Summary
This paper describes the influence of human fibroblast interferon (IFN-β) on the cytotoxic activity of natural killer cells (NK) in vitro and in vivo using the blood of healthy donors and myeloma patients. IFN-β stimulates NK activity against all target cells tested in vitro in a dose-dependent way up to 250% of pretreatment values. At higher IFN concentrations, stimulation returned to baseline values. Stimulation was most pronounced in the lowest lymphocyte to target cell ratio. 1- to 2-h preincubation of effector cells with IFN was enough to achieve maximal stimulation. The effector cells of IFN-treated myeloma-patients, or patients with herpes zoster, showed a clear reduction of toxicity against all cells tested during the first infusion, as compared to the pretreatment values.
Zusammenfassung
Die zytotoxische Aktivität der natürlichen Killerzellen (NK) wurde bei gesunden Spendern und Patienten mit Plasmocytom unter dem Einfluß von humanem Fibroblasteninterferon (IFN-β) in vitro und in vivo gemessen. IFN-β steigerte die NK-Aktivität gegenüber allen getesteten Zielzellen in vitro dosisabhängig bis zu 250% des Ausgangswertes. Bei weiterer Erhöhung der IFN-Konzentration nahm die Stimulation wieder ab. Die Stimulation war bei niedriger Lymphozyten-Zielzell-Relation am deutlichsten zu sehen. Eine 1- bis 2stündige Präinkubation der Effektorzellen mit Interferon reichte aus, um die maximale Stimulation zu erreichen. Wurde der Einfluß von IFN-β auf NK-Zellen in vivo im Blut von Interferon-behandelten Patienten mit Plasmocytom gemessen, zeigte sich eine deutliche Verminderung der Toxizität gegenüber allen getesteten Zielzellen während der ersten Infusion im Vergleich mit dem Ausgangswert vor Interferon-Therapie.
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Heidemann, E., Reichmann, U., Wilms, K. et al. Effect of human fibroblast interferon on natural killer cell activity: Stimulation in vitro and inhibition in vivo. Klin Wochenschr 60, 625–629 (1982). https://doi.org/10.1007/BF01711438
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DOI: https://doi.org/10.1007/BF01711438