Abstract
The relationship between the degree of aerobiosis, xylitol production and the initial two key enzymes of d-xylose metabolism were investigated in the yeasts Pichia stipitis, Candida shehatae and C. tenuis. Anoxic conditions severely curtailed growth and retarded ethanol productivity. This, together with the inverse relationship between xylitol accumulation and aeration level, suggested a degree of redox imbalance. The ratios of NADH- to NADPH-linked xylose reductase were similar in all three yeasts and essentially independent of the degree of aerobiosis, and thus did not correlate with their differing capacities for ethanol production, xylitol accumulation or growth under the different conditions of aerobiosis. Under anoxic conditions the enzyme activity of Pichia stipitis decreased significantly, which possibly contributed to its weaker anoxic fermentation of xylose compared to C. shehatae.
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References
Baillargeon MW, Jansen MB, Gong C-S, Tsao GT (1983) Effect of oxygen uptake rate on ethanol production by a xylose-fermenting yeast mutant, Candida sp. XF217. Biotechnol Lett 5:339–344
Bergmeyer HU (ed) (1974) Methods of enzymatic analysis, 2nd edn, vol 1. Academic Press, New York London, pp 172–174
Bolen PL, Detroy RW (1985) Induction of NADPH-linked d-xylose reductase and NAD-linked xylitol dehydrogenase activities in Pachysolen tannophilus by d-xylose, l-arabinose, or d-galactose. Biotechnol Bioeng 27:302–307
Bolen PL, Bietz JA, Detroy RW (1985) Aldose reductase in the yeast Pachysolen tannophilus: purification, characterization, and N-terminal sequence. Biotechnol Bioeng Symp 15:129–148
Bruinenberg PM, Van Dijken JP, Scheffers WA (1983) An enzymatic analysis of NADPH production and consumption in Candida utilis. J Gen Microbiol 129:965–971
Bruinenberg PM, De Bot PHM, Van Dijken JP, Scheffers WA (1984) NADH-linked aldose reductase: the key to anaerobic alcoholic fermentation of xylose by yeasts. Appl Microbiol Biotechnol 19:256–260
Delgenes JP, Moletta R, Navarro JM (1986) The effect of aeration on d-xylose fermentation by Pachysolen tannophilus, Pichia stipitis, Kluyveromyces marxianus and Candida shehatae. Biotechnol Lett 8:897–900
Ditzelmüller G, Kubicek CP, Wöhrer W, Röhr M (1984a) Xylose metabolism in Pachysolen tannophilus: purification and properties of xylose reductase. Can J Microbiol 30:1330–1336
Ditzelmüller G, Kubicek CP, Wöhrer W, Röhr M (1984b) Xylitol dehydrogenase from Pachysolen tannophilus. FEMS Microbiol Lett 25:195–198
Ditzelmüller G, Kubicek-Pranz EM, Röhr M, Kubicek CP (1985) NADPH-specific and NADH-specific xylose reduction is catalyzed by two separate enzymes in Pachysolen tannophilus. Appl Microbiol Biotechnol 22:297–299
Du Preez JC, Prior BA (1985) A quantitative screening of some xylose-fermenting yeast isolates. Biotechnol Lett 7:241–246
Du Preez JC, Van der Walt JP (1983) Fermentation of d-xylose to ethanol by a strain of Candida shehatae. Biotechnol Lett 5:357–362
Du Preez JC, Prior BA, Monteiro AMT (1984) The effect of aeration on xylose fermentation by Candida shehatae and Pachysolen tannophilus. A comparative study. Appl Microbiol Biotechnol 19:261–266
Du Preez JC, Bosch M, Prior BA (1986) Xylose fermentation by Candida shehatae and Pichia stipitis: effects of pH, temperature and substrate concentration. Enzyme Microb Technol 8:360–364
Du Preez JC, Van Driessel B, Prior BA (1989) Ethanol tolerance of Pichia stipitis and Candida shehatae strains in fed-batch cultures at controlled low dissolved oxygen levels. Appl Microbiol Biotechnol 30:53–58
Hungate RE (1969) A roll tube method for cultivation of strict anaerobes. In: Norris JR, Ribbons DW (eds) Methods in microbiology, vol 3B. Academic Press, New York London, pp 117–132
Ligthelm ME, Prior BA, Du Preez JC (1988a) The oxygen requirements of yeasts for the fermentation of d-xylose and d-glucose to ethanol. Appl Microbiol Biotechnol 28:63–68
Ligthelm ME, Prior BA, Du Preez JC (1988b) The induction of d-xylose catabolyzing enzymes in Pachysolen tannophilus and the relationship to anaerobic d-xylose fermentation. Biotechnol Lett 10:207–212
Neirinck LG, Maleszka R, Schneider H (1984) The requirement of oxygen for incorporation of carbon from d-xylose and d-glucose by Pachysolen tannophilus. Arch Biochem Biophys 228:13–21
Slininger PJ, Bothast RJ, Van Cauwenberge JE, Kurtzman CP (1982) Conversion of d-xylose to ethanol by the yeast Pachysolen tannophilus. Biotechnol Bioeng 24:371–384
Sliniger PJ, Bothast RJ, Okos MR, Ladisch MR (1985) Comparative evaluation of ethanol production by xylose-fermenting yeasts presented high xylose concentrations. Biotechnol Lett 7:431–436
Slininger PJ, Bolen PL, Kurtzman CP (1987) Pachysolen tannophilus: properties and process considerations for ethanol production from d-xylose. Enzyme Microb Technol 9:5–15
Smiley KL, Bolen PL (1982) Demonstration of d-xylose reductase and d-xylitol dehydrogenase in Pachysolen tannophilus. Biotechnol Lett 4:607–610
Sreenath HK, Chapman TW, Jeffries TW (1986) Ethanol production from d-xylose in batch fermentations with Canadida shehatae: process variables. Appl Microbiol Biotechnol 24:294–299
Van Dijken JP, Scheffers WA (1986) Redox balances in the metabolism of sugars by yeasts. FEMS Microbiol Rev 32:199–224
Verduyn C, Frank J, Van Dijken JP, Scheffers WA (1985a) Multiple forms of xylose reductase in Pachysolen tannophilus CBS4044. FEMS Microbiol Lett 30:313–317
Verduyn C, Van Kleef R, Frank J, Schreuder H, Van Dijken JP, Scheffers WA (1985b) Properties of the NAD(P)H-dependent xylose reductase from the xylose-fermenting yeast Pichia stipitis. Biochem J 226:669–677
Watson NE, Prior BA, Du Preez JC, Lategan PM (1984) Oxygen requirements for d-xylose fermentation to ethanol and polyols by Pachysolen tannophilus. Enzyme Microb Technol 6:447–450
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du Preez, J.C., van Driessel, B. & Prior, B.A. Effect of aerobiosis on fermentation and key enzyme levels during growth of Pichia stipitis, Candida shehatae and Candida tenuis on d-xylose. Arch. Microbiol. 152, 143–147 (1989). https://doi.org/10.1007/BF00456092
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DOI: https://doi.org/10.1007/BF00456092