Summary
Mouse blastocysts were cultured in the presence of zinc and/or cupric chloride at various concentrations. Cupric ions were superior to Zn++ at inhibiting hatching of blastocysts from their zona pellucida and formation of trophoblastic outgrowths. Protein in the medium protected embryos from the toxic effects of zinc and copper.
Similar content being viewed by others
References
J. K. Campbell and C. F. Mills, Proc. Nutr. Soc.33, 15A (1974).
H. D. Ritchie, R. W. Lueke, B. V. Baltzer, E. R. Miller, D. E. Ullrey and J. A. Hoefer, J. Nutr.79, 117 (1963).
C. H. Hill and G. Matrone, Fedn Proc.29, 1474 (1970).
N. F. Sittle and C. F. Mills, Br. J. Nutr.20, 135 (1966).
N. T. Davies, Proc. Nutr. Soc.35, 293 (1974).
N. T. Davies, Proc. Nutr. Soc.38, 121 (1979).
I. Bremner and N. T. Davies, Biochem. Soc. Trans.2, 425 (1974).
D. R. Van Campen and P. U. Scaife, J. Nutr.91, 473 (1967).
D. R. Van Campen, J. Nutr.97, 104 (1969).
R. L. Brinster and P. C. Cross, Nature238, 398 (1972).
G. Naeslund, Contraception6, 281 (1972).
M. K. Holland and I. L. Pike, J. Reprod. Fert.53, 335 (1978).
F. B. Orlans, Contraception10, 543 (1974).
C. C. Chang, H. J. Tatum and F. A. Kincl, Fert. Steril.21, 274 (1970).
D. Gallaher and L. S. Hurley, J. Nutr.110, 591 (1980).
R. E. Fowler and R. G. Edwards, J. Endocr.15, 374 (1957).
L. J. Van Winkle, Biochem. biophys. Res. Commun.98, 562 (1981).
L. J. Van Winkle, J. exp. Zool.218, 239 (1981).
C. M. Woolf, ed., in: Principles of Biometry, p. 302. D. Van Nostrand, London 1968.
L. S. Hurley and R. E. Shrader, Nature254, 427 (1975).
A. I. Spindle and R. A. Pedersen, J. exp. Zool.186, 305 (1979).
Y-C. Hsu, Devl Biol.68, 453 (1979).
M. H. Sellens and M. I. Sherman, J. Embryol. exp. Morph.56, 1 (1980).
Author information
Authors and Affiliations
Additional information
Acknowledgment. The authors wish to thank Dr W. E. Farnsworth, Dr D. F. Mann, Mr H. Wasserlauf and Ms Barb Le Breton for their help in producing this manuscript. Supported by the Chicago College of Osteopathic Medicine.
Rights and permissions
About this article
Cite this article
Van Winkle, L.J., Campione, A.L. Toxic effects of Zn++ and Cu++ on mouse blastocysts in vitro. Experientia 38, 354–356 (1982). https://doi.org/10.1007/BF01949389
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01949389