Abstract
Antimicrobial peptides are members of the immune system that protect the host from infection. In this study, a potent and structurally novel antimicrobial peptide was isolated and characterized from praying mantis Sphodromantis viridis. This 14-amino acid peptide was purified by RP-HPLC. Tandem mass spectrometry was used for sequencing this peptide, and the results showed that the peptide belongs to the Mastoparan family. The peptide was named Mastoparan-S. Mastoparan-S demonstrated that it has antimicrobial activities against a broad spectrum of microorganisms (Gram-positive and Gram-negative bacteria and fungi), and it was found to be more potent than common antibiotics such as kanamycin. Mastoparan-S showed higher antimicrobial activity against Gram-negative bacteria compared to Gram-positive ones and fungi. The minimum inhibitory concentration (MIC) values of Mastoparan-S are 15.1–28.3 μg/ml for bacterial and 19.3–24.6 μg/ml for fungal pathogens. In addition, this newly described peptide showed low hemolytic activity against human red blood cells. The in vitro cytotoxicity of Mastoparan-S was also evaluated on monolayer of normal human cells (HeLa) by MTT assay, and the results illustrated that Mastoparan-S had significant cytotoxicity at concentrations higher than 40 μg/ml and had no any cytotoxicity at the MIC (−30 μg/ml). The findings of the present study reveal that this newly described peptide can be introduced as an appropriate candidate for treatment of topical infection.
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Published in Russian in Biokhimiya, 2015, Vol. 80, No. 4, pp. 508–516.
Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM14-255, February 15, 2015
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Zare-Zardini, H., Taheri-Kafrani, A., Ordooei, M. et al. Identification and biochemical characterization of a new antibacterial and antifungal peptide derived from the insect Sphodromantis viridis . Biochemistry Moscow 80, 433–440 (2015). https://doi.org/10.1134/S0006297915040069
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DOI: https://doi.org/10.1134/S0006297915040069