Log in

Anthocyanins profile, total phenolics and antioxidant activity of two Romanian red grape varieties: Fetească neagră and Băbească neagră (Vitis vinifera)

  • Original Paper
  • Published:
Chemical Papers Aims and scope Submit manuscript

Abstract

The phenolic composition of Fetească neagrăand Băbească neagră grapes from Dealul Bujorului vineyard (south-east Romania) was studied using the spectrophotometric and high-performance liquid chromatographic (HPLC) methods. The results revealed significant differences between these cultivars. Total anthocyanins ranged from 0.22–5.98 mg g−1 DW berries in Băbească neagră grapes to 0.54–18.54 mg g−1 DW berries in Fetească neagră. Both cultivars were characterised by an interesting anthocyanin profile for winemaking with a prevalence of malvidin-3-glucoside. The skins and seeds both had small amounts of flavonoids. In contrast with Băbească neagră, Fetească neagră had more flavonoids in the seeds (69 %) than in the skins. Statistically significant correlations were observed between antioxidant activity and total anthocyanin content in both varieties. The antioxidant activity was also found to be highly correlated to the total phenolic compounds content.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Alonso, Á. M., Guillén, D. A., Barroso, C. G., Puertas, B., & García, A. (2002). Determination of antioxidant activity of wine by-products and its correlation with polyphenolic content. Journal of Agricultural and Food Chemistry, 50, 5832–5836. DOI: 10.1021/jf025683b.

    Article  CAS  Google Scholar 

  • Andjelkovic, M., Radovanovic, B., Radovanovic, A., & Andjelkovic, A. M. (2013). Changes in polyphenolic content and antioxidant activity of grapes cv Vranac during ripening. South African Journal of Enology and Viticulture, 34, 147–155.

    CAS  Google Scholar 

  • Arnous A., Makris D. P., & Kefalas P. (2002). Correlation of pigment and flavanol content with antioxidant properties in selected aged regional wines from Greece. Journal of Food Composition and Analysis, 15, 655–665. DOI: 10.1006/jfca.2002.1070.

    Article  CAS  Google Scholar 

  • Balík, J., Kumšta, M., & Rop, O. (2013). Comparison of anthocyanins present in grapes of Vitis vinifera L. varieties and interspecific hybrids grown in the Czech Republic. Chemical Papers, 67, 1285–1292. DOI: 10.2478/s11696-013-0378-9.

    Article  Google Scholar 

  • Burns, J., Mullen, W., Landrault, N., Teissedre, P. L., Lean, M. E. J., & Crozier, A. (2002). Variations in the profile and content of anthocyanins in wines made from Cabernet Sauvignon and hybrid grapes. Journal of Agricultural and Food Chemistry, 50, 4096–4102. DOI: 10.1021/jf011233s.

    Article  CAS  Google Scholar 

  • Capanoglu, E., de Vos, R. C. H., Hall, R. D., Boyacioglu, D., & Beekwilder, J. (2013). Changes in polyphenol content during production of grape juice concentrate. Food Chemistry, 139, 521–526. DOI: 10.1016/j.foodchem.2013.01.023.

    Article  CAS  Google Scholar 

  • de Villiers, A., Vanhoenacker, G., Majek, P., & Sandra, P. (2004). Determination of anthocyanins in wine by direct injection liquid chromatography-diode array detection-mass spectrometry and classification of wines using discriminant analysis. Journal of Chromatography A, 1054, 195–204. DOI: 10.1016/j.chroma.2004.07.087.

    Article  Google Scholar 

  • Dewanto, V., Wu, X. Z., Adom, K. K., & Liu, R. H. (2002). Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. Journal of Agricultural and Food Chemistry, 50, 3010–3014. DOI: 10.1021/jf0115589.

    Article  CAS  Google Scholar 

  • Doshi, P., Adsule, P., & Banargee, K. (2006). Phenolic composition and antioxidant activity in grapevine parts and berries (Vitis vinifera L.) cv. Kishmish Chornyi (Sharad Seedless) during maturation. International Journal of Food Science and Technology, 41, 1–9. DOI: 10.1111/j.1365-2621.2006.01214.x.

    Article  CAS  Google Scholar 

  • Esteban, M. A., Villanueva, M. J., & Lissarrague, J. R. (2001). Effect of irrigation on changes in the anthocyanin composition of the skin of cv Tempranillo (Vitis vinifera L.) grape berries during ripening. Journal of the Science of Food and Agriculture, 81, 409–420. DOI: 10.1002/1097-0010(200103)81:4<409::aid-jsfa830>3.0.co;2-h.

