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In vitro induction of tumor-specific immunity. IV. Specific adoptive immunotherapy with cytotoxic T cells induced in vitro to plasmacytoma antigens

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Summary

Specifically activated cytotoxic T cells (CL's) were raised by cocultivation in vitro of normal spleen cells with syngeneic plasmacytoma cells. These CL's were fully active both in vitro in lysing 51Cr-labelled tumor cells and in in vivo Winn assays, in inhibiting the growth of tumor cells in syngeneic mice when inoculated admixed with the tumor cells, even at ratios of 2 CL's to 1 tumor cell. However the same CL populations were only marginally effective in an immunotherapy model in which CL's were injected intravenously and tumor cells subcutaneously or intramuscularly (at a ratio of up to 100 CL/1 tumor cell). It is suggested that the in vitro conditions alter cell surface properties, thereby interfering with normal cell traffic, and that if this problem can be overcome, in vitro educated CL's may constitute a potential source of activated cells for human tumor immunotherapy.

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Authors and Affiliations

  1. Genetics Unit, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia

    Robert C. Burton

  2. Walter and Eliza Hall Institute, P. O. Royal Melbourne Hospital, 3050, Victoria, Victoria, Australia

    Noel L. Warner

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  1. Robert C. Burton
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  2. Noel L. Warner
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National Health and Medical Research Council postgraduate research scholar

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Burton, R.C., Warner, N.L. In vitro induction of tumor-specific immunity. IV. Specific adoptive immunotherapy with cytotoxic T cells induced in vitro to plasmacytoma antigens. Cancer Immunol Immunother 2, 91–99 (1977). https://doi.org/10.1007/BF00200054

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  • DOI: https://doi.org/10.1007/BF00200054

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