Abstract
This study reported a novel synthesis of zinc oxide nanoparticles from Mimosa pudica leaves aqueous extract and a chemical method. Synthesis conditions were optimized for the maximum range synthesis of zinc oxide nanoparticles. Mimosa pudica aqueous leaf extract successfully synthesized zinc oxide with a mild bio-stabilizing effect. The resultant powder was characterized using various analytical techniques such as X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), UV–visible spectrometry and Infra-red spectroscopy. Antibacterial activity of the ZnO nanopowder was also carried out. Zinc Oxide nanoparticles (ZnO NPs) from the green route exhibited a slightly lower zone of inhibition of microorganisms than ZnO NPs from the chemical route because of their proximity in size. The SEM report showed that greenly synthesized Zinc Oxide Nanoparticles (GZP) were rod-like while chemically synthesized Zinc Oxide Nanoparticles (CZP) were dumbbell in shape with sizes 101.8 nm and 100.9 nm, respectively.
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References
Ali J, Irshad R, Li B, Tahir K, Ahmad A, Shakeel M, Khan NU, Khan ZUH (2018) Synthesis and characterization of phytochemical fabricated zinc oxide nanoparticles with enhanced antibacterial and catalytic applications. J Photochem Photobiol B 183:349–356
Upadhyaya H, Shome S, Sarma R, Tewari S, Bhattacharya MK, Panda SK (2018) Green synthesis, characterization and antibacterial activity of ZnO nanoparticles. Am J Plant Sci 9:1279–1291
Diallo A, Ngom B, Park E, Maaza M (2015) Green synthesis of ZnO nanoparticles by Aspalathus linearis: structural & optical properties. J Alloy Compd 646:425–430
Jain TK, Morales MA, Sahoo SK, Leslie-Pelecky DL, Labhasetwar V (2005) Iron oxide nanoparticles for sustained delivery of anticancer agents. Mol Pharm 2:194–205
Taimoory SM, Rahdar A, Aliahmad M, Sadeghfar F, Ha**ezhad MR, Jahantigh M, Shahbazi P, Trant JF (2018) The synthesis and characterization of a magnetite nanoparticle with potent antibacterial activity and low mammalian toxicity. J Mol Liq 265:96–104
Hu TY, Frieman M, Wolfram J (2020) Insights from nanomedicine into chloroquine efficacy against COVID-19. Nat Nanotechnol 15:247–249
Das AK, Kim NH, Pradhan D, Hui D, Lee JH (2018) Electrochemical synthesis of palladium (Pd) nanorods: an efficient electrocatalyst for methanol and hydrazine electro-oxidation. Compos B Eng 144:11–18
Mansour H, Bargougui R, Autret-Lambert C, Gadri A, Ammar S (2018) Co-precipitation synthesis and characterization of tin-doped α-Fe2O3 nanoparticles with enhanced photocatalytic activities. J Phys Chem Solids 114:1–7
Duncan TV (2011) Applications of nanotechnology in food packaging and food safety: barrier materials, antimicrobials and sensors. J Colloid Interface Sci 363:1–24
Espitia PJP, Soares NdFF, dos Reis Coimbra JS, de Andrade NJ, Cruz RS, Medeiros EAA (2012) Zinc oxide nanoparticles: synthesis, antimicrobial activity and food packaging applications. Food Bioprocess Technol 5:1447–1464
Luo X, Morrin A, Killard AJ, Smyth MR (2006) Application of nanoparticles in electrochemical sensors and biosensors. Electroanalysis 18:319–326
Amar I, Sharif A, Ali M, Alshareef S, Altohami F, Abdulqadir M, Ahwidi M (2020) Removal of methylene blue from aqueous solutions using nano-magnetic adsorbent based on zinc-doped cobalt ferrite. Chem Methodol 4:1–18
Messaoudi ZA, Lahcene D, Benaissa T, Messaoudi M, Zahraoui B, Belhachemi M, Choukchou-Braham A (2022) Adsorption and photocatalytic degradation of crystal violet dye under sunlight irradiation using natural and modified clays by zinc oxide. Chem Methodol 6:661–676
TaghaviFardood S, Moradnia F, Moradi S, Forootan R, YekkeZare F, Heidari M (2019) Eco-friendly synthesis and characterization of α-Fe2O3 nanoparticles and study of their photocatalytic activity for degradation of Congo red dye. Nanochem Res 4:140–147
Oluwaniyi OO, Adegoke HI, Adesuji ET, Alabi AB, Bodede SO, Labulo AH, Oseghale CO (2016) Biosynthesis of silver nanoparticles using aqueous leaf extract of Thevetia peruviana Juss and its antimicrobial activities. Appl Nanosci 6:903–912
Shittu K, Bankole M, Abdulkareem A, Abubakre O, Ubaka A (2017) Application of gold nanoparticles for improved drug efficiency. Adv Nat Sci Nanosci Nanotechnol 8:035014
