Summary
An optical activity was induced in the region of the absorption bands of acridine orange when the dye became bound to heparin. The magnitudes of induced circular dichroism(ICD) increased with increasing polymer to dye ratio due to the stacking of dyes. ICD of the complex was affected enormously by the temperature because of the rearrangement of aggregated dye molecules bound to heparin to the dimeric molecules. The temperature effect is reversible and the reversibility is very slow.
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Cho, CS., Kang, SW. Temperature effect of the induced circular dichroism in the heparin-acridine orange complex. Polymer Bulletin 15, 369–374 (1986). https://doi.org/10.1007/BF00254858
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DOI: https://doi.org/10.1007/BF00254858