Summary
The uptake of tyrosine and arginine into wild type and acid phosphatase deficient mutants (pho 1) of Schizosaccharomyces pombe was investigated. All 11 pho 1-alleles tested exhibited a reduced tyrosine uptake and impaired uptake cosegregated with the lack of acid phosphatase activity. Kinetic analyses using wild type cells grown in high phosphate medium (acid phosphatase repressed) and low phosphate medium (acid phosphatase derepressed) showed staturation kinetics for tyrosine with a KM of about 2 × 10−4 M for both media and a V of about 5 nmol min−1mg−1 and 2 nmol min−1mg−1 for derepressed and repressed cells respectively. The pho 1–118 strain completely lacked this saturable uptake system for tyrosine. Preliminary evidence suggests that tyrosine uptake may be via a general amino acid permease system and we conclude that mutations in the structural gene of acid phosphatase which abolish enzyme activity lead to a loss of this uptake system. In contrast to tyrosine, arginine uptake seems not to be significantly affected either by different acid phosphatase levels in wild type cells or by the pho 1–118 mutation.
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Coddington, A., Schweingruber, M.E. Acid phosphatase deficient mutants of Schizosaccharomyces pombe are defective in tyrosine uptake. Curr Genet 11, 113–117 (1986). https://doi.org/10.1007/BF00378202
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DOI: https://doi.org/10.1007/BF00378202