Abstract
IgE-anti-IgE complexes were formed by preincubation of a human myeloma IgE with a monoclonal anti-human IgE antibody at different concentrations. Binding of IgE onto the anti-IgE inhibited the histamine release capacity of anti-IgE from basophils. The IgE cell-binding capacity was altered by the IgE/anti-IgE ratio in the preincubation buffer. Heating of the complexes gave a partial recovery of the anti-IgE degranulating capacity, suggesting that the monoclonal anti-IgE is specific for an epitope present on the Dɛ2 domain.
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Weyer, A., Demeulemester, C., Mazie, J.C. et al. Monoclonal anti-human IgE: histamine release capacity and effect onin vitro sensitization of human basophils. Agents and Actions 20, 206–209 (1987). https://doi.org/10.1007/BF02074670
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DOI: https://doi.org/10.1007/BF02074670