SpringerLink
Log in

A study of the origin of human glioma based on cell lines and tumor specimens

  • Published:
Cell and Tissue Biology Aims and scope Submit manuscript

Abstract

This study is focused on the search for human glioma “cells of origin.” Specimens of tumor tissue have been assayed with RT-PCR for the expression of molecular markers specific for nerve tissue (NeuN, MOG, MBP, NG2, Olig2, Vimentin, GFAP, Aldh1L1), as well as markers of stem (Oct4, C-Kit) and cancerous stem (CD133) cells. It was found that the expression profiles of these markers were overlapped for different types of gliomas and the type of “cells of origin” cannot be determined. We suggest that more sophisticated culture conditions than traditionally used serum-based media should be applied to study the origin of glioma based on cell lines.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

RT-PCR:

reverse transcription polymerase chain reaction

CSS:

cancer stem cells

References

  • Cabrera, M.C., Hollingsworth, R.E., and Hurt, E.M., Cancer stem cell plasticity and tumor hierarchy, World J. Stem Cells, 2015, vol. 7, pp. 27–36.

    Article  PubMed  PubMed Central  Google Scholar 

  • Calabrese, C., Poppleton, H., Kocak, M., Hogg, T.L., Fuller, C., Hamner, B., Oh, E.Y., Gaber, M.W., Finklestein, D., Allen, M., Frank, A., Bayazitov, I.T., Zakharenko, S.S., Gajjar, A., Davidoff, A., and Gilbertson, R.J., A perivascular niche for brain tumor stem cells, Cancer Cell, 2007, vol. 11, pp. 69–82.

    Article  CAS  PubMed  Google Scholar 

  • Doetsch, F., Caillé, I., Lim, D.A., García-Verdugo, J.M., and Alvarez-Buylla, A., Subventricular zone astrocytes are neural stem cells in the adult mammalian brain, Cell, 1999, vol. 97, pp. 703–716.

    Article  CAS  PubMed  Google Scholar 

  • Fan, F., Bellister, S., Lu, J., Ye, X., Boulbes, D.R., Tozzi, F., Sceusi, E., Kopetz, S., Tian, F., **a, L., Zhou, Y., Bhattacharya, R., and Ellis, L.M., The requirement for freshly isolated human colorectal cancer (CRC) cells in isolating CRC stem cells, Br. J. Cancer, 2015. doi: 10.1038.

    Google Scholar 

  • Fuentealba, L.C., Obernier, K., and Alvarez-Buylla, A., Adult neural stem cells bridge their niche, Cell Stem Cell, 2012, vol. 10, pp. 698–708.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Guo, Y., Liu, S., Wang, P., Zhao, S., Wang, F., Bing, L., Zhang, Y., Ling, E-A., Gao, J., and Hao, A., Expression profile of embryonic stem cell-associated genes Oct4, Sox2 and Nanog in human gliomas, Histopathology, 2011, vol. 59, pp. 763–775.

    Article  PubMed  Google Scholar 

  • Hayat, M.A., Tumors of the Central Nervous System. 1. Gliomas: Glioblastoma (Part 1), Springer, 2011.

    Book  Google Scholar 

  • Ignatova, T.N., Kukekov, V.G., Laywell, E.D., Suslov, O.N., Vrionis, F.D., and Steindler, D.A., Human cortical glial tumors contain neural stem-like cells expressing astroglial and neuronal markers in vitro, Glia, 2002, vol. 39, pp. 193–206.

    Article  PubMed  Google Scholar 

  • Jez, M., Ambady, S., Kashpur, O., Grella, A., Malcuit, C., Vilner, L., Rozman, P., and Dominko, T., Expression and differentiation between OCT4A and its pseudogenes in human ESCs and differentiated adult somatic cells, PLoS One, 2014, vol. 9. doi: 10.1371.

    Google Scholar 

  • Kelly, J.J., Stechishin, O., Chojnacki, A., Lun, X., Sun, B., Senger, D.L., Forsyth, P., Auer, R.N., Dunn, J.F., Cairncross, J.G., Parney, I.F., and Weiss, S., Proliferation of human glioblastoma stem cells occurs independently of exogenous mitogens, Stem Cells, 2009, vol. 27, pp. 1722–1733.

    Article  CAS  PubMed  Google Scholar 

  • Lee, J., Kotliarova, S., Kotliarov, Y., Li, A., Su, Q., Donin, N.M., Pastorino, S., Purow, B.W., Christopher, N., Zhang, W., Park, J.K., and Fine, H.A., Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines, Cancer Cell, 2006, vol. 9, pp. 391–403.

    Article  CAS  PubMed  Google Scholar 

  • Liskovykh, M.A., Chuykin, I.A., Ranjan, A., Safina, D.A., Tolkunova, E.N., Minina, Yu.M., Zhdanova, N.S., Dyban, P.A., Mullins, J.J., Kostyleva, E.I., Chikhirdzhina, E.V., Bader, M., Alenina, N., and Tomilin, A.N., Generation of rat induced pluripotent stem cells: reprogramming and culture medium, Cell Tissue Biol., 2011, vol. 6, 2, pp. 115–121.

    Article  Google Scholar 

  • Louis, D.N., Ohgaki, H., Wiestler, O.D., and Cavenee, W.C., WHO Classification of Tumours of the Central Nervous System, 4th ed., Lyon: IARC, 2007.

