Summary
The electrophoretic karyotypes of strains of the ten species of the yeast genusSaccharomyces (sensu Vaughan-Martini & Martini 1992) were determined by the CHEF (contour-clamped homogeneous electric field) system of pulsed field gel electrophoresis. The number of bands was found to vary from 6 to 17 and the calculated molecular weights of haploid genomes ranged from 7.9 to 14.6 Mbp. The type strains ofS. exiguus and the four species of theSaccharomyces sensu stricto complex (S. bayanus, S. cerevisiae, S. paradoxus andS. pastorianus) have genomes comprised of chromosomes of all three size classes: light (< 500 kb), medium (500–1000 kb) and heavy (> 1,000 kb).Saccharomyces kluyveri DNA has only heavy bands, while the remaining species exhibit medium and heavy chromosomes. When more than one strain of each species was examined, it was seen that while the speciesS. bayanus, S. castellii, S. cerevisiae, S. kluyveri, S. paradoxus andS. pastorianus showed uniform karyotypes,S. dairensis, S. exiguus, S. servazzii andS. unisporus comprise heterogeneous taxa.
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References
Batschinskaya AA (1914) Entwicklungsgeschichte und Kultur des neuen HefepilzesSaccharomyces paradoxus. J. Microbiol. Epidémiol. Immunobiol. II: 231–247
Bernardi G, Faures M, Piperno G & Slonimski PP (1970) Mitochondrial DNAs from respiratory-sufficient and cytoplasmic respiratory-deficient mutants of yeasts. J. Mol. Biol. 48: 23–43.
Capriotti A (1966)Saccharomyces castellii n. sp. Una nuova specie di lievito isolata da un terreno della Finlandia. Ann. Fac. Agr. Univ. Sassari. 14: 3–8
Capriotti A (1967)Saccharomyces servazii n. sp. A new yeast from Finland soil. Ann. Microbiol. Enzimol. (Milano) 17: 79–84
Carle GF & Olsen MV (1985) An electrophoretic karyotype for yeast. Proc. Natl. Acad. Sci. USA 82: 3756–3760
Casey GP, **ao W & Rank GH (1988) Application of pulsed field chromosome electrophoresis in the study of chromosome XIII and the electrophoretic karyotype of industrial strains ofSaccharomyces yeasts. J. Inst. Brew. 94: 239–243
De Jonge P, De Jongh PCM, Meijers R, Steensma HY & Scheffers WA (1987) Orthogonal-field-alternation gel electrophoresis banding patterns of DNA from yeasts. Yeast 2: 193–204.
Hansen EC (1883) Undersøgelser over alkoholgjaersvampenes fysiologi og morfologi II Om askosporedannelsen hos slaegtenSaccharomyces. Medd. Carlsberg Lab. 2: 29–86
Hansen EC (1888) Recherches sur la physiologie et la morphologie des ferments alcooliques VII Action des ferments alcooliques sur les diverses espèces de sucre. C.R. Trav. Lab. Carlsberg 2: 143–167
Hansen EC (1904) Grundlinien für Systematik der Saccharomyceten. Zentralbl. Bakteriol. Parasitenk Abt II 12: 529–538
Henriques M, Sá-Nogueira I, Giménez-Juradao G & van Uden N (1991) Ribosomal DNA spacer probes for yeast identification: Studies in the genusMetschnikowia. Yeast 7: 167–172
Johnston JR & Mortimer RK (1986) Electrophoretic karyoty** of laboratory and commercial strains ofSaccharomyces and other yeasts. Int. J. Syst. Bacteriol. 36: 569–572
Johnston JR, Contopoulou CR & Mortimer RK (1988) Karyoty** of yeast strains of several genera by field inversion gel electrophoresis. Yeast 4: 191–198
Johnston JR, Curran L, Contopoulou RC & Mortimer RK (1989) Electrophoretic karyoty** of commercial brewing and distilling strains ofSaccharomyces and of other yeast. Yeast 5: 255–260
Jörgensen A (1909) Die Mikroorganismen der Gärungsindustrie. 5te Auflage, P Parey, Berlin
Kaneko Y, Mikata K & Banno I (1989) Karyoty** ofSaccharomyces exiguus by pulsed-field gel electrophoresis. IFO Res. Comm. 14: 111–117
Kurtzman CP, Smiley MJ, Johnson CJ, Wickerham LJ & Fuson GB (1980) Two new and closely related heterothallic species,Pichia amylophila andPichia mississippiensis: characterization by hybridization and deoxyribonucleic acid reassociation. Int. J. Syst. Bacteriol. 30: 208–216