    Article  CAS  Google Scholar 

  • Figueiredo-González, M., Martinez-Carballo, E., Cancho-Grande, B., Santiago, J. L., Martinez, M. C., & Simal-Gándara, J. (2012). Pattern recognition of three Vitis vinifera L. red grapes varieties based on anthocyanin and flavonol profiles, with correlations between their biosynthesis pathways. Food Chemistry, 130, 9–19. DOI: 10.1016/j.foodchem.2011.06.006.

    Article  Google Scholar 

  • Fraige, K., Pereira-Filho, E. R., & Carrilho, E. (2014). Fingerprinting of anthocyanins from grapes produced in Brazil using HPLC-DAD-MS and exploratory analysis by principal component analysis. Food Chemistry, 145, 395–403. DOI: 10.1016/j.foodchem.2013.08.066.

    Article  CAS  Google Scholar 

  • Gómez-Alonso, S., Fernández-González, M., Mena, A., Martínez, J., & García-Romero, E. (2007). Anthocyanin profile of Spanish Vitis vinifera L. red grape varieties in danger of extinction. Australian Journal of Grape and Wine Research, 13, 150–156. DOI: 10.1111/j.1755-0238.2007.tb00245.x.

    Article  Google Scholar 

  • Gutfinger, T. (1981). Polyphenols in olive oils. Journal of the American Oil Chemists Society, 58, 966–968. DOI: 10.1007/bf02659771.

    Article  CAS  Google Scholar 

  • International Organisation of Vine and Wine (2003). Resolution OENO 22/2003. Paris, France: International Organisation of Vine and Wine.

    Google Scholar 

  • Ivanova, V., Stefova, M., Vojnoski, B., Dörnyei, Á., Márk, L., Dimovska, V., Stafilov, T., & Kilár, F. (2011). Identification of polyphenolic compounds in red and white grape varieties grown in R. Macedonia and changes of their content during ripening. Food Research International, 44, 2851–2860. DOI: 10.1016/j.foodres.2011.06.046.

    Article  CAS  Google Scholar 

  • Jia, Z. S., Tang, M. C., & Wu, J. M. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64, 555–559. DOI: 10.1016/s0308-8146(98)00102-2.

    Article  Google Scholar 

  • **, Z. M., He, J. J., Bi, H. Q., Cui, X. Y., & Duan, C. Q. (2009). Phenolic compound profiles in berry skins from nine red wine grape cultivars in Northwest China. Molecules, 14, 4922–4935. DOI: 10.3390/molecules14124922.

    Article  CAS  Google Scholar 

  • Kallithraka, S., Mohdalya, A. A. A., Makris, D. P., & Kefalas, P. (2005). Determination of major anthocyanin pigments in Hellenic native grape varieties (Vitis vinifera sp.): Association with antiradical activity. Journal of Food Composition and Analysis, 18, 375–386. DOI: 10.1016/j.jfca.2004.02.010.

    Article  CAS  Google Scholar 

  • Kammerer, D., Claus, A., Carle, R., & Schieber, A. (2004). Polyphenol screening of pomace from red and white grape varieties (Vitis vinifera L.) by HPLC-DAD-MS/MS. Journal of Agricultural and Food Chemistry, 52, 4360–4367. DOI: 10.1021/jf049613b.

    Article  CAS  Google Scholar 

  • Katalinic, V., Možina, S. S., Skroza, D., Generalić, I., Abramovič, H., Miloš, M., Ljubenkov, I., Piskernik, S., Pezo, I., Terpinc, P., & Boban, M. (2010). Polyphenolic profile, antioxidant properties and antimicrobial activity of grape skin extracts of 14 Vitis vinifera varieties grown in Dalmatia (Croatia). Food Chemistry, 119, 715–723. DOI: 10.1016/j.foodchem.2009.07.019.

    Article  CAS  Google Scholar 

  • Kiselova, Y., Ivanova, D., Chervenkov, T., Gerova, D., Galunska, B., & Yankova, T. (2006). Correlation between the in vitro antioxidant activity and polyphenol content of aqueous extracts from bulgarian herbs. Phytotherapy Research, 20, 961–965. DOI: 10.1002/ptr.1985.

    Article  CAS  Google Scholar 

  • Koyama, K., Ikeda, H., Poudel, P. R., & Goto-Yamamoto, N. (2012). Light quality affects flavonoid biosynthesis in young berries of Cabernet Sauvignon grape. Phytochemistry, 78, 54–64. DOI: 10.1016/j.phytochem.2012.02.026.

    Article  CAS  Google Scholar 

  • Lee, J., Durst, R. W., & Wrolstad, R. E. (2005). Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants and wines by the pH differential method: Collaborative study. Journal of AOAC International, 88, 1269–1278.

    CAS  Google Scholar 

  • Makris, D. P., Psarra, E., Kallithraka, S., & Kefalas, P. (2003). The effect of polyphenolic composition as related to antioxidant capacity in white wines. Food Research International, 36, 805–814. DOI: 10.1016/s0963-9969(03)00075-9.