Vidya C, Hiremath S, Chandraprabha M, Antonyraj ML, Gopal IV, Jain A, Bansal K (2013) Green synthesis of ZnO nanoparticles by Calotropis gigantea. Int J Curr Eng Technol 1:118–120
Mirzaei H, Darroudi M (2017) Zinc oxide nanoparticles: biological synthesis and biomedical applications. Ceram Int 43:907–914
Kim T, Hyeon T (2013) Applications of inorganic nanoparticles as therapeutic agents. Nanotechnology 25:012001
Sangeetha G, Rajeshwari S, Venckatesh R (2011) Green synthesis of zinc oxide nanoparticles by aloe barbadensis miller leaf extract: structure and optical properties. Mater Res Bull 46:2560–2566
Sozer N, Kokini JL (2009) Nanotechnology and its applications in the food sector. Trends Biotechnol 27:82–89
Raliya R, Tarafdar JC (2013) ZnO nanoparticle biosynthesis and its effect on phosphorous-mobilizing enzyme secretion and gum contents in Clusterbean (Cyamopsis tetragonoloba L.). Agric Res 2:48–57
Sengupta A, Sarkar CK (2015) Introduction to nano: basics to nanoscience and nanotechnology. Springer, New York
Nethavhanani T (2017) Synthesis of zinc oxide nanoparticles by a green process and the investigation of their physical properties
Sabir S, Arshad M, Chaudhari SK (2014) Zinc oxide nanoparticles for revolutionizing agriculture: synthesis and applications. Sci World J 2014:925494
Rosi NL, Mirkin CA (2005) Nanostructures in biodiagnostics. Chem Rev 105:1547–1562
Alavi M, Nokhodchi A (2021) Synthesis and modification of bio-derived antibacterial Ag and ZnO nanoparticles by plants, fungi, and bacteria. Drug Discov Today 8:1953–1962
Gupta K, Chundawat TS (2020) Zinc oxide nanoparticles synthesized using Fusarium oxysporum to enhance bioethanol production from rice-straw. Biomass Bioenerg 143:105840
Momeni SS, Nasrollahzadeh M, Rustaiyan A (2016) Green synthesis of the Cu/ZnO nanoparticles mediated by Euphorbia prolifera leaf extract and investigation of their catalytic activity. J Colloid Interface Sci 472:173–179
Sharma JL, Dhayal V, Sharma RK (2021) White-rot fungus mediated green synthesis of zinc oxide nanoparticles and their impregnation on cellulose to develop environmental friendly antimicrobial fibers. 3 Biotech 11:1–10
Perveen R, Shujaat S, Qureshi Z, Nawaz S, Khan M, Iqbal M (2020) Green versus sol-gel synthesis of ZnO nanoparticles and antimicrobial activity evaluation against panel of pathogens. J Market Res 9:7817–7827
Bala N, Saha S, Chakraborty M, Maiti M, Das S, Basu R, Nandy P (2015) Green synthesis of zinc oxide nanoparticles using Hibiscus subdariffa leaf extract: effect of temperature on synthesis, anti-bacterial activity and anti-diabetic activity. RSC Adv 5:4993–5003
Santhoshkumar J, Kumar SV, Rajeshkumar S (2017) Synthesis of zinc oxide nanoparticles using plant leaf extract against urinary tract infection pathogen. Resour Efficient Technol 3:459–465
Ramesh M, Anbuvannan M, Viruthagiri G (2015) Green synthesis of ZnO nanoparticles using Solanum nigrum leaf extract and their antibacterial activity. Spectrochim Acta Part A Mol Biomol Spectrosc 136:864–870
Liogier AH (1995) Descriptive flora of Puerto Rico and adjacent islands, La Editorial, UPR
Muhammad G, Hussain MA, Jantan I, Bukhari SNA (2016) Mimosa pudica L., a high-value medicinal plant as a source of bioactives for pharmaceuticals. Comp Rev Food Sci Food Saf 15:303–315
Joseph B, George J, Mohan J (2013) Pharmacology and traditional uses of Mimosa pudica. Int J Pharm Sci Drug Res 5:41–44
Saraswat R, Pokharkar R (2012) GCMS studies of Mimosa pudica. Int J Pharm Tech Res 4:93–98
Nwaoga J, Okonkwo W, Unachukwu G (2011) 26th European photovoltaic solar energy conference and exhibition, pp. 406–410
Singh B, Doong R-A, Chauhan DS, Dubey AK (2018) Synthesis and characterization of FeO4/polythiophene hybrid nanocomposites for electroanalytical application. Mater Chem Phys 205:462–469
Azmi L, Singh MK, Akhtar AK (2011) Pharmacological and biological overview on Mimosa pudica Linn. Int J Pharm Life Sci 2:11
Gandhiraja N, Sriram S, Meenaa V, Srilakshmi JK, Sasikumar C, Rajeswari R (2009) Phytochemical screening and antimicrobial activity of the plant extracts of Mimosa pudica L. against selected microbes. Ethnobotanical Leaflets 2009:8
Tamilarasi T, Ananthi T (2012) Phytochemical Analysis and Anti Microbial Activity of Mimosa pudica Linn. Res J Chem Sci ISSN, 2231 (2012) 606X
Harborne A (1998) Phytochemical methods a guide to modