Abstract
Autoantibodies from patients with systemic lupus erythematosus, rheumatoid arthritis, or other connective tissue disorders were probed for the presence of a cross-reactive idiotype (AM Id) originally defined on human anti-double-stranded DNA antibodies. The AM Id was distributed primarily among antibodies to double-stranded DNA, single-stranded DNA, or cardiolipin in patients with systemic lupus erythematosus and antibodies to single-stranded DNA or cardiolipin and rheumatoid factor in patients with rheumatoid arthritis, thus tending to codistribute with the predominant primary autoantibodies in both diseases. Strong associations were observed particularly between the AM Id and anti-single-stranded DNA antibodies in patients with systemic lupus erythematosus and the AM Id and anticardiolipin antibodies in patients with rheumatoid arthritis. Affinity absorption experiments with sera from individual lupus patients showed that up to 41% of the anti-single-stranded DNA antibodies were Id positive. The results indicate that the AM Id may be widely distributed among antibodies that have a potential for binding DNA.
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Theofilopoulos AN, Balderas RS, Hang LM, Dixon FJ: Monoclonal IgM rheumatoid factor derived from arthritic MRL/Mp-lpr/lpr mice. J Exp Med 158:901, 1983
Jerne NK: Towards a network theory of the immune system. Ann Immunol (Paris) 125C:373, 1974
Hahn BH, Ebling FM: Suppression of murine lupus nephritis by administration of an anti-idiotypic antibody to anti-DNA. J Immunol 132:187, 1983
Hahn BH, Ebling FM: Suppression of NZB/NZW murine nephritis by administration of a syngeneic monoclonal antibody to DNA: Possible role of anti-idiotypic antibodies. J Clin Invest 71:1728, 1983
Williams R Jr, Kunkel HG, Capra JD: Antigenic specificities related to the cold agglutinin activity of gamma M globulins. Science 161:379, 1968
Lefvert AK: Anti-idiotypic antibodies against the receptor antibodies in myasthenia gravis. Eur J Immunol 13:493, 1981
Zanetti M, Bigazzi PE: Anti-idiotypic immunity and autoimmunity. I.In vitro andin vivo effects of anti-idiotypic antibodies to spontaneously occurring autoantibodies to rat thyroglobulin.Eur J Immunol 11:187, 1981
Rauch J, Murphy E, Roths JB, Stollar BD, Schwartz RS: A high frequency idiotypic marker of anti-DNA autoantibodies in MRL-lpr/lpr mice. J Immunol 129:236, 1982
Kunkel HG, Agnello V, Joslin FG, Winchester RJ, Cooper JD: Cross-idiotypic specificity among monoclonal IgM-proteins with anti-gamma-globulin activity. J Exp Med 137:331, 1973
Harkiss GD, Hendrie F, Nuki G: Cross-reactive idiotypes in anti-DNA antibodies of SLE patients. Clin Immunol Immunopathol 39:421, 1985
Sharp GC, Irvine WS, Tan EM, Gould RG, Holman HR: Mixed connective tissue disease—an apparently distinct rheumatic disease syndrome associated with a specific antibody to an extractable nuclear antigen (ENA). Am J Med 52:148, 1972
Stokes RP, Cordwell A, Thompson RA: A simple, rapid Elisa method for the detection of DNA antibodies. J Clin Pathol 35:556, 1982
Hahn BH, Ebling FM: A public idiotypic determinant is present on spontaneously cationic IgG antibodies to DNA from mice of unrelated lupus-prone strains. J Immunol 133:3015, 1984
Mayus JL, Pisetsky DS: Expression of a highly conserved anti-DNA idiotype in normal and autoimmune mice. Clin Immunol Immunopathol 34:366, 1985
