Abstract
The effect of recombinant glycodelin (GdA) on the number of T-regulatory lymphocytes (Treg) in a culture of activated CD4+ lymphocytes was investigated, while simultaneously assessing the proliferative status of the cells. Recombinant GdA from E. coli and from HEK293 cells were used in the study at concentrations of 0.2; 2 and 10 µg/mL. It was found that only a low concentration (0.2 μg/mL) of recombinant GdA of bacterial origin reduced the number of proliferating CD4+ lymphocytes as well as the number of Treg (CD4+CD25highCD127-/low) in the experimental system in vitro.
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Funding
The study was financially supported by the Russian Foundation for Basic Research, grant no. 19-29-04055 mk, and within the framework of the state task: AAAA-A19-119112290007-7. The equipment of the Center for Collective Use “Investigations of Materials and Substances” of the Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences was used in the work.
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Conflict of interests. The authors declare that they have no conflict of interest.Statement of compliance with standards of research involving humans as subjects. The study was conducted in accordance with the WMA Declaration of Helsinki 2000 and the Protocol to the Council of Europe Convention on Human Rights and Biomedicine 1999. Permission was received from the ethics committee of the IEGM Ural Branch of the Russian Academy of Sciences (IRB00010009) dated March 1, 2022.
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Shardina, K.Y., Timganova, V.P., Bochkova, M.S. et al. The Role of Recombinant Glycodelin in the Differentiation of Regulatory T-Lymphocytes. Dokl Biol Sci 506, 137–140 (2022). https://doi.org/10.1134/S0012496622050131
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DOI: https://doi.org/10.1134/S0012496622050131