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Quantitative competition of calcium with sodium or magnesium for sorption sites on plasma membrane vesicles of melon (Cucumis melo L.) root cells

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Abstract

The presence of Ca2+ ions in solution is vital for root growth. The plasma membrane is one of the first sites where competition between Ca2+ and other ions occurs. We studied the competition between Ca2+ and Na+ or Mg2+ for sorption sites on the plasma membrane of melon root cells.

Sorption of 45Ca2+ to right-side-out PM vesicles of melon (Cucumis melo L.) roots (prepared by aqueous two-phase partitioning) was studied at various Ca2+ concentrations, in the presence of increasing concentrations of Na+ or Mg2+ chlorides. Experimentally determined amounts of Ca2+ sorbed to the plasma membrane vesicles agreed fairly well with those calculated from a competitive sorption model. The best fit of the model to the experimental data was obtained for an average surface area of 370 Å2 per charge, and binding coefficients for Na+, Mg2+ and Ca2+ of 0.8, 9 and 50 m -1, respectively.

Our results suggest that nonphospholipid components in the plasma membrane contribute significantly to Ca2+ binding. The high affinity of Ca2+ binding to the plasma membrane found in this study might explain the specific role of Ca2+ in relieving salt stress in plant roots.

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Authors and Affiliations

  1. Department of Field and Vegetable Crops, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O.B. 12, 76-100, Rehovot, Israel

    U. Yermiyahu & U. Kafkafi

  2. Seagram Center for Soil and Water Sciences, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O.B. 12, 76-100, Rehovot, Israel

    S. Nir

  3. Institute of Horticulture, The Volcani Center, 50250, Bet Dagan, Israel

    G. Ben-Hayyim

Authors
  1. U. Yermiyahu
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  2. S. Nir
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  3. G. Ben-Hayyim
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  4. U. Kafkafi
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This research was supported by the GIFRID German-Israel fund for research and international development.

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Yermiyahu, U., Nir, S., Ben-Hayyim, G. et al. Quantitative competition of calcium with sodium or magnesium for sorption sites on plasma membrane vesicles of melon (Cucumis melo L.) root cells. J. Membarin Biol. 138, 55–63 (1994). https://doi.org/10.1007/BF00211069

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  • DOI: https://doi.org/10.1007/BF00211069

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