Summary
Different strains ofBacteroides fragilis exhibit great differences in sensitivity towards serum from healthy volunteers. In the presence of 10% autologous serum, neutrophilic granulocytes and monocytes (macrophages) caused significant killing ofB. fragilis. The measured phagocytic and killing activity of the cells is comparable to their activity against aerobic bacteria (S. aureus). In four patients with chronic granulomatous disease of childhood, phagocytosis was normal but killing ofB. fragilis andS. aureus in granulocytes or monocytes (macrophages) was appreciably lowered. This malfunction of the cells was accompanied by a disturbance in oxidative metabolism and inadequate iodination after phagocytosis ofB. fragilis. The results suggest that granulocytes and monocytes play an important role in host defense against endogenous infections with anaerobes.
Similar content being viewed by others
Abbreviations
- NBT:
-
Nitroblautetrazolium
- B. fragilis:
-
Bacteroides fragilis
Literatur
Baehner RL (1974) Molecular basis of functional disorders of phagocytes. J Pediatr 84:317–327
Bjornson AB, Altemeier WA, Bjornson S (1976) Comparison of the in vitro bactericidal activity of human serum and leukocytes against Bacteroides fragilis and Fusobacterium mortiferum in aerobic and anaerobic environments. Infect Immun 14/3:843–847
Bøyum A (1968) Isolation of mononuclear cells and granulocytes from human blood. Scand. J Clin Lab Invest 21 (Suppl 97):77
Casciato DA, Rosenblatt JE, Goldberg LS, Bluestone R (1975) In vitro interaction of Bacteroides fragilis with polymorphonuclear leucocytes and serum factors. Infect Immun 11/2:337–342
Kaplow LS (1965) Simplified Myeloperoxydase stain using benzidine dihydrochloride. Blood 26/2:215–219
Lehrer IR, Cline MJ (1969) Interaction of Canadia albicans with human leucocytes and serum. J Bact 89:996–1004
Niethammer D (1980) Kongenitale Abwehrstörungen. In: Bachmann KD et al. (Hrsg) Pädiatrie in Praxis und Klinik II:1140–1145
Pincus S, Klebanoff S (1971) Quantitative leucocyte iodination. N Engl J Med 284:744–750
Preisig E, Hitzig WH (1971) Nitroblue-Tetrazoliumtest for the detection of chronic granulomatous disease, technical modification. Eur J clin Invest 1:409–412
Schaal KP (1983) Anaerobierinfektionen in der operativen Medizin. Immun Infekt 11:153–168
Werner H (1983) Anaerobierinfektionen in der Inneren Medizin. Immun Infekt 11:169–176
Wildfeuer A (1979) Zahnkaries und Parodontitis. Mikrobiologie und Immunologie. Ein Beitrag zur Entzündungslehre. C Hanser, München-Wien
Wildfeuer A, Krawinkel M, Lange C-E, Voigt W-H (1980) Leberabszeß bei chronischer Granulomatose des Kindesalters. Klin Pädiat 192:241–248
Wildfeuer A, Laufen H, Galle J, Pfänder K (1986) Intrazelluläre Wirkung von Josamycin. Arzneim Forsch/Drug Res 36 (I) 1:127–131
Author information
Authors and Affiliations
Additional information
Herrn Prof. Dr. A.K. Kleinschmidt zum 70. Geburtstag gewidmet
Rights and permissions
About this article
Cite this article
Wildfeuer, A., Dopfer, R., Niethammer, D. et al. Funktionen der körpereigenen Abwehr gegen Anaerobier bei Gesunden und Patienten mit chronisch-septischer Granulomatose. Klin Wochenschr 65, 113–120 (1987). https://doi.org/10.1007/BF01728602
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01728602