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The effect of Low Temperature and Nitrogen Starvation on the Morphological and Physiological Characteristics of Two Strains of Green Microalgae of the Genus Lobosphaera sp. (Chlorophyta, Trebouxiophyceae)

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Abstract

The effect of nitrogen starvation and, for the first time, low temperature, as well as their simultaneous effect, on the physiology and ultrastructure of cells of microalgae of the genus Lobosphaera (Chlorophyta, Trebouxiophyceae) was studied. Nitrogen deficiency in both strains led to a decrease in the content of chlorophyll by three times and an increase in the proportion of carotenoids by two times. A decrease in the content of both chlorophyll and carotenoids was observed at +10°C. The simultaneous effect of two factors resulted in a threefold decrease in the chlorophyll content in NAMSU 924/2 and a sixfold decrease in NAMSU (CALU) 1497; the proportion of carotenoids in both strains decreased by 1.5–2 times. Data on ultrastructural changes in cells of microalgae of the genus Lobosphaera under the influence of stress factors have been obtained. A similar nature of the response in both strains to stress conditions was noted. Nitrogen deficiency led to the accumulation of numerous lipid droplets in the cytoplasm of cells along the cell wall. Long-term incubation on a nitrogen-free medium led to the filling of the entire volume of cells with lipid droplets, disassembly of the membrane system of chloroplasts, that reduction in sizeand being located between densely lying lipid droplets. At low temperatures, the number of thylakoids decreased, while the interthylakoid space and the size of chloroplasts increased. With simultaneous exposure to nitrogen starvation and low temperature, numerous lipid droplets accumulated, the number of thylakoids decreased, the interthylakoid space and the size of the chloroplast increased, which was noted under separate exposure to stress factors. The pyrenoid in both strains did not undergo significant changes in all cases.

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Funding

This work was financially supported by the Russian Science Foundation, grant no. 20–74–10028 and performed within the framework of the Interdisciplinary Scientific and Educational School of Moscow University Molecular Technologies of Living Systems and Synthetic Biology.

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  1. Moscow State University, Moscow, Russia

    K. A. Shibzukhova, O. B. Chivkunova & E. S. Lobakova

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  1. K. A. Shibzukhova
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Correspondence to K. A. Shibzukhova.

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Translated by M. Shulskaya

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Shibzukhova, K.A., Chivkunova, O.B. & Lobakova, E.S. The effect of Low Temperature and Nitrogen Starvation on the Morphological and Physiological Characteristics of Two Strains of Green Microalgae of the Genus Lobosphaera sp. (Chlorophyta, Trebouxiophyceae). Russ J Plant Physiol 70, 55 (2023). https://doi.org/10.1134/S1021443722700091

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