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Effects of Insulin-Like Growth Factor 1 on the In Vitro Development of Mouse Embryos after Cryopreservation

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Abstract

Cryopreservation of gametes and embryos is widely used for mammalian genome resource banking. Effects of insulin-like growth factor-1 (IGF-1) on the in vitro development of cleavage stage CD1 mouse embryos after their cryopreservation were studied. The mouse embryos developed in vivo up to the 4–6-cell stage were frozen in a programmable freezer according to standard protocol with 10% propylene glycol as a cryoprotectant and thereafter were thawed and cultured in vitro in KSOM for 48 h. The percentage of embryos developed up to blastocyst stage in culture medium supplemented with IGF-1 (40 ng/mL) was higher as compared to controls (78.9 and 47.4%, respectively, p < 0.01). Moreover, blastocysts developed in the presence of IGF-1 contained more cells as compared to controls (79.8 ± 3.9 and 58.6 ± 3.6 cells, respectively, p < 0.001). However, the index of nuclear fragmentation did not differ between these groups. Experimental results presented here revealed the stimulating effects of IGF-1 on the mouse preimplantation embryos after their cryopreservation.

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ACKNOWLEDGMENTS

We are grateful to the Center for Collective Use Genetic Resources of Laboratory Animals (http://spf.bionet.nsc.ru) and the Center for Collective Use Microscopic Analysis of Biological Objects (Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences) (http://www.bionet.nsc.ru/microscopy).

Funding

This work was supported by the Russian Foundation for Basic Research (no. 19-016-00025, budget project no. 0324-2019-0041-C-01) using the equipment of the Center for Genetic Resources of Laboratory Animals, Federal Research Center (Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences) and supported by the Ministry of Education and Science of Russia (Unique project identifier RFMEFI62119X0023).

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Russian Academy of Sciences, Siberian Branch, 630090, Novosibirsk, Russia

    E. Yu. Brusentsev, E. A. Kizilova, T. N. Igonina, S. V. Ranneva & S. Ya. Amstislavsky

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  1. E. Yu. Brusentsev
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  2. E. A. Kizilova
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  3. T. N. Igonina
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  4. S. V. Ranneva
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  5. S. Ya. Amstislavsky
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Correspondence to S. Ya. Amstislavsky.

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Conflict of interest. The authors declare that they have no conflict of interests.

Statement on the welfare of animals. When performing this study, all manipulations carried out with experimental animals, methods of anesthesia, euthanasia and animal care before and after experimental interventions were in accordance with international bioethics standards.

AUTHOR CONTRIBUTION INFORMATION

E.Yu. Brusentsev, T.N. Igonina, and S.Ya. Amstislavsky came up with and developed an experiment design. E.Yu. Brusentsev, T.N. Igonina, and S.V. Ranneva received preimplantation embryos, carried out their freezing-thawing, and their cultivation in vitro. E.A. Kizilova performed DAPI staining, counting the number of interphase nuclei, their fragments and metaphase plates in develo** blastocysts. E.Yu. Brusentsev, T.N. Igonina, S.V. Ranneva, and S.Ya. Amstislavsky participated in data processing. E.Yu. Brusentsev, T.N. Igonina, and S.Ya. Amstislavsky participated in writing the text of the article. All authors participated in the discussion of the results.

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Brusentsev, E.Y., Kizilova, E.A., Igonina, T.N. et al. Effects of Insulin-Like Growth Factor 1 on the In Vitro Development of Mouse Embryos after Cryopreservation. Russ J Dev Biol 52, 120–124 (2021). https://doi.org/10.1134/S1062360421020028

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  • DOI: https://doi.org/10.1134/S1062360421020028

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