Summary
The construction of a simple “incubatin chamber” is presented by which a homogeneous incubation of tissue sections with calculable amounts of immunoreagents is guaranteed. The incubation of large area sections, and parallel incubations of 10 slides are possible. As an example of application a case of alpha-1-antitrypsin deficiency in the liver is demonstrated.
References
Bartonicek V, Lojda Z (1963) Incubation chambers made of plastic. Acta Histochem 15:389–391
Graham RC, Karnowsky MJ (1966) The early stages of absorption of injected horse radish peroxidase in proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302
Lojda Z, Gossrau R, Schiebler TH (1976) Enzymhistochemische Methoden. Springer, Berlin Heidelberg New York
Lieberman J, Mittman C, Gordon HW (1972) Alpha-1-antitrypsin in the livers of patients with emphysema. Science 175:63–65
Palmer PE, Wolfe HJ, Dayal Y, Gang DL (1978) Immunocytochemical diagnosis of alpha-1-antitrypsin deficiency. Am J Surg Pathol 2:275–281
Sternberger L (1974) Immunocytochemistry 2nd ed. John Wiley and Sons, New York
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Ormanns, W., Pfeifer, U. A simple method for incubation of tissue sections in immunohistochemistry. Histochemistry 72, 315–319 (1981). https://doi.org/10.1007/BF00517144
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DOI: https://doi.org/10.1007/BF00517144