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The Influence of Adiponectin on Production of Apolipoproteins A-1 and E by Human Macrophages

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Abstract

Adiponectin is an adipose tissue hormone affecting energy and lipoprotein metabolism and modulating inflammatory responses. However, the role of this adipokine in atherogenesis remains poorly understood. The aim of this study was to investigate the effect of adiponectin on the production of apolipoproteins (apo) A-1 and E by human macrophages (MP). The study was conducted on macrophage-like cells of the THP-1 cell line of two differentiation terms, 3 and 5 days (3d and 5d). Adiponectin (10 μg/mL) stimulated the expression of apoA-1 gene at the mRNA level in 5d MP, but not in 3d MP. The level of apoE mRNA in MP under the action of adiponectin was not affected. Adiponectin suppressed macrophage TNF gene expression, while it induced the expression of IL-10 gene in 5d MP. The secreted levels of apoA-1 and apoE proteins under the action of adiponectin in macrophages of both periods of differentiation remained unchanged, while the level of the surface apoA-1 protein in 5d MP was decreasing. Incubation of 5d MP with the PPARα nuclear receptor antagonist MK-886 or with the nuclear receptor LXR agonist TO-901317 resulted in cancellation of the stimulating effect of adiponectin on apoA-1 gene expression. These data indicate that adiponectin, in addition to its anti-inflammatory action, has a modulating effect on production of apoA-1 by macrophages. The latter is probably one of the mechanisms of the influence of this adipokine on atherogenesis.

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ACKNOWLEDGMENTS

The authors are grateful to I.V. Kudryavtsev and M.K. Serebryakova (Department of Immunology of the IEM) for help with flow cytometry and A.D. Denisenko (Department of Biochemistry, IEM) for valuable critical comments.

Funding

The study was financially supported by the Russian Foundation for Basic Research (project no. 15-04-07918).

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Authors and Affiliations

  1. Institute of Experimental Medicine, 197376, St. Petersburg, Russia

    D. A. Tanyanskiy, A. S. Trulioff, E. V. Ageeva, A. A. Nikitin, V. S. Shavva & S. V. Orlov

  2. St. Petersburg State University, 199034, St. Petersburg, Russia

    S. V. Orlov

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  1. D. A. Tanyanskiy
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  2. A. S. Trulioff
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Correspondence to D. A. Tanyanskiy.

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In this work, we did not use humans or animals as objects of research. The authors declare they have no conflict of interest.

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Abbreviations: apo, apolipoprotein; BSA, bovine serum albumin; ELISA, enzyme-linked immunosorbent assay; LDL, low density lipoproteins; MP, macrophages; RT-PCR, Real-time reverse transcription PCR; AdipoR, adiponectin receptors; AMPK, AMP-activated protein kinase; DMSO, dimethyl sulfoxide; FBS, fetal bovine serum; IL-10, interleukin-10; LXR, liver X-receptors; PBS, phosphate buffered saline; PMA, phorbol-12-myristate-13-acetate; PPAR, peroxisome proliferator-activated receptor; TNF, tumor necrosis factor.

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Tanyanskiy, D.A., Trulioff, A.S., Ageeva, E.V. et al. The Influence of Adiponectin on Production of Apolipoproteins A-1 and E by Human Macrophages. Mol Biol 55, 637–643 (2021). https://doi.org/10.1134/S0026893321030122

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