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β-Amylase Squirting Cucumber Between Immobilization, Kinetic Modeling and Application for Maltose Syrup Production

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Abstract

A β-amylase extracted from Ecballium elaterium was purified on Q-Sepharose anion exchange chromatography and identified with molecular mass. A successful immobilization by adsorption onto a matrix of titanium dioxide-cellulose acetate butyrate and copolymer of acrylonitrile-acrylamide yielded 32.17%. Thermal stability approved that Ecballium β-amylase retained 89.21% of its initial activity (IA) after 180 min at 70 °C. A promoted catalytic activity was revealed at 70 °C and pH 7.5 with the immobilization procedure. The heterogeneous system based on the process of the substrate conversion degree (α) was studied kinetically via the modified Prout–Tompkins topochemical equation. α increased in accordance with the temperature increase up to 100 min of the reaction time. At 30 °C only 7.6% of optimum conversion degree was lost after 3 h. Power factor (χ) was equal to 1.16. An accomplished application of Ecballium β-amylase proved that it could be a good candidate to produce maltose syrup due to the highest maltose content of 52.9% after 8 h hydrolysis. It will be also very useful during manufacturing processes to minimize starch saccharification time even enzymatic liquefaction costs.

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Authors and Affiliations

  1. Faculty of Sciences, Laboratory of Ecosystems and Biodiversity in Arid Land of Tunisia, Sfax, Tunisia

    Imen Lahmar

  2. Department of Biotechnology, University of Chemical Technology and Metallurgy, Sofia, Bulgaria

    Imen Lahmar, Dessislava Marinkova & Lyubov Yotova

  3. Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria

    Maya Velitchkova

  4. Department of Physical Chemistry, University of Chemical Technology and Metallurgy, Sofia, Bulgaria

    Greta Radeva

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  1. Imen Lahmar
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Correspondence to Imen Lahmar.

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Lahmar, I., Velitchkova, M., Radeva, G. et al. β-Amylase Squirting Cucumber Between Immobilization, Kinetic Modeling and Application for Maltose Syrup Production. Chemistry Africa 7, 1293–1301 (2024). https://doi.org/10.1007/s42250-023-00833-x

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