Abstract
Characteristics of tryptic peptides that provide the detection and identification of these compounds and corresponding proteins by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry with α-cyano-4-hydroxycinnamic acid as a matrix are determined and discussed. Twenty-five proteins are identified; the features of reliably identified peptides are compared to those of the compounds that might be produced by trypsinolysis but were not detected. Two key factors enhancing a possibility of the detection of tryptic peptides are determined. One refers to the basicity of peptides in the gas phase, which is the highest for peptides with the C-terminal arginine residue; this reason for the emergence of significant analytical signals is well known. Another factor contributing to the ionization of peptides in study under MALDI conditions is their hydrophilicity. This result did not correlate with the most conclusions of earlier relevant studies discussed in the article.
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ACKNOWLEDGMENTS
The results of protein identification obtained in the Laboratory of Biomedical and Pharmaceutical Mass Spectrometry of the Institute of Experimental Medicine were used. The authors are grateful to the colleagues in the Institute of Experimental Medicine, who provided relevant samples for analysis: Dr. Sci. T.V. Gupalova, Dr. Sci. A.V. Dmitriev, Ph. D. E.V. Kuleshevich, Dr. Sci. A.I. Orlov, M.D. A.N. Suvorov, and M. D. M.M. Shavlovskii. The authors are also grateful to Prof. M.M. Shavlovskii for valuable advice and Dr. Sci. O.V. Shamova for discussing the manuscript of the article and the critical comments made.
Funding
The work was performed within the framework of the State Assignment for Performing Fundamental Research in the Program for Basic Research in State Academies of Sciences for 2013–2020, Section 76 “Development of Molecular Profiling Methods for Predicting the Risks of Develo** Socially Significant Diseases.”
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Milman, B.L., Solov’eva, A.V., Lugovkina, N.V. et al. Features of Tryptic Peptides Providing Their Detection and Identification by MALDI Mass Spectrometry. J Anal Chem 74, 1286–1295 (2019). https://doi.org/10.1134/S1061934819130094
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DOI: https://doi.org/10.1134/S1061934819130094