Log in

Characterization of biochemical and proximate composition in rice grains as influenced by germination

  • Original Paper
  • Published:
Cereal Research Communications Aims and scope Submit manuscript

Abstract

The present study was conducted to characterize ten rice (Oryza sativa L.) cultivars viz. IET-23466, Dhan-201, IET-23448, MAS-946, IET-23445, IET-23463, IET-23455, PR-123, PR-115 and IET-23449 based on biochemical (starch, amylose, amylopectin, total sugars, reducing sugars, total protein and amino acids) and proximate composition (moisture, crude protein, crude fibre, ash and fat) before and after germination, with an aim to identify cultivars containing higher health-promoting components after germination. Findings of our study demonstrated that germination significantly influenced the biochemical and proximate components in all the cultivars. Cultivars IET-23463, IET-23466, PR-123 performed better as revealed by higher content of crude protein, ash, crude fibre, reducing sugars and low content of amylose, amylopectin and starch after germination. These cultivars hold great potential after germination and would open up a useful opportunity for the food industries to use them as an ingredient in functional foods. Consumption of these cultivars as ingredients in functional foods after germination would afford greater health benefits to consumers because of increase of fibre, minerals, amino acids and simple sugars after germination.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (France)

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Association of Official Analytical Chemists (1995) Official methods of analysis, 16th edn. AOAC International, Arlington, VA

    Google Scholar 

  • Chinma CE, Anuonye JC, Simon OC, Ohiare RO, Danbaba N (2015) Effect of germination on the physicochemical and antioxidant characteristics of rice flour from three rice varieties from Nigeria. Food Chem 185:454–458. https://doi.org/10.1016/j.foodchem.2015.04.010

    Article  CAS  PubMed  Google Scholar 

  • Cho EE, Chung N (2020) Enhancement of amino acid contents by germination in a Korean weedy rice germplasm. J Crop Sci Biotechnol. https://doi.org/10.1007/s12892-020-00046-5

    Article  Google Scholar 

  • Chompoopong S, Jarungjitaree S, Punbanlaem T, Rungruang T, Chongthamma-kun S, Kettawan A, Taewhowisan T (2016) Neuroprotective effects of germinated brown rice in rotenone-induced Parkinson’s-like disease rats. Neuromol Med 18:334–346

    Article  CAS  Google Scholar 

  • Chrastil J (1987) Improved colorimetric determination of amylose in starches or flours. Carbohydr Res 159:154–158

    Article  CAS  Google Scholar 

  • Chung HJ, Cho A, Lim ST (2014) Utilization of germinated and heat-moisture treated brown rices in sugar-snap cookies. LWT Food Sci Technol 57:260–266

    Article  CAS  Google Scholar 

  • Cornejo F, Caceres PJ, Martínez-Villaluenga C, Rosell CM, Frias J (2015) Effects of germination on the nutritive value and bioactive compounds of brown rice breads. Food Chem 173:298–304

    Article  CAS  Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  • Gong ES, Luo S, Li T, Liu C, Zhang G, Chen J (2017) Phytochemical profiles and antioxidant activity of processed brown rice products. Food Chem 232:67–78

    Article  CAS  Google Scholar 

  • He D, Han C, Yao J, Shen S, Yang P (2011) Constructing the metabolic and regulatory pathways in germinating rice seeds through proteomic approach. Proteomics 11:2693–2713

    Article  CAS  Google Scholar 

  • Ito S, Ishikawa Y (2004) Marketing of value-added rice products in Japan: germinated brown rice and rice bread. FAO Rice Conference 04/CRS.7

  • Kaur H, Gill BS (2020) Comparative evaluation of physicochemical, nutritional and molecular interactions of flours from different cereals as affected by germination duration. Food Meas. https://doi.org/10.1007/s11694-019-00364-5

    Article  Google Scholar 

  • Laxmi G, Chaturvedi N, Richa S (2015) The impact of malting on nutritional composition of foxtail millet, wheat and chickpea. J Nutr Food Sci. https://doi.org/10.4172/2155-9600.1000407

