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Correction of experimental metabolic syndrome manifestations in rats by some arginine-containing peptides

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Abstract

Functional characterization of new short glyproline peptides, which are able to provide a regulatory effect on the functional state of the hemostasis system, as well as lipid and carbohydrate metabolism in the body, is an actual task of physiology and medicine. In the present study, we used a model of experimental metabolic syndrome developed in animals due to continuous feeding with high-calorie food. This leads to increased clotting, glucose concentration, low-density lipoprotein cholesterol, triglycerides, and the level of total cholesterol in the blood, which is accompanied by an increase in the body weight of rats. Arginine-containing peptides (Arg-Glu-Arg-Pro-Gly-Pro, Arg-Glu-Arg-Val-Gly-Pro, Arg-Glu-Arg-Gly-Pro) were intranasally administered every 24 h to rats seven times 6 weeks after the development of metabolic syndrome. These peptides provided a unique combined effect on the body, restoring parameters of lipid metabolism, the hemostasis system, and the concentration of blood glucose to normal values. The corrective effect of the studied peptides was detected 20 h after the last administration and was maintained for 168 h even under further feeding of rats with high-calorie diet. The studied glyproline peptides belong to therapeutic normoglycemic and lipid-lowering drugs. They block the accumulation of new fat deposits in the body, and also have anticoagulant and antithrombotic effects in disorders of lipid metabolism. The Arg-Glu-Arg-Pro-Gly-Pro peptide possessed the most pronounced and stable positive effect on the body.

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References

  1. Shubina, T.A., Grigorjeva, M.E., Lyapina, L.A., Obergan, T.Y., Myasoedov, N.F., and Andreeva, L.A, Hypoglycemic and anticoagulant effects of tetrapeptide Pro-Gly-Pro-Leu in hypercholesterolemia, Bull. Exp. Biol. Med., 2014, vol. 158, no. 1, pp. 30–33.

    Article  CAS  PubMed  Google Scholar 

  2. Lyapina, L.A., Grigorieva, M.E, Obergan, T.Yu., Shubina, T.A., Andreeva, L.A., and Myasoedov, N.F., Peptide regulation of metabolic processes under hypercholesterolemia conditions of an organism, Biol. Bull., 2015, vol. 42, no. 6, pp. 546–555.

    Article  CAS  Google Scholar 

  3. Andreeva, L.A., Myasoedov, N.F., Lyapina, L.A., Grigor’eva, M.E., Obergan, T.Y., and Shubina, T.A, Effect of the PRO-GLY-PRO peptide on hemostasis and lipid metabolism in rats with hypercholesterolemia, Dokl. Biol. Sci., 2013, vol. 453, no. 1, pp. 333–335.

    Article  CAS  PubMed  Google Scholar 

  4. Ferrell, J.E. and Martin, G.S, Tyrosine-specific protein phosphorylation is regulated by glycoprotein IIb-IIIa in platelets, Proc. Natl. Acad. Sci. U. S. A., 1989, vol. 86, no. 7, pp. 2234–2238.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Gorbachisky, I., Akpinar, H., and Assimos, D.G, Metabolic syndrome and urological diseases, Rev. Urol., 2010, vol. 12, no. 4, pp. 157–180.

    Google Scholar 

  6. **ao, C., Hsieh, J., Adeli, K., and Lewis, G.F, Gutliver interaction in triglyceride-rich lipoprotein metabolism, Am. J. Physiol. Endocrinol. Metab., 2011, vol. 301, no. 3, pp. E429–E446.

    Article  CAS  PubMed  Google Scholar 

  7. Barkagan, Z.S. and Kostyuchenko, G.I, The metabolic-inflammatory concept of atherothrombosis and new approaches to therapy of patients, Byull. SO RAMN, 2006, no. 2, pp. 132–138.

    Google Scholar 

  8. Watson, T., Arya, A., Sulke, N., and Lip, G.Y.H, Relationship of indices of inflammation and thrombogenesis to arithmia burden in paroxysmal arterial fibrillation, Chest, 2010, vol. 137, no. 4, pp. 869–876.

    Article  PubMed  Google Scholar 

  9. Kong, X., Zhang, D.Y., Wu, H.B., and Li, F.X, Losartan and pioglitazone ameliorate nephropathy in experimental metabolic syndrome rats, Biol. Pharm. Bull., 2011, vol. 34, no. 5, pp. 693–698.

    Article  CAS  PubMed  Google Scholar 

  10. Vila, L., Rebollo, A., Adalsteisson, G.S., Alegre, T.M., Merlos, M., Roglans, N., and Laguna, J.C, Reduction of liver fructokinase expression and improved hepatic inflammation and metabolism in liquid fructose-fed rats at atrovastatin treatment, Toxicol. Appl. Pharmacol., 2011, vol. 251, no. 1, pp. 32–40.

