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Immunomodulatory effects of different strains of Lactococcus lactis in DSS-induced colitis

  • Veterinary Microbiology - Research Paper
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Abstract

Inflammatory bowel diseases (IBD) are gastrointestinal disorders characterized by a breakdown in intestinal homeostasis by inflammatory immune responses to luminal antigens. Novel strategies for ameliorating IBD have been proposed in many studies using animal models. Our group has demonstrated that administration of Lactococcus lactis NCDO 2118 can improve clinical parameters of colitis induced by oral administration of dextran sulphate sodium (DSS). However, it is not clear whether other strains of L. lactis can yield the same effect. The objective of present study was to analyze the effects of three different L. lactis strains (NCDO2118, IL1403 and MG1363) in the development of DSS-induced colitis in C57BL/6 mice. Acute colitis was induced in C57/BL6 mice by the administration of 2% DSS during 7 consecutive days. Body weight loss and shortening of colon length were observed in DSS-treated mice, and none of L. lactis strains had an impact in these clinical signs of colitis. On the other hand, all strains improved the global macroscopical disease index and prevented goblet cells depletion as well as the increase of intestinal permeability. TNF-α production was reduced in gut mucosa of L. lactis DSS-treated mice indicating a modulation of a critical pro-inflammatory response by all strains tested. However, only L. lactis NCDO2118 and MG1363 induced a higher frequency of CD11c+CD11b−CD103+ tolerogenic dendritic cells in lymphoid organs of mice at steady state. We conclude that all tested strains of L. lactis improved the clinical scores and parameters of colitis, which confirm their anti-inflammatory properties in this model of colitis.

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Acknowledgements

The authors would like to thank Ilda Marçal de Souza and Hermes Oliveira for the excellent care of the animals.

Funding

This study was financially supported by CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnologico), FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais), and PRPq-UFMG (Pró Reitoria de Pesquisa da UFMG), Brazil. Some of the authors are recipients of scholarships (J.L.A., L.L, M.C.C., A.C.G-S.) from CNPq and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), Brazil, and research fellowships (A.M.C.F., D.C.C., V.A., T.U.M.) from CNPq, Brazil.

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Authors and Affiliations

  1. Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil

    Juliana Lima Alves, Luisa Lemos, Nubia Morais Rodrigues, Patrícia A. Vieira Barros, Maria Cecília Campos Canesso, Mauro A. F. Guimarães, Ana Cristina Gomes-Santos & Ana Maria Caetano Faria

  2. Departamento de Genética, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Ecologia E Evolução, Belo Horizonte, MG, Brazil

    Vanessa Bastos Pereira, Anderson Miyoshi & Vasco Ariston Azevedo

  3. Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil

    Denise Carmona Cara

  4. Departamento de Nutrição, Escola de Enfermagem, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil

    Tatiani Uceli Maioli

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  1. Juliana Lima Alves
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Contributions

Experimental procedures were performed by JLA, LL, NMR, MCC, PAVB, VBP and MG. Data analysis was done by JLA, LL, NMR, PAVB, DCC, TUM and ACGS. Bacteria Strains were provided by AM, VAA and VBP. Manuscript writing was prepared by JLA and AMCF. Manuscript revision was performed by JLA and AMCF.

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Correspondence to Juliana Lima Alves or Ana Maria Caetano Faria.

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Alves, J.L., Lemos, L., Rodrigues, N.M. et al. Immunomodulatory effects of different strains of Lactococcus lactis in DSS-induced colitis. Braz J Microbiol 54, 1203–1215 (2023). https://doi.org/10.1007/s42770-023-00928-0

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