Abstract
Congo red was found to be feasible as a microscopic fluorescence indicator of hyphal growth at the single-hypha level. When 1 μm Congo red was applied to mold of Aspergillus niger, the dye was found to a specific cell-wall component, chitin, without causing any inhibitory effect on hyphal growth. The bound Congo red emitted fluorescence at 614 nm. This binding reaction, however, proceeded more slowly than the growing speed of hypha. Consequently the fluorescence intensity was low at the apex where the surface area of the hypha was expanding rapidly. In contrast, as an apex where the growth was retarded, the fluorescence intensity became remarkably high. Therefore growing hyphae could be distinguished from non-growing hyphae by using Congo red.
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References
Katz DF (1991) Human sperm as biomarkers to toxic risk and reproductive health. NIH Res 3:63–67
Oh K, Matsuoka H, Sumita O, Takatori K, Kurata H (1992) Evaluation of antifungal activity of antimycotics by automatic analyzing system. Mycopathologia 118:71–81
Oh K, Matsuoka H, Sumits O, Takatori K, Kurata H (1993a) Automatic evaluation of antifungal volatile compounds on the basis of the dynamic growth process of a single hypha. Appl Microbiol Biotechnol 38:790–794.
Oh K, Matsuoka H, Nemoto Y, Sumita O, Takatori K, Kurata H (1993b) Determination of anti-Aspergillus activity of antifungal agents based on the dynamic growth rate of a single hypha. Appl Microbiol Biotechnol 39:363–367
Pancaldi S, Poli F, Dall'Olio G, Vannini GL (1984) Morphological anomalies induced by Congo red in Aspergillus niger. Arch Microbiol 137:185–187
Park J-C, Nemoto Y, Homma T, **g W, Chen Y, Matsuoka H, Ohno H, Takatori K, Kurata H (1993) Adaptation of Aspergillus niger to short-term salt stress. Appl Microbiol Biotechnol 40:394–398
Taylor DL, Wang Y-L (1989) Fluorescence microscopy of living cells in culture. Part B: quantitative fluorescence microscopy-imaging and spectroscopy. Academic Press, San Diego
Vermeulen CA, Wessels JGH (1984) Ultrastructural differences between wall apices of growing and non-growing hyphae of Schizophyllum commune. Protoplasma 120:123–131
Wood PJ, Fulcher RG (1983) A basis for specific detection and Histochemistry of polysaccharides. J Histochem Cytochem 31:823–826
Yamada S, Cao J, Kurasawa K, Kurata H, Oh K, Matsuoka H (1992) Automatic antifungal analyzing system on the basis of dynamic growth process of a single hypha. Mycopathologia 118:65–69
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Matsuoka, H., Yang, HC., Homma, T. et al. Use of Congo red as a microscopic fluorescence indicator of hyphal growth. Appl Microbiol Biotechnol 43, 102–108 (1995). https://doi.org/10.1007/BF00170630
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DOI: https://doi.org/10.1007/BF00170630