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Facile Preparation of a Hairpin DNA-Gold Nanoparticle Monoconjugate with a Single-Dye Molecule and Lactobionic Acid as Targeting Ligand

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Abstract

We established a new design for a single molecular beacon-conjugated gold nanoparticle, named monoMB-GNP, which showed enhanced fluorescence emission only in the presence of the complementary DNA sequence. MonoMB-GNP also showed no apparent toxicity to NIH/3T3 cells at 1 nM, as determined by the water-soluble tetrazolium assay. Importantly, the lactobionic acid was successfully modified on the surface of monoMB-GNP. The proposed nanoparticle has prospects for use in several applications for targetable molecular beacon strategies.

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Authors and Affiliations

  1. Department of Materials Science and Technology Graduate School of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika, Tokyo, 125-8585, Japan

    Akane Mukaida, Rihito Adachi & Masao Kamimura

  2. Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika, Tokyo, 125-8585, Japan

    Yoshitsugu Akiyama

Authors
  1. Akane Mukaida
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  2. Rihito Adachi
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  3. Yoshitsugu Akiyama
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  4. Masao Kamimura
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Correspondence to Yoshitsugu Akiyama.

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Mukaida, A., Adachi, R., Akiyama, Y. et al. Facile Preparation of a Hairpin DNA-Gold Nanoparticle Monoconjugate with a Single-Dye Molecule and Lactobionic Acid as Targeting Ligand. ANAL. SCI. 37, 785–788 (2021). https://doi.org/10.2116/analsci.20SCN07

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  • DOI: https://doi.org/10.2116/analsci.20SCN07

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