    Google Scholar 

  • Magee, J.A., Piskounova, E., and Morrison, S.J., Cancer stem cells: impact, heterogeneity, and uncertainty, Cancer Cell, 2012, vol. 21, pp. 283–296.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Merkle, F.T., Tramontin, A.D., García-Verdugo, J.M., and Alvarez-Buylla, A., Radial glia give rise to adult neural stem cells in the subventricular zone, Proc. Natl. Acad. Sci. USA, 2004, vol. 101, pp. 17528–17532.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Modrek, A.S., Bayin, N.S., and Placantonakis, D.G., Brain stem cells as the cell of origin in glioma, World J. Stem Cells, 2014, vol. 6, pp. 43–52.

    Article  PubMed  PubMed Central  Google Scholar 

  • Rietze, R.L. and Reynolds, B.A., Neural stem cell isolation and characterization, Methods Enzymol., 2006, vol. 419, pp. 3–23.

    Article  CAS  PubMed  Google Scholar 

  • Shackleton, M., Quintana, E., Fearon, E.R., and Morrison, S.J., Heterogeneity in cancer: cancer stem cells versus clonal evolution, Cell, 2009, vol. 138, pp. 822–829.

    Article  CAS  PubMed  Google Scholar 

  • Singh, S.K., Hawkins, C., Clarke, I.D., Squire, J.A., Bayani, J., Hide, T., Henkelman, R.M., Cusimano, M.D., and Dirks, P.B., Identification of human brain tumour initiating cells, Nature, 2004, vol. 432, pp. 396–401.

    Article  CAS  PubMed  Google Scholar 

  • Valent, P., Bonnet, D., De, Maria, R., Lapidot, T., Copland, M., Melo, J.V., Chomienne, C., Ishikawa, F., Schuringa, J.J., Stassi, G., Huntly, B., Herrmann, H., Soulier, J., Roesch, A., Schuurhuis, G.J., Wöhrer, S., Arock, M., Zuber, J., Cerny-Reiterer, S., Johnsen, H.E., Andreeff, M., and Eaves, C., Cancer stem cell definitions and terminology: the devil is in the details, Nat. Rev. Cancer, 2012, vol. 12, pp. 767–775.

    Article  CAS  PubMed  Google Scholar 

  • Velpula, K.K., Dasari, V.R., Tsung, A.J., Dinh, D.H., and Rao, J.S., Cord blood stem cells revert glioma stem cell EMT by down regulating transcriptional activation of Sox2 and Twist1, Oncotarget, 2011, vol. 2, pp. 1028–1042.

    Article  PubMed  PubMed Central  Google Scholar 

  • Visvader, J.E., Cells of origin in cancer, Nature, 2011, vol. 469, pp. 314–322.

    Article  CAS  PubMed  Google Scholar 

  • Wang, J., Sakariassen, P.Ø., Tsinkalovsky, O., Immervoll, H., Bøe, S.O., Svendsen, A., Prestegarden, L., Røsland, G., Thorsen, F., Stuhr, L., Molven, A., Bjerkvig, R., and Enger, P.Ø., CD133 negative glioma cells form tumors in nude rats and give rise to CD133 positive cells, Int. J. Cancer, 2008, vol. 122, pp. 761–768.

    Article  CAS  PubMed  Google Scholar 

  • Wu, B., Sun, C., Feng, F., Ge, M., and **a, L., Do relevant markers of cancer stem cells CD133 and nestin indicate a poor prognosis in glioma patients? A systematic review and meta-analysis, J. Exp. Clin. Cancer Res., 2015, vol. 34, no. 44. doi: 10.1186.

    Article  PubMed  PubMed Central  Google Scholar 

  • Yuan, X., Curtin, J., **ong, Y., Liu, G., WaschsmannHogiu, S., Farkas, D.L., Black, K.L., and Yu, J.S., Isolation of cancer stem cells from adult glioblastoma multiforme, Oncogene, 2004, vol. 23, pp. 9392–9400.

    Article  CAS  PubMed  Google Scholar 

  • Zong, H., Verhaak, R.G., and Canoll, P., The cellular origin for malignant glioma and prospects for clinical advancements, Expert Rev. Mol. Diagn., 2012, vol. 12, pp. 383–394.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, St. Petersburg, 194064, Russia

    G. V. Timin & E. N. Tolkunova

  2. Polenov Russian Scientific Research Institute of Neurosurgery (Federal Almazov Northwest Medical Research Center), St. Petersburg, 191014, Russia

    Yu. S. Lakhina & D. A. Gulyaev

Authors
  1. G. V. Timin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yu. S. Lakhina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. A. Gulyaev
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. N. Tolkunova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. N. Tolkunova.

Additional information

Original Russian Text © G.V. Timin, Yu.S. Lakhina, D.A. Gulyaev, E.N. Tolkunova, 2015, published in Tsitologiya, 2015, Vol. 57, No. 12, pp. 893–898.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Timin, G.V., Lakhina, Y.S., Gulyaev, D.A. et al. A study of the origin of human glioma based on cell lines and tumor specimens. Cell Tiss. Biol. 10, 100–105 (2016). https://doi.org/10.1134/S1990519X16020103

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1990519X16020103

Keywords

Search

Navigation