Summary
Following X-irradiation of exponentially growing L929 cells two major phenomena have been observed. First, there was a delay in cell division which can be ascribed to the arrest of cells in theG 2-phase (G 2-block), and, second, the cellular content of the O6-alkylguanine-DNA alkyltransferase (AGT) was markedly increased. Flow cytometrical DNA-measurements revealed that cells began to accumulate in theG 2-phase 4 h after irradiation (p.r.) irrespective of the X-ray dose, while both the fraction of cells blocked inG 2 and the time period the cells persisted inG 2 increased with the radiation dose. About 24 h past release from theG 2-block the distribution of cells in the cell cycle was similar to that of untreated control cells. In comparison with control cells the AGT content in irradiated cells (4 Gy) was highest at about 48 h p.r. (3.4-fold increase). The highest ratio of increase in AGT was, however, observed to occur between about 4 and 13 h p.r. (2.6-fold increase). As shown by flow cytometrical measurements using a BrdUrd/DNA double labeling technique, this rapid primary increase in AGT coincides very well with the entrance of cells into theG 2-phase. This indicates that the cellular AGT content in X-irradiated (parental) cells started to exceed the basal level at the beginning of theG 2-phase, but not before or during theS-phase. Once the AGT level was elevated it continued to increase for 2 to 3 cell doubling times.
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Paper given at the Workshop “Molecular Radiation Biology”. German Section of the DNA Repair Network, München-Neuherberg, 21.-23.3.1990
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Nehls, P., van Beuningen, D. & Karwowski, M. After X-irradiation a transient arrest of L929 cells inG 2-phase coincides with a rapid elevation of the level of O6-alkylguanine-DNA alkyltransferase. Radiat Environ Biophys 30, 21–31 (1991). https://doi.org/10.1007/BF01595571
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DOI: https://doi.org/10.1007/BF01595571