    Article  CAS  PubMed  Google Scholar 

  11. Malinin, V.V., RF Patent 2458935, Byull., no. 23, 2012.

  12. Korkushko, O.V., Chizhova, V.P., Shatilo, V.B., and Khavinson, V.Kh., Efficacy of Pankragen Forte tetrapeptide in elderly people with pre-diabetic disturbances of carbohydrate metabolism, Usp. Gerontol., 2013, vol. 26, no. 2, pp. 297–308.

    Google Scholar 

  13. Zhernakova, Yu.V., Mychka, V.B., and Chazova, I.E, The place of combined therapy in the treatment of patients with metabolic syndrome, Kardiovask. Ter. Prof., 2010, vol. 9, no. 4, pp. 1–4.

    Google Scholar 

  14. Stief, T.W, Inhibition of thrombin in plasma by heparin or arginine, Clin. Appl. Thromb. Hemost., 2007, vol. 13, no. 2, pp. 146–153.

    Article  CAS  PubMed  Google Scholar 

  15. Vasilijevic, A., Buzadžic, B., Korac, A., Petrovic, V., Jankovic, A., and Korac, B., Benefical effects of L-arginine-nitric oxide—producing pathway in rats treated with alloxan, J. Physiol., 2007, vol. 584, no. 3, pp. 921–933.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Myasoedov, N.F., Lyapina, L.A., Grigorjeva, M.E., Obergan, T.Y., Shubina, T.A., and Andreeva, L.A, Mechanism for glyproline protection in hypercholesterolemia, Pathophysiol., 2016, vol. 23, no. 1, pp. 27–33.

    Article  CAS  Google Scholar 

  17. Lyapina, L.A., Grigor’eva, M.E, Obergan, T.Yu., and Shubina, T.A., Teoreticheskie i prakticheskie voprosy izucheniya funktsional’nogo sostoyaniya protivosvertyvayushchei sistemy krovi (Theoretical and Practical Issues of Studying the Functional State of the Anticoagulant System of Blood), Moscow: Advansed Solyushnz, 2012.

    Google Scholar 

  18. Ikee, R., Hamasaki, Y., Oka, M., Maesato, K., Mano, T., Moriya, H., Ohtake, T., and Kobayashi, S, Clinical: High-density lipoprotein cholesterol and left ventricular mass index in peritoneal dialysis, Perit. Dial. Int., 2008, vol. 28, no. 2, pp. 611–616.

    CAS  PubMed  Google Scholar 

  19. Kuznik, B.I, Khavinson, V.Kh., Tarnovskaya, S.I., and Lin’kova, N.S., Epigenetic effect of regulatory peptides on the cytokine profile and hemostatic system, Vestn. Gematol., 2013, vol. 9, no. 2, pp. 29–33.

    Google Scholar 

  20. Glaudemans, A.W., Slart, R.H., Bozzao, A., and Bonanno, E, Molecular imaging in atherosclerosis, Eur. J. Nucl. Med. Mol. Imaging, 2010, vol. 37, no. 12, pp. 2381–2397.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Meade, L.T., Practical use of exenatide and pramlintide for the treatment of type 2 diabetes, J. Pharm. Pract., 2009, vol. 22, no. 6, pp. 540–545.

    Article  Google Scholar 

  22. Shabalina, A.A., Lyapina, L.A., Rochev, D.L., Kostyreva, M.V., Tanashyan, M., and Suslina, T.A, In vitro lipid lowering and fibrinolytic effects of regulatory leucine containing glyprolines in human blood, Biol. Bull., 2015, vol. 42, no. 1, pp. 74–77.

    Article  CAS  Google Scholar 

  23. Hasan, A.A., Wernock, M., Nieman, M., Srikanth, S., Mahdi, F., Krishnan, R., Tulinsky, A., and Schmaier, A., Mechanisms of Arg-Pro-Gly-Phe inhibition of thrombin, J. Physiol. Heart Circ. Physiol., 2003, vol. 285, no. 1, pp. 183–193.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Biology, Moscow State University, Moscow, 119234, Russia

    L. A. Lyapina, M. E. Grigorjeva & T. Y. Obergan

  2. Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russia

    N. F. Myasoedov & L. A. Andreeva

Authors
  1. L. A. Lyapina
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  2. M. E. Grigorjeva
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  3. T. Y. Obergan
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  4. N. F. Myasoedov
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  5. L. A. Andreeva
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Correspondence to L. A. Lyapina.

Additional information

Original Russian Text © L.A. Lyapina, M.E. Grigorjeva, T.Y. Obergan, N.F. Myasoedov, L.A. Andreeva, 2017, published in Vestnik Moskovskogo Universiteta, Seriya 16: Biologiya, 2017, Vol. 72, No. 2, pp. 92–98.

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Lyapina, L.A., Grigorjeva, M.E., Obergan, T.Y. et al. Correction of experimental metabolic syndrome manifestations in rats by some arginine-containing peptides. Moscow Univ. Biol.Sci. Bull. 72, 79–84 (2017). https://doi.org/10.3103/S0096392517020092

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  • DOI: https://doi.org/10.3103/S0096392517020092

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