Datta SK, Stollar BD, Schwartz RS: Normal mice express idiotypes related to autoantibody idiotypes of lupus mice. Proc Natl Acad Sci USA 80:2723, 1983
Eilat D, Fischel R, Zlotnick A: A central anti-DNA idiotype in human and murine systemic lupus erythematosus. Eur J Immunol 15:368, 1985
Shoenfeld Y, Isenberg DA, Rauch J, Madaio MP, Stollar BD, Schwartz RS: Idiotypic cross-reactions of monoclonal human lupus autoantibodies. J Exp Med 158:718, 1983
Solomon G, Schiffenbauer J, Keiser HD, Diamond B: Use of monoclonal antibodies to identify shared idiotypes on human antibodies to native DNA from patients with systemic lupus erythematosus. Proc Natl Acad Sci USA 89:850, 1983
Colaco CB, Male DK: Anti-phospholipid antibodies in syphilis and a thrombotic subset of SLE: Distinct profiles of epitope specificity. Clin Exp Immunol 59:449, 1985
Harris EN, Gharavi AE, Boey AE, Patel ML, Mackworth-Young CG, Loizou S, Hughes GRV: Anticardiolipin antibodies: Detection by radioimmunoassay and association with thrombosis in systemic lupus erythematosus. Lancet 2:1211, 1983
Lafer EM, Rauch J, Andrzejewski C Jr, Mudd D, Furie BC, Furie B, Schwartz RS, Stollar BD: Polyspecific monoclonal lupus autoantibodies reactive with both polynucleotides and phospholipids. J Exp Med 153:897, 1981
Agnello V, Arbetter A, de Kasep GI, Powell R, Tan EM, Joslin F: Evidence for a subset of rheumatoid factors that cross-react with DNA-histone and have a distinct cross-idiotype. J Exp Med 151:1514, 1980
Hannestad K: Gamma-M rheumatoid factors reacting with nitrophenyl groups and denatured deoxyribonucleic acid. Ann NY Acad Sci 168:63, 1969
Rubin RL, Balderas RS, Tan EM, Dixon FJ, Theofilopoulos AN: Multiple autoantigen binding capabilities of mouse monoclonal antibodies selected for rheumatoid factor activity. J Exp Med 159:1429, 1984
Pisetsky DS, Hoch SO, Klatt CL, O'Donnell MA, Keene JD: Specificity and idiotypic analysis of a monoclonal anti-Sm antibody with anti-DNA activity. J Immunol 135:4080, 1985
Diamond B, Scharff MD: Somatic mutation of the T15 heavy chain gives rise to an antibody with autoantibody specificity. Proc Natl Acad Sci USA 81:5841, 1984
Eilat D, Hochberg M, Pomphrey J, Rudikoff S: Monoclonal antibodies to DNA and RNA from NZB/NZW F1 mice: Antigenic specificities and NH2 terminal amino acid sequences. J Immunol 133:489, 1984
Kofler R, Noonan DJ, Levy DE, Wilson MC, Moller NPH, Dixon FJ, Theofilopoulos AN: Genetic elements used for a murine lupus anti-DNA autoantibody are closely related to those for antibodies to exogenous antigens. J Exp Med 161:805, 1985
Atkinson PM, Lampman GW, Furie BC, Naperstek Y, Schwartz RS, Stollar BD, Furie B: Homology of the NH2-terminal amino acid sequences of the heavy and light chains of human monoclonal lupus autoantibodies containing the dominant 16/6 idiotype. J Clin Invest 75:1138, 1985
Naperstek Y, Duggan D, Schattner A, Madaio MP, Goni F, Frangione B, Stollar BD, Kabat EA, Schwartz RS: Immunochemical similarities between monoclonal antibacterial Waldenstrom's macroglobulins and monoclonal anti-DNA lupus autoantibodies. J Exp Med 161:1525, 1985
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Harkiss, G.D., Hendrie, F. & Nuki, G. Distribution of an anti-DNA idiotype among autoantibodies in patients with systemic lupus erythematosus (SLE) or rheumatoid arthritis (RA). J Clin Immunol 6, 292–298 (1986). https://doi.org/10.1007/BF00917329
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DOI: https://doi.org/10.1007/BF00917329