SpringerLink
Log in

Validated reversed phase HPLC method for determination of pioglitazone hydrochloride in bulk drug and tablet formulations

  • Articles
  • Published:
Journal of Analytical Chemistry Aims and scope Submit manuscript

Abstract

An accurate, sensitive and precise HPLC method was developed and validated for the routine analysis of pioglitazone hydrochloride in dosage forms. The analyte was chromatographed on a C-18 column using a mixture of acetonitrile and ammonium acetate buffer (pH 5.0) as mobile phase in ratio 60: 40 (v/v) at flow rate 1.0 mL/min. Linearity range was found to be 10–100 μg/mL with a correlation coefficient r 2 = 0.9984. The method was validated for precision, accuracy, robustness, specificity and sensitivity, using bulk drug samples. Application of method in assay of bulk drug and tablets revealed mean recoveries range from 99.88–100.32%. Due to its simplicity, rapidity, high precision and accuracy, the proposed method may be used for determining pioglitazone hydrochloride in bulk and dosage forms.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Prigeon, R.L., Kahn, S.E., and Porte, J.D., J. Clin. Endocrinol. Metab., 1998, vol. 83, p. 819.

    CAS  Google Scholar 

  2. Cavaghan, M.K., Ehrmann, D.A., Byrne, M.M., and Polonsky, K.S., J. Clin. Invest., 1997, vol. 100, p. 530.

    Article  CAS  Google Scholar 

  3. Sevgi, T.U. and Fikriye, T.E., Anal. Lett., 2009, vol. 42, p. 2254.

    Article  Google Scholar 

  4. Shankar, M.B., Modi, V.D., Shah, D.A., Bhatt, K.K., Mehta, R.S., and Geetha, M., J. AOAC Int., 2005, vol. 88, no. 4, p. 1167.

    CAS  Google Scholar 

  5. Radhakrishna, T., Sreenivas, R.D., and Om, R.G., J. Pharm. Biomed. Anal., 2002, vol. 29, p. 593.

    Article  CAS  Google Scholar 

  6. Zhong, W.Z. and Williams, M.G., J. Pharm. Biomed. Anal., 1996, vol. 14, no. 4, p. 465.

    Article  CAS  Google Scholar 

  7. Ho, E.N., Yiu, K.C., Wan, T.S., Stewart, B.D., and Watkins, K.L., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2004, vol. 811, no. 1, p. 65.

    Article  CAS  Google Scholar 

  8. Lin, Z.J., Ji, W., Desai, K.D., and Shum, L., J. Pharm. Biomed. Anal., 2003, vol. 33, no. 1, p. 101.

    Article  CAS  Google Scholar 

  9. Wang, M. and Miksa, I.R., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2007, vol. 856, no. 1, p. 318.

    Article  CAS  Google Scholar 

  10. Yamashita, K., Murakami, H., Okuda, T., and Motohashi, M., J. Chromatogr. B: Biomed. Sci. Appl., 1996, vol. 677, no. 1, p. 141.

    Article  Google Scholar 

  11. Souri, E., Jalalizadeh, H., and Saremi, S., J. Chromatogr. Sci., 2008, vol. 46, no. 9, p. 809.

    Article  CAS  Google Scholar 

  12. Zhong, W.Z. and Lakings, D.B., J. Chromatogr., 1989, vol. 490, no. 2, p. 377.

    Article  CAS  Google Scholar 

  13. Sripalakit, P. and Neamhom, P., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2006, vol. 843, no. 2, p. 164.

    Article  CAS  Google Scholar 

  14. Kolte, B.L., Raut, B.B., Deo, A.A., Bagool, M.A., and Shinde, D.B., J. Chromatogr. Sci., 2004, vol. 42, p. 27.

    Article  CAS  Google Scholar 

  15. Jain, D., Jain, S., Jain, D., and Amin, M., J. Chromatogr. Sci., 2008, vol. 46, p. 501.

    Article  CAS  Google Scholar 

  16. Mostafa, G.A. and Majed, A., J. Pharm. Biomed. Anal., 2008, vol. 48, no. 1, p. 57.

    Article  CAS  Google Scholar 

  17. ICH. Validation of Analytical Procedure; Methodology, International Conference on Harmonization of Technical Requirements for Registration of Pharmaceutical for Human Use, Geneva, 2000.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, M. B. Road, New Delhi, 110062, India

    Prem Sunder Prasad, Syed Sarim Imam, Mohammed Aqil, Mohammad Rizwan, Yasmin Sultana & Asgar Ali

Authors
  1. Prem Sunder Prasad
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Syed Sarim Imam
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mohammed Aqil
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mohammad Rizwan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yasmin Sultana
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Asgar Ali
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mohammed Aqil.

Additional information

The article is published in the original.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Prasad, P.S., Imam, S.S., Aqil, M. et al. Validated reversed phase HPLC method for determination of pioglitazone hydrochloride in bulk drug and tablet formulations. J Anal Chem 70, 744–746 (2015). https://doi.org/10.1134/S106193481506012X

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S106193481506012X

Keywords

Search

Navigation