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The New Data on the Serotonin and FMRFamide Localization in the Nervous System of Opisthorchis felineus Metacercaria

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Abstract

Background

Trematoda Opisthorchis felineus Rivolta, 1884 is the causative agent of dangerous parasite disease—opisthorchiasis, widespread in the Russian Federation. The details of the neuroanatomical localization of the serotoninergic and FMRFamidergic neurotransmitter elements as well as their functional roles remain not studied enough in both adult and larval forms of O. felineus. The studies in this area are important in term of the development of a new pharmacological strategy of the struggle with the causative agent of opisthorchiasis affecting the neuronal signal substances and the function of its nervous system.

Purpose

The aim of this work was the immunocytochemical study of the neurotransmitters serotonin (5-HT, 5-Hydroxitryptamine) and neuropeptide FMRFamide localization in the nervous system of the opisthorchiasis causative agent—O. felineus metacercaria. To study the relationship between the detected neurotransmitters and the muscular elements of the parasite, the muscle staining was carried out simultaneously using fluorophore-conjugated phalloidin.

Methods

The localization of 5-HTergic and FMRFamidergic nerve structures was determined by immunocytochemical method. The staining samples were analyzed using a fluorescent and confocal laser scanning microscopies.

Results

The new data on the presence and distribution of the serotonin-immunopositive (IP)- and FMRFa-IP components in the central and peripheral departments of the nervous system of  O. felineus metacercaria has been obtained. Besides that a number of the new anatomical details of the nervous system organization and of the innervation of the organs and tissues in the investigated parasite have been revealed.

Conclusion

The data obtained on the presence and localization of the 5-HTergic and peptidergic (FMRFamide) components in central and peripheral departments of the nervous system of O. felineus metacercaria elaborated and expanded the existing information about the nervous system as well as the innervations of the tissues and organs in the causative agent of opistchorchiasis.

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Acknowledgements

This work was supported by the Russian Foundation for Basic Research (Grant No.18-04-00349a to Kreshchenko ND, Terenina NB, Mochalova NV).

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Authors and Affiliations

  1. Center of Parasitology, A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russia

    N. B. Terenina, N. V. Mochalova, D. Nefedova, E. L. Voropaeva & S. O. Movsesyan

  2. Institute of Cell Biophysics, Federal Research Center, “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, Pushchino, Moscow Region, Russia

    N. D. Kreshchenko, V. A. Yashin & A. V. Kuchin

  3. W. Stefański Institute of Parasitology, Polish Academy of Sciences, Warsaw, Poland

    A. Demiaszkiewicz

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  1. N. B. Terenina
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  2. N. D. Kreshchenko
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  3. N. V. Mochalova
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Correspondence to N. B. Terenina.

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Terenina, N.B., Kreshchenko, N.D., Mochalova, N.V. et al. The New Data on the Serotonin and FMRFamide Localization in the Nervous System of Opisthorchis felineus Metacercaria. Acta Parasit. 65, 361–374 (2020). https://doi.org/10.2478/s11686-019-00165-2

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