Abstract—
This paper reports an immunofluorescence confocal microscopy study of the presence and distribution of muscarinic acetylcholine receptor subtypes M1–M5 family in somatic muscle cells and cholinergic synapses of the body wall of the earthworm Lumbricus terrestris. It has been demonstrated that all subtypes of receptors have uneven distribution patterns in the extrasynaptic zones of muscle cells and are also present in the end plate zones. Receptors belonging to subtypes М2–М5, but not to the M1 subtype, can be detected also in the presynaptic membranes of motor nerve terminals.
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REFERENCES
Krause, M. and Wernig, A., The distribution of acetylcholine receptors in the normal and denervated neuromuscular junction of the frog, J. Neurocytol., 1985, vol. 14, p. 765. https://doi.org/10.1007/BF01170827
Kwon, S.E. and Chapman, E.R., Synaptophysin regulates the kinetics of synaptic vesicle endocytosis in central neurons, Neuron, 2011, vol. 70, p. 847. https://doi.org/10.1016/j.neuron.2011.04.001
Minic, J., Molgó, J., Karlsson, E., and Krejci, E., Regulation of acetylcholine release by muscarinic receptors at the mouse neuromuscular junction depends on the activity of acetylcholinesterase, Eur. J. Neurosci., 2002, vol. 15, p. 439. https://doi.org/10.1046/j.0953-816x.2001.01875.x
Nikolsky, E.E., Vyskocil, F., Bukharaeva, E.A., Samigul-lin, D., and Magazanik, L.G., Cholinergic regulation of the evoked quantal release at frog neuromuscular junction, J. Physiol., 2004, vol. 560, p. 77. https://doi.org/10.1113/jphysiol.2004.065805
Nurullin, L.F. and Volkov, E.M., Immunofluorescent identification of α1 isoform subunits of voltage-gated Ca2+-channels of CaV1, CaV2, and CaV3 families in areas of cholinergic synapses of somatic muscles in earthworm Lumbricus terrestris, Cell Tissue Biol., 2020, vol. 14, p. 316. https://doi.org/10.1134/S1990519X20040070
Nurullin, L.F., Mukhitov, A.R., Tsentsevytsky, A.N., Petrova, N.V., Samigullin, D.V., Malomouzh, A.I., Bukharaeva, E.A., Nikolsky, E.E., and Vyskočil, F., Voltage-dependent P/Q-type calcium channels at the frog neuromuscular junction, Physiol. Res., 2011, vol. 60, p. 815. https://doi.org/10.33549/physiolres.932219
Rozkova, E.K., Pharmacology of cholinergic systems in annelids, in International Encyclopedia of Pharmacology and Therapeutics, Sect. 85, Oxford, New York: Elsevier, 1973, vol. 1, p. 169.
Valtorta, F., Pennuto, M., Bonanomi, D., and Benfenati, F., Synaptophysin: leading actor or walk-on role in synaptic vesicle exocytosis?, Bioessays, 2004, vol. 26, p. 445. https://doi.org/10.1002/bies.20012
Volkov, E.M., Nurullin, L.F., Nikolsky, E., and Vyskocil, F., Miniature excitatory synaptic ion currents in the earthworm Lumbricus terrestris body wall muscles, Physiol. Res., 2007, vol. 56, p. 655. https://doi.org/10.33549/physiolres.931269
Volkov, E.M., Nurullin, L.F., Volkov, M.E., Nikolsky, E.E., and Vyskočil, F., Mechanisms of carbacholine and GABA action on resting membrane potential and Na+/K+-ATPase of Lumbricus terrestris body wall muscles, Comp. Biochem. Physiol. A. Mol. Integr. Physiol., 2011, vol. 158, p. 520. https://doi.org/10.1016/j.cbpa.2010.12.016
Walker, R.J., Holden-Dye, L., and Franks, C.J., Physiological and pharmacological studies on annelid and nematode body wall muscle, Comp. Biochem. Physiol. C. Comp. Pharmacol. Toxicol., 1993, vol. 106, p. 49. https://doi.org/10.1016/0742-8413(93)90253-h
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The work was supported by the Research Plan of Kazan State Medical University and in accordance with the state assignment for the Federal Research Center “Kazan Scientific Center of the Russian Academy of Sciences.”
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Translated by E. Martynova
Abbreviations: ACh—acetylcholine; BSA—bovine serum albumin; TMR—tetramethylrhodamine; TMR-α-B—tetramethylrhodamine-α-bungarotoxin; PBS—phosphate-buffered saline.
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Nurullin, L.F., Volkov, E.M. Muscarinic Acetylcholine Receptors Subtypes M1–M5 in the Somatic Muscles of Earthworm Lumbricus terrestris. Cell Tiss. Biol. 16, 589–592 (2022). https://doi.org/10.1134/S1990519X22060049
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DOI: https://doi.org/10.1134/S1990519X22060049