth and photosynthesis in conifer seedlings during shoot elongation. Tree Physiol. 10, 217–230.
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Townend, J., Dickinson, A.L. A comparison of rooting environments in containers of different sizes. Plant Soil 175, 139–146 (1995). https://doi.org/10.1007/BF02413019
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DOI: https://doi.org/10.1007/BF02413019