Abstract
The authors have carried out a complex phase micromorphometry of rat erythrocytes with traumatic brain injury (TBI) and the action of cytoflavin (CF) in the post-trauma period. Closed TBI was simulated in animals by means of a free-falling weight on the parieto-occipital region of the skull. One hour after the injury and then for 10 days, the rats were daily intraperitoneally injected with CF at a dose of 0.2 mL/kg. Control animals were injected with isotonic sodium-chloride solution in the same volume. Blood tests were performed 1, 3, 7, and 12 days after the injury. TBI caused an increase in the phase height and phase diameter, volume, area, and average diameter of erythrocytes by 1–7 days relative to the values of the intact group, which was combined with echinocytic and spheroechinocytic transformation of erythrocytes, as well as a shift in the wavelength spectrum of phase portraits towards 650 nm. The effect of CF on the morphometric parameters of erythrocytes in the post-trauma period was manifested in the restoration of the phase height, phase diameter, area, volume, and perimeter of erythrocytes. From three to seven days after the start of CF action, a decrease in the number of echinocytes, cell sphericity, and the wavelength of phase portraits to 600 nm was observed. The possibilities for use of laser-interference microscopy for noninvasive study of erythrocytes in TBI and the action of CF in the post-trauma period are assessed. Analysis of interference images of erythrocytes indicates a decrease in oxidative processes in cells, restoration of the state of hemoglobin, and improved metabolism of erythrocytes under the action of CF in the post-trauma period.
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This work was financially supported by the Russian Foundation for Basic Research, project no. 19-34-90081.
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Statement on the welfare of animals. The experiments were performed in accordance with the requirements of the Ethics Committee of the Institute of Biology and Biomedicine of the Nizhny Novgorod State University. The maintenance of animals and the manipulations carried out with them were carried out in accordance with the regulatory documents presented in the Guide for Care and Use of Laboratory Animals and the requirements of the order of the Ministry of Health of the Russian Federation dated April 1, 2016, no. 199n “On the Approval of Rules of Good Laboratory Practice”.
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Abbreviations: CF—cytoflavin, TBI—traumatic brain injury.
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Deryugina, A.V., Polozova, A.V., Ivashchenko, M.N. et al. Conditions of Erythrocytes during the Post-Traumatic Period of Cranial Cerebral Injury in Rats under the Effect of Cytoflavin: Assay by Laser-Interference Microscopy. Cell Tiss. Biol. 16, 86–91 (2022). https://doi.org/10.1134/S1990519X22010011
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DOI: https://doi.org/10.1134/S1990519X22010011