SpringerLink
Log in

Immunochromatographic assay for serodiagnosis of tuberculosis using an antigen–colloidal gold conjugate

  • Metrology, Standardization, and Control
  • Published:
Applied Biochemistry and Microbiology Aims and scope Submit manuscript

Abstract

A new immunochromatographic assay was developed for serodiagnosis of tuberculosis based on the interaction of specific anti-Mycobacterium tuberculosis immunoglobulins that are present in the blood serum with a label-antigen conjugate followed by the binding of the resulting complex to the Staphylococcus aureus protein A immobilized on a test strip. Unlike the conventional technique, in which all immunoglobulins that are present in the sample react with a label-immunoglobulin-binding protein conjugate, the new technique eliminates the binding of blood serum immunoglobulins, which are non-specific to Mycobacterium tuberculosis, to the label, and then specific antibodies involved in the labeled complex bind to the immobilized antigen. The new assay was implemented using the recombinant 38-kDa (Rv0934) protein of M. tuberculosis as the antigen and colloidal gold as the label. It was experimentally shown that the new assay allows for an increase in the percentage of the detection of seropositive serum samples with low concentrations of specific antibodies against the causative agent of tuberculosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Thailand)

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BSA:

bovine serum albumin

ICA:

immunochromatographic analysis

CG:

colloidal gold

monoAB:

monoclonal antibodies

OD:

optical density

PBS:

phosphate buffered saline

References

  1. World Health Organization. Global Tuberculosis Report, 2014, WHO, 2014. http://apps.who.int/iris/bitstream/ 10665/137094/9789241564809eng.pdf

    Google Scholar 

  2. Van Kampen, S.C., Ramsay, A.R., Anthony, R.M., and Klatser, P.R., Retooling national TB control programmes (NTPs) with new diagnostics: the NTP perspective, PLoS One, 2010, Vol. 5, p. e11649.

    Article  PubMed Central  PubMed  Google Scholar 

  3. Wallis, R.S., Pai, M., Menzies, D., Doherty, T.M., Walzl, G., Perkins, M.D., and Zumla, A., Biomarkers and diagnostics for tuberculosis: progress, needs, and translation into practice, Lancet, 2010, Vol. 375, pp. 1920–1937.

    Article  CAS  PubMed  Google Scholar 

  4. Dolgov, V.V. and Men’shikov, V.V., Klinicheskaya laboratornaya diagnostika. Natsional’noe rukovodstvo v dvukh tomakh (Clinical Laboratory Diagnostics: National Guide in Two Volumes), Moscow: GEO TARMedia, 2013, Vol. 1.

  5. von Lode, P., Point-of-care immunotesting: approaching the analytical performance of central laboratory methods, J. Clin. Biochem., 2005, Vol. 38, pp. 591–606.

    Article  CAS  Google Scholar 

  6. Wong, R.C. and Tse, H.Y., Lateral Flow Immunoassay, New York: Humana Press, 2009.

    Book  Google Scholar 

  7. Frens, G., Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions, Nat. Phys. Sci., 1973, Vol. 241, pp. 20–22.

    Article  CAS  Google Scholar 

  8. Safenkova, I.V., Zherdev, A.V., and Dzantiev, B.B., Factors influencing the detection limit of the lateralflow sandwich immunoassay: a case study with potato virus X, Anal. Bioanal. Chem., 2012, Vol. 403, pp. 1595–1605.

    Article  CAS  PubMed  Google Scholar 

  9. Bothamley, G.H., Epitope-specific antibody levels in tuberculosis: biomarkers of protection, disease, and response to treatment, Front. Immunol., 2014, Vol. 5, article 243.

    Article  PubMed Central  PubMed  Google Scholar 

  10. Hermanson, G.T., Bioconjugate Techniques, Amsterdam: Academic Press, Elsevier, 2008.

    Google Scholar 

  11. Byzova, N.A., Zvereva, E.A., Zherdev, A.V., Eremin, S.A., and Dzantiev, B.B., Rapid pretreatmentfree immunochromatographic assay of chloramphenicol in milk, Talanta, 2010, Vol. 81, pp. 838–848.

    Article  Google Scholar 

  12. Byzova, N.A., Zvereva, E.A., Zherdev, A.V., Eremin, S.A., Sveshnikov, P.G., and Dzantiev, B.B., Pretreatment-free immunochromatographic assay for the detection of streptomycin and its application to the control of milk and dairy products, Chim. Acta, 2011, Vol. 701, pp. 209–217.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. A.N. Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology”, Russian Academy of Sciences, 119071, Moscow, Russia

    D. V. Sotnikov, A. V. Zherdev & B. B. Dzantiev

  2. Central Tuberculosis Research Institute, Moscow, 107564, Russia

    V. G. Avdienko

Authors
  1. D. V. Sotnikov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. V. Zherdev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. G. Avdienko
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. B. Dzantiev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to D. V. Sotnikov.

Additional information

Original Russian Text © D.V. Sotnikov, A.V. Zherdev, V.G. Avdienko, B.B. Dzantiev, 2015, published in Biotekhnologiya, 2015, No. 1, pp. 76–81.

The article was translated by the authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sotnikov, D.V., Zherdev, A.V., Avdienko, V.G. et al. Immunochromatographic assay for serodiagnosis of tuberculosis using an antigen–colloidal gold conjugate. Appl Biochem Microbiol 51, 834–839 (2015). https://doi.org/10.1134/S0003683815080062

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0003683815080062

Keywords

Search

Navigation