    Article  Google Scholar 

  • Lee YP, Takahashi T (1966) An improved colorimetric determination of amino acid with the use of ninhydrin. Anal Biochem 14:71–77

    Article  CAS  Google Scholar 

  • Li C, Jeong D, Lee JH, Chung HJ (2020) Influence of germination on physicochemical properties of flours from brown rice, oat, sorghum, and millet. Food Sci Biotechnol. https://doi.org/10.1007/s10068-020-00770-2

    Article  PubMed  PubMed Central  Google Scholar 

  • Lowry OH, Rosenbrogh NJ, Farr AL, Randal RJ (1951) Protein measurement with the folin-phenol reagent. J Biol Chem 19:265–275

    Article  Google Scholar 

  • McRae MP (2017) Health benefits of dietary whole grains: An umbrella review of meta analysis. J Chiropract Med 16:10–18

    Article  Google Scholar 

  • Megat Rusydi MR, Noraliza CW, Azrina A, Zulkhairi A (2011) Nutritional changes in germinated legumes and rice varieties. Int Food Res J 18:703–713

    Google Scholar 

  • Moongngarm A, Khomphiphatkul E (2011) Germination time dependence of bioactive compounds and antioxidant activity in germinated rough rice (Oryza sativa L.). Am J Appl Sci 8:15–25

    Article  CAS  Google Scholar 

  • Moongngarm A, Saetung N (2010) Comparison of chemical compositions and bioactive compounds of germinated rough rice and brown rice. Food Chem 122:782–788

    Article  CAS  Google Scholar 

  • Moongngarm A, Moontree T, Deedpinrum P, Padtong K (2014) Functional properties of brown rice Flour as affected by germination. APCBEE Proc 8:41–46

    Article  Google Scholar 

  • Nelson N (1944) A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 153:375–380

    Article  CAS  Google Scholar 

  • Nissar N, Wani SM, Hameed OB, Wani TA, Ahmad M (2017) Influence of paddy (Oryza sativa L.) sprouting on antioxidant activity, nutritional and anti-nutritional properties. Food Meas 11:1844–1850

    Article  Google Scholar 

  • Nonogaki H, Bassel GW, Bewley JD (2010) Germination—still a mystery. Plant Sci 179:574–581

    Article  CAS  Google Scholar 

  • Oghbaei M, Prakash J (2016) Effect of primary processing of cereals and legumes on its nutritional quality: a comprehensive review. Cognt Food Agric 2:136015. https://doi.org/10.1080/23311932.2015.1136015

    Article  CAS  Google Scholar 

  • Pang Y, Ahmed S, Xu Y, Beta T, Zhu Z, Shao Y, Bao J (2018) Bound phenolic compounds and antioxidant properties of whole grain and bran of white, red and black rice. Food Chem 240:212–221

    Article  CAS  Google Scholar 

  • Patil SB, Khan MK (2011) Germinated brown rice as a value added rice product: a review. J Food Sci Technol 48:661–667

    Article  Google Scholar 

  • Peng M, Gao M, Abdel-Aal ESM, Hucl P, Chibbar RN (1999) Separation and characterization of A-and B- type starch granules in wheat endosperm. Cereal Chem 76:375–379

    Article  CAS  Google Scholar 

  • Rodríguez R, Jiménez A, Fernández-Bolaños J, Guillén R, Heredia A (2006) Dietary fibre from vegetable products as source of functional ingredients. Trends Food Sci Technol 17:3–15

    Article  Google Scholar 

  • Saetung N (2006) Study on nutrients and bioactive compounds in germinated rice. Food Technol. Nutr. (Vol. M.S.). Mahasarakham, Thailand: Mahasarakham University, p 101

  • Sharma B, Sharma A, Bhat A, Kishore A (2015) Effect of germination on the chemical composition and nutritive value of maize grain. Bioscan 10:1017–1020

