Abstract
The development of microbial community in a soddy-podzolic soil (Albic Retisol) on the roots of common barley (Hordeum vulgare L.) was studied. Morphometric characteristics of plant roots (length and surface area) were obtained with the help of scanning technique, and characteristics of root hairs were obtained by microscopy. The number of bacteria in barley rhizosphere and rhizoplane was estimated using the method of cascade filtration. A significant contribution (about 30%) of root hairs to the total surface area of barley root system was demonstrated. The germination of barley seeds was accompanied by an increase in the population of bacteria in the rhizosphere and rhizoplane by almost an order of magnitude; during the stage of the development of lateral roots, the density of bacterial cells on the root surface decreased by almost three times in comparison with the initial stage of root development.
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REFERENCES
D. G. Zvyagintsev, “Ultrasonic pretreatment of soils for quantitative counting of microorganisms,” Vestn. Mosk. Univ., Ser. Biol., Pochv., No. 3, 127–129 (1968).
P. A. Kozhevin, L. M. Polyanskaya, and D. G. Zvyagintsev, “Development of different microorganisms in soil,” Mikrobiologiya 48, 490–494 (1979).
L. V. Kravchenko, A. I. Shaposhnikov, and N. M. Makarova, “The composition of root exudates of the plants and its change in the rhizosphere affected by soil microflora,” S-kh. Biol., No. 3, 71–75 (2011).
N. A. Krasil’nikov, Soil Microorganisms and Higher Plants (Academy of Sciences of the Soviet Union, 1958) [in Russian].
World Reference Base for Soil Resources 2014, International Soil Classification System for Naming Soils and Creating Legends for Soil Maps (Food and Agriculture Organization of the United Nations, Rome, 2015; Moscow, 2017).
N. E. Pavlovskaya, E. V. Kostomicheva, E. S. Kuleshova, I. V. Gor’kova, and I. N. Gagarina, “Barley as a source of antibiotics,” Vestn. Orlovsk. Gos. Agrar. Univ., No. 4 (37), 70–73 (2012).
L. M. Polyanskaya, R. B. Gorodnichev, and D. G. Zvyagintsev, “Sizes of bacterial cells in soils determined by cascade filtration technique,” Biol. Bull. 40, 130–137 (2013).
L. M. Polyanskaya, T. G. Mirchink, P. A. Kozhevin, and D. G. Zvyagintsev, “Change of the complex of soil micromycetes during microbial successions,” Mikrobiologiya 59, 349–354 (1990).
L. M. Polyanskaya, M. Kh. Orazova, T. G. Mirchink, and D. G. Zvyagintsev, “Dynamics of population and the structure of microbial complex in rhizosphere zone of pea plants,” Mikrobiologiya 63, 314–325 (1994).
L. M. Polyanskaya, I. P. Pinchuk, and D. G. Zvyagintsev, “Assessment of the number, biomass, and cell size of bacteria in different soils using the “cascade” filtration method,” Eurasian Soil Sci. 48, 288–293 (2015). doi 10.1134/S1064229315030096
L. M. Polyanskaya, A. L. Stepanov, and K. V. Chakmazyan, “The impact of hydrogen emission on the structure of soil microbial biomass,” Eurasian Soil Sci. 50, 57–63 (2017). doi 10.1134/S1064229317010112
S. Skugoreva, The effect of mercury nitrate (III) on the growth and development of barley sprouts grown on hydroponics,” in Proceedings of All-Russian Scientific School “Relevant Problems of Regional Ecological Monitoring: Scientific and Educational Aspects” (Kirov, 2005), pp. 81–84.
A. I. Shaposhnikov, A. A. Belimov, L. V. Kravchenko, and D. M. Vivanko, “Interaction of rhizosphere bacteria with the plants: formation and efficiency of associative symbioses,” S-kh. Biol., No. 3, 16–22 (2011).
E. V. Shein and V. M. Goncharov, Agrophysics (Feniks, Rostov-on-Don, 2006) [in Russian].
H. Bais, T. Weir, L. G. Perry, S. Gilroy, and J. M. Vivanco, “The role of root exudates in rhizosphere interactions with plants and other organisms,” Annu. Rev. Plant. Biol. 57, 233–266 (2006).
D. Barthélémy and Y. Caraglio, “Plant architecture: a dynamic, multilevel and comprehensive approach to plant form, structure and ontogeny,” Ann. Bot. 99 (3), 375–407 (2007).
