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Gangliosides inhibit PDGF-induced signal transduction events in U-1242 MG human glioma cells

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Abstract

In this study we investigated the responses of intracellular calcium ([Ca2+]i) and protein kinase C (PKC) to PDGF in U-1242 MG cells. PDGF-BB stimulated [3H]PDBu binding approximately 2–3 fold. This response was inhibited by preincubating the cells with an inhibitor of phospholipase C (PLC), U73122, suggesting that PLC mediates the induction of PKC translocation by PDGF. PDGF also increased the concentration of [Ca2+]i that was attenuated in a calcium-free medium. This indicates that PDGF-induced elevation of [Ca2+]i is mainly due to influx of extracellular calcium. PDGF-stimulated translocation of PKC was inhibited by the intracellular calcium buffer BAPTA/AM. All gangliosides studied except GM3 inhibited these responses with similar efficacy. Collectively, these results indicate that the signal transduction pathway initiated by PDGF leading to PKC translocation in U-1242 MG cells is intact, and this pathway is inhibited by several gangliosides.

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Authors and Affiliations

  1. Division of Neuropathology, Department of Pathology, The Ohio State University, Room 4168 Graves Hall, 333 West 10th Avenue, 43210, Columbus, Ohio

    H. E. Saqr & A. J. Yates

  2. College of Dentistry, The Ohio State University, 2214 Postle Hall, 305 W 12th Avenue, 43210, Columbus, Ohio

    J. D. Walters

  3. Department of Physiology, The Ohio State University, 400A Hamilton Hall 1645 Neil Ave, 43210, Columbus, Ohio

    Z. Guan & B. T. Stokes

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  1. H. E. Saqr
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Correspondence to A. J. Yates.

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Special issue dedicated to Dr. Leon S. Wolfe.

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Saqr, H.E., Walters, J.D., Guan, Z. et al. Gangliosides inhibit PDGF-induced signal transduction events in U-1242 MG human glioma cells. Neurochem Res 20, 1389–1395 (1995). https://doi.org/10.1007/BF00992515

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