SpringerLink
Log in

Column chromatography of human embryonic hemoglobins

  • Short Communications
  • Published:
Blut Aims and scope Submit manuscript

Summary

Hemoglobin components of human embryonic red blood cells obtained from intact embryos of 20 and 40 mm crown-rump length could be separated by column chromatography on DEAE-Sephacel. The isolation of Hb Gower 1, Hb Gower 2, Hb Portland, Hb F, Hb F1, and Hb A is possible by this method. In the smaller embryos with only 30% of fetal hemoglobin Hb Gower 1 is the predominant component, whereas in the larger ones with 61% of fetal hemoglobin more Hb Gower 2 than Hb Gower 1 is found.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Alter BP (1979) The Gγ:Aγ composition of fetal hemoglobin in fetusses and newborns. Blood 54: 1158–1163

    Google Scholar 

  2. Capp GL, Rigas DA, Jones RT (1967) Hemoglobin Portland 1: A new human hemoglobin unique in structure. Science 157: 65–66

    Google Scholar 

  3. Drescher H, Künzer W (1954) Der Blutfarbstoff des menschlichen Feten. Klin Wochenschr 32: 92

    Google Scholar 

  4. Edman P, Begg G (1967) A protein sequenator. Eur J Biochem 1: 80–91

    Google Scholar 

  5. Hecht F, Motulsky AG, Lemire RJ, Shephard TE (1966) Predominance of hemoglobin Gower 1 in early human embryonic development. Science 152: 91–92

    Google Scholar 

  6. Huehns ER, Dance N, Beaven GH, Keil JV, Hecht F, Motulsky AG (1964) Human embryonic hemoglobins. Nature 201: 1095–1097

    Google Scholar 

  7. Kaltsoya A, Fessas P, Stavropoulos A (1966) Hemoglobins of early human embryonic development. Science 153: 1417–1418

    Google Scholar 

  8. Rovera G, Magarian C, Borun TW (1978) Resolution of hemoglobin subunits by electrophoresis in acid urea polyacrylamide gels containing Triton X-100. Anal Biochem 85: 506–518

    Google Scholar 

  9. Zimmermann CL, Pisano JJ (1977) High-performance liquid chromatography of amino acid derivatives. Methods Enzymol 47: 45–51

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Abteilung Proteinchemie, Max-Planck-Institut für Biochemie, D-8033, Martinsried, Federal Republic of Germany

    T. Kleinschmidt & G. Braunitzer

  2. Department of Human Genetics, Institute for Biological Research “Siniša Stanković”, 29. Novembra 142, YU-11000, Belgrade, Jugoslavia

    D. Sladić-Simić

Authors
  1. T. Kleinschmidt
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Sladić-Simić
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Braunitzer
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kleinschmidt, T., Sladić-Simić, D. & Braunitzer, G. Column chromatography of human embryonic hemoglobins. Blut 45, 283–286 (1982). https://doi.org/10.1007/BF00320197

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00320197

Key words

Search

Navigation