    Article  CAS  Google Scholar 

  • Mazza, G., Fukumoto, L., Delaquis, P., Girard, B., & Ewert, B. (1999). Anthocyanins, phenolics and colour of Cabernet Franc, Merlot and Pinot Noir wines from British Columbia. Journal of Agricultural and Food Chemistry, 47, 4009–4017. DOI: 10.1021/jf990449f.

    Article  CAS  Google Scholar 

  • Može, Š., Polak, T., Gasóperlin, L., Koron, D., Vanzo, A., Poklar Ulrih, N., & Abram, V. (2011). Phenolics in Slovenian bilberries (Vaccinium myrtillus L.) and blueberries (Vaccinium corymbosum L.). Journal of Agricultural and Food Chemistry, 59, 6998–7004. DOI: 10.1021/jf200765n.

    Article  Google Scholar 

  • Pastrana-Bonilla, E., Akoh, C. C., Sellappan, S., & Krewer, G. (2003). Phenolic content and antioxidant capacity of Muscadine grapes. Journal of Agricultural and Food Chemistry, 51, 5497–5503. DOI: 10.1021/jf030113c.

    Article  CAS  Google Scholar 

  • Poudel, P. R., Tamura, H., Kataoka, I., & Mochioka, R. (2008). Phenolic compounds and antioxidant activities of skins and seeds of five wild grapes and two hybrids native to Japan. Journal of Food Composition and Analysis, 21, 622–625. DOI: 10.1016/j.jfca.2008.07.003.

    Article  CAS  Google Scholar 

  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolourization assay. Free Radical Biology and Medicine, 26, 1231–1237. DOI: 10.1016/s0891-5849(98)00315-3.

    Article  CAS  Google Scholar 

  • Rockenbach, I. I., Gonzaga, L. V., Rizelio, V. M., de Souza Schmidt Gonçalves, A. E., Genovese, M. I., & Fett, R. (2011). Phenolic compounds and antioxidant activity of seed and skin extracts of red grape, Vitis vinifera and Vitis labrusca) pomace from Brazilian winemaking. Food Research International, 44, 897–901. DOI: 10.1016/j.foodres.2011.01.049.

    Article  CAS  Google Scholar 

  • Rodríguez Montealegre, R., Romero Peces, R., Chacon Vozmediano, J. L., Martinez Gascuena, J., & Garcia Romero, E. (2006). Phenolic compounds in skins and seeds often grape Vitis vinifera varieties grown in a warm climate. Journal of Food Composition and Analysis, 19, 687–693. DOI: 10.1016/j.jfca.2005.05.003.

    Article  Google Scholar 

  • Ruberto, G., Renda A., Daquino, C., Amico, V., Spatafora, C., Tringali, C., & De Tommasi, N. (2007). Polyphenol constituents and antioxidant activity of grape pomace extracts from five Sicilian red grape cultivars. Food Chemistry, 100, 203–210. DOI: 10.1016/j.foodchem.2005.09.041.

    Article  CAS  Google Scholar 

  • Stintzing, F. C., & Carle, R. (2004). Functional properties of anthocyanins and betalains in plants, food and in human nutrition. Trends in Food Science & Technology, 15, 19–38. DOI: 10.1016/j.tifs.2003.07.004.

    Article  CAS  Google Scholar 

  • Xu, C. M., Zhang, Y. L., Cao, L., & Lu, J. (2010). Phenolic compounds and antioxidant properties of different grape cultivars grown in China. Food Chemistry, 119, 1557–1565. DOI: 10.1016/j.foodchem.2009.09.042.

    Article  CAS  Google Scholar 

  • Yang, J., Martinson, T. E., & Liu, R. H. (2009). Phytochemical profiles and antioxidant activities of wine grapes. Food Chemistry, 116, 332–339. DOI: 10.1016/j.foodchem.2009.02.021.

    Article  CAS  Google Scholar 

  • Zhu, L., Zhang, Y. L., Deng, J. J., Li, H. R., & Lu, J. (2012). Phenolic concentrations and antioxidant properties of wines made from North American grapes grown in China. Molecules, 17, 3304–3323. DOI: 10.3390/molecules17033304.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Gabriela Râpeanu.

Additional information

Both authors contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Constantin, O.E., Skrt, M., Ulrih, N.P. et al. Anthocyanins profile, total phenolics and antioxidant activity of two Romanian red grape varieties: Fetească neagră and Băbească neagră (Vitis vinifera). Chem. Pap. 69, 1573–1581 (2015). https://doi.org/10.1515/chempap-2015-0163

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1515/chempap-2015-0163

Keywords

Navigation