    CAS  Google Scholar 

  • Sibian MS, Saxena DC, Riar CS (2017) Effect of germination on chemical, functional and nutritional characteristics of wheat, brown rice and triticale: a comparative study. J Sci Food Agric 97:4643–4651

    Article  CAS  Google Scholar 

  • Singh AK, Rehal J, Kaur A, Jyot G (2015) Enhancement of attributes of cereals by germination and fermentation: a review. Crit Rev Food Sci Nutr 55:1575–1589

    Article  CAS  Google Scholar 

  • Singh A, Sharma S, Singh B (2017) Effect of germination time and temperature on the functionality and protein solubility of sorghum flour. J Cereal Sci 76:131–139

    Article  CAS  Google Scholar 

  • Singh R, Perez CM, Pascual CG, Juliano BO (1978) Grain size, sucrose level and starch accumulation in develo** rice grain. Phytochem 17:1869–1869

    Article  CAS  Google Scholar 

  • Takeda Y, Takeda C, Mizukami H (1999) Structures of large, medium and small starch granules of barley grain. Carbohydr Polym 38:109–114

    Article  CAS  Google Scholar 

  • Veluppillai S, Nithyanantharajah K, Vasantharuba S, Balakumar S, Arasaratnam V (2009) Biochemical changes associated with germinating rice grains and germination improvement. Rice Sci 16:240–242

    Article  Google Scholar 

  • Wang H, Bouman BAM, Zhao D, Wang C, Moya PF (2002) Aerobic rice in northern China: opportunities and challenges. In: Bouman BAM, Hengsdijk H, Hardy B, Bindraban PS, Tuong TP, Ladha JK (eds) Water-wise rice production. Proc International Workshop on Water-wise Rice Production, 8–11 April 2002, Los Baños, Philippines. International Rice Research Institute, Los Baños, Philippines, pp 143–154

  • World Rice Production 2019. World Rice Production 2019/2020. Available online at: https://www.worldagriculturalproduction.com/crops/rice.aspx/ (accessed May 17, 2019)

  • Wu F, Yang N, Touré A, ** Z, Xu X (2013) Germinated brown rice and its role in human health. Crit Rev Food Sci Nutr 53:451–463

    Article  CAS  Google Scholar 

  • Wunthunyarat W, Seo H, Wang Y (2020) Effects of germination conditions on enzyme activities and starch hydrolysis of long-grain brown rice in relation to flour properties and bread qualities. J Food Sci 85:349–357. https://doi.org/10.1111/1750-3841.15008

    Article  CAS  PubMed  Google Scholar 

  • **a Q, Tao H, Huang P, Wang L, Mei J, Li Y (2017) Minerals in vitro bioaccessibility and changes in textural and structural characteristics of uncooked pre-germinated brown rice influenced by ultra-high pressure. Food Control 71:336–345. https://doi.org/10.1016/j.foodcont.2016.07.018

    Article  CAS  Google Scholar 

  • Yoshida S, Forno DA, Cook JH, Gomes KA (1976) Routine procedure for growing rice plants in culture solution. Laboratory Manual for Physiological Studies of Rice. 3rd ed. Los Banos, Laguna, Philippines: The International Rice Research Institute 61–65

  • Zhang G, Xu Z, Gao Y, Huang X, Yang T (2015) Effects of germination on the nutritional properties, phenolic profiles, and antioxidant activities of buckwheat. J Food Sci 80:H1111–H1119. https://doi.org/10.1111/1750-3841.12830

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Bavita Asthir.

Ethics declarations

Conflict of interest

The authors declare no competing financial interest.

Additional information

Communicated by A. Goyal.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary file1 (DOC 187 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kaur, M., Asthir, B. Characterization of biochemical and proximate composition in rice grains as influenced by germination. CEREAL RESEARCH COMMUNICATIONS 49, 291–299 (2021). https://doi.org/10.1007/s42976-020-00101-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42976-020-00101-5

Keywords

Navigation