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UV-B radiation induces biphasic burst of hydrogen peroxide in mesophyll Chlorella vulgaris

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Abstract

The fluctuations of hydrogen peroxide and total hydroperoxides were followed up at different time points in UV-B-irradiated cell suspension cultures of the green microalga Chlorella vulgaris. A biphasic burst of hydrogen peroxide was recorded, while cell viability was strongly reduced. This pattern is similar to the two wave oxidative burst in plants expressing hypersensitive response to pathogens and PCD and implies the occurrence of a PCD-like response in UV-B-exposed unicellular green algae.

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Abbreviations

EPR:

electron paramagnetic resonance

PAR:

4-(2-pyridylazo)resorcinol

PCD:

programmed cell death

TCA:

trichloroacetic acid

TTC:

triphenyl tetrazolium chloride

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Authors and Affiliations

  1. Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, ul. Acad. G. Bonchev 21, 1113, Sofia, Bulgaria

    A. M. Edreva, I. D. Pouneva & E. Zh. Gesheva

Authors
  1. A. M. Edreva
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  2. I. D. Pouneva
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  3. E. Zh. Gesheva
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Correspondence to A. M. Edreva.

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Edreva, A.M., Pouneva, I.D. & Gesheva, E.Z. UV-B radiation induces biphasic burst of hydrogen peroxide in mesophyll Chlorella vulgaris . Russ J Plant Physiol 62, 219–223 (2015). https://doi.org/10.1134/S1021443715010069

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