E. Blagodatskaya and Y. Kuzyakov, “Active microorganisms as drivers of dynamic processes in soil: integration of basic teaching into research,” Soil Biol. Biochem. 67, 192–211 (2013).
P. Dennis, A. J. Miller, and P. R. Hirsch, “Are root exudates more important than other sources of rhizodeposits in structuring rhizosphere bacterial communities?” FEMS Microbiol. Ecol. 72, 313–327 (2010).
R. A. Dixon, “Natural products and plant disease resistance,” Nature 411, 843–847 (2001).
S. Dorlodot, B. Forster, et al., “Root system architecture: opportunities and constraints for genetic improvement of crops,” Trends Plant Sci. 10, 474–481 (2007).
T. W. M. Fan, A. N. Lane, J. Pedler, D. Crowley, and R. M. Higashi, “Comprehensive analysis of organic ligands in whole root exudates using nuclear magnetic resonance and gas chromatography—mass spectrometry,” Anal. Biochem. 251, 57–68 (1997).
T. S. Gahoonia, D. Care, and N. E. Nielsen, “Root hairs and phosphorus acquisition of wheat and barley cultivars,” Plant Soil 191, 181–188 (1997).
T. S. Gahoonia and N. E. Nielsen, “Barley genotypes with long root hairs sustains high grain yields in low-P field,” Plant Soil 262, 55–62 (2004).
C. Hackett and D. A. Rose, “Model of the extension and branching of a seminal root of barley and its use for studying relations between root dimensions,” Aust. J. Biol. Sci. 25, 669–679 (1972).
A. Jungk, “Root hairs and the acquisition of plant nutrients from soil,” Plant Natr. Soil Sci. 164, 121–129 (2001).
N. P. Kirillova, G. A. Stasevich, P. A. Kozhevin, and D. G. Zvyagintsev, “Bacterial population-dynamics in a soil-plant system,” Microbiology (Moscow) 50, 94–98 (1981).
S. Loeppmann, M. Semenov, E. Blagodatskaya, and Y. Kuzyakov, “Substrate quality affects microbial- and enzyme activities in rooted soil,” J. Plant Nutr. Soil. Sci. 179, 39–47 (2016).
Z. Ma, N. V. Bykova, and A. U. Igamberdiev, “Cell signaling mechanisms and metabolic regulation of germination and dormancy in barley seeds,” Crop J. 5, 459–477 (2017).
S. Smith and I. D. Smet, “Root system architecture: insights from Arabidopsis and cereal crops,” Philos. Trans. R. Soc., B 367, 1441–1452 (2012).
M. I. Timonin, “The interaction of higher plants and soil microorganisms. I. Microbial populations of rhizosphere of seedlings of certain cultivated plants,” Can. J. Res. 18, 307–317 (1940).
G. Vercambre, L. Pagès, C. Doussan, and R. Habib, “Architectural analysis and synthesis of the plum tree root system in an orchard using a quantitative modeling approach,” Plant Soil. 251, 1–11 (2003).
T. S. Walker, H. P. Bais, E. Grotewold, and J. M. Vivanco, “Root exudation and rhizosphere biology,” Plant Physiol. 132, 44–51 (2003).
M. H. Weisenseel, A. Dorn, and L. Jaffe, “Natural H+ currents traverse growing roots and root hairs of barley (Hordeum vulgare L.),” Plant Physiol. 64, 512–518 (1979).
R. D. Willows, P. Worden, and M. Mirzaei, “Barley grain proteomics,” in Proteomics in Food Science: From Farm to Fork (Elsevier, London, 2017), pp. 75–88.
WinRHIZO: image analysis system. http://www.regentinstruments.com/assets/winrhizo_about.html.
J. Zhu, P. A. Ingram, P. N. Benfey, and T. Elich, “From lab to field, new approaches to phenoty** root system architecture,” Curr. Opin. Plant Biol. 14, 310–317 (2011).
ACKNOWLEDGMENTS
This work was supported by Russian Science Foundation, project no. 14-50-00029.
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Translated by T. Chicheva
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Pinchuk, I.P., Polyanskaya, L.M., Kirillova, N.P. et al. Specific Features of the Microbial Community Development in Soddy-Podzolic Soil in the Course of Barley (Hordeum vulgare L.) Growing. Eurasian Soil Sc. 51, 1480–1486 (2018). https://doi.org/10.1134/S1064229318120098
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DOI: https://doi.org/10.1134/S1064229318120098