Kurtzman CP, Phaff HJ & Meyer SA (1983) Nucleic acid relatedness among yeasts. In: JFT Spencer, DM Spencer & ARW Smith (Eds) Yeast Genetics Fundamental and Applied Aspects (pp. 139–166) Springer-Verlag, New York
Kurtzman CP & Robnett CJ (1991) Phylogenetic relationships among species ofSaccharomyces, Schizosaccharomyces, Debaryomyces andSchwanniomyces determined from partial ribosomal RNA sequences. Yeast 7: 61–72
Marmur J (1961) A procedure for the isolation of DNA from microorganisms. J. Mol. Biol. 3: 208–218
Meyer SA & Phaff HJ (1969) Deoxyrybonucleic acid base composition in yeasts. J. Bacteriol. 97: 52–56
Miller M, Kock JLF, Pretorius GHJ & Coetzee DJ (1989) The value of orthogonal-field-alternation gel electrophoresis and other criteria in the taxonomy of the genusPichia Hansen emend Kurtzman. Syst. Appl. Microbiol. 12: 191–202
Naganishi H (1917) Three new species of yeasts. Bot. Mag. Tokyo 31: 107–115
Naumov GI (1986) Genetic differentiation and ecology of the yeastSaccharomyces paradoxus Batschinskaia. Dokl. Akad. Nauk. SSSR 289: 213–216
Naumov GI (1987) Genetic basis for classification and identification of the ascomycetous yeast. pp. 469–475. In: The Expanding Realm of Yeast-like Fungi.Proc.Int.Symp. Perspectives of Taxonomy, Ecology and Phylogeny of Yeast and Yeast-like Fungi. (GS de Hoog, MTh Smith, ACM Weijman, eds). Amsterdam, Elsevier Science Publishers
Naumov G, Naumova ES, Lantto RA, Louis EJ & Korhola M (1992) Genetic homology betweenSaccharomyces cerevisiae and its sibling speciesS. paradoxus andS. bayanus: Electrophoretic karyotypes. Yeast 8: 599–612
Pedersen MB (1986) DNA sequence polymorphisms in the genusSaccharomyces IV Homoeologous chromosomes,S. carlsbergensis andS. uvarum. Carlsberg Res. Commun. 51: 185–202
Phaff HJ (1989) Trends in yeast systematics. Yeast 5: 341–350
Phaff HJ, Miller MW & Shifrine M (1956) The taxonomy of yeasts isolated fromDrosophila in the Yosemite region of California. Antonie van Leeuwenhoek 22: 145–161
Price CW, Fuson GB & Phaff HJ (1978) Genome comparison in yeast systematics: delimitation of species within the generaSchwanniomyces, Saccharomyces, Debaryomyces andPichia. Microbiol. Rev. 42: 161–193
Rank GH, Casey G & **ao W (1988) Gene transfer in industrialSaccharomyces yeasts. Food Biotech. 2: 01–41
Rosini G, Federici F, Vaughan AE & Martini A (1982) Systematics of the species of the yeast genusSaccharomyces associated with the fermentation industry. Europ. J. Appl. Microbiol. Biotechnol. 15: 188–193
Saccardo PA (1895) Sylloge Fungorum omnium hucusque cognitorum. Vol 11: pars III, 457
Schwartz DC & Cantor CR (1984) Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 37: 67–75
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503
Vaughan-Martini A (1989)Saccharomyces paradoxus comb nov, a newly separated species of theSaccharomyces sensu stricto complex based upon nDNA/nDNA homologies. Syst. Appl. Microbiol. 12: 179–182
Vaughan-Martini A & Kurtzman CP (1985) Deoxyribonucleic acid relatedness among species of the genusSaccharomyces sensu stricto. Int. J. Syst. Bacteriol. 35: 508–511
Vaughan-Martini A & Kurtzman CP (1988) Deoxiribonucleic acid relatedness among species ofSaccharomyces sensu lato. Mycologia 80: 241–243
Vaughan-Martini A & Martini A (1987) Three newly delimited species ofSaccharomyces sensu stricto. Antonie van Leeuwenhoek 53: 77–84
Vaughan-Martini A & Martini A (1992) A taxonomic key to the genusSaccharomyces Syst. Appl. Microbiol. 15: in press
Walt JP van der (1970)Saccharomyces emend Reess. In: J Lodder (ed) The Yeasts-a taxonomic study (pp. 555–718) North-Holland Publ. Co., Amsterdam
Yarrow D & Nakase T (1975) DNA base composition of species of the genusSaccharomyces. Antonie van Leeuwenhoek. 41: 81–88
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Vaughan-Martini, A., Martini, A. & Cardinali, G. Electrophoretic karyoty** as a taxonomic tool in the genusSaccharomyces . Antonie van Leeuwenhoek 63, 145–156 (1993). https://doi.org/10.1007/BF00872389
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DOI: https://doi.org/10.1007/BF00872389