Abstract
The transcription factor KAISO is important for proper development of animal embryos. In the cell, KAISO regulates cell division and apoptosis. KAISO is abundant in the central nervous system. Here we describe the effects of Zbtb33 gene knockout on the transcription of several genes that regulate the development of the central nervous system, including Fgf9, Fgfr3, Sox9, Sox2, c-Myc, NeuroD1 and FoxG1. These genes are related to the Wnt/β-catenin signaling pathway, which is closely connected to KAISO. Hippocampal, frontal cortical, and striatal tissue from C57BL/6j mice with a knockout in the Zbtb33 gene encoding KAISO (ZBTB33-) and wild-type mice (ZBTB33 +) were collected and profiled at different stages of development. Age-dependent and region-specific differences in the mRNA levels of the Fgf9, Fgfr3, c-Myc, FoxG1 genes in the develo** brain of ZBTB33– and ZBTB33+ mice were described and discussed.
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Funding
The experimental part of the work was supported by a grant from the Russian Foundation for Basic Research (no. 18-04-00869 A, brain dissection, RNA isolation and real-time PCR) and budget project no. 0259-2021-0015 (housing and breeding animals) and was carried out using equipment belonging to the Shared Center for Genetic Resources of Laboratory Animals, Federal Research Center of ICG SB RAS, supported by the Ministry of Education and Science of Russia (Unique project identifier RFMEFI62119X0023).
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Statement on the welfare of animals. The treatment of animals was in accordance with the guidelines for the care and use of laboratory animals of the Russian National Center for Genetic Resources of Laboratory Animals in a specific pathogens free vivarium . All procedures involving animals were in accordance with the FELASA ethical standards.
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Illarionova, N.B., Borisova, M.A., Bazhenova, E.Y. et al. Zbtb33 Gene Knockout Changes Transcription of the Fgf9, Fgfr3, c-Myc and FoxG1 Genes in the Develo** Mouse Brain. Mol Biol 55, 363–371 (2021). https://doi.org/10.1134/S0026893321020230
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DOI: https://doi.org/10.1134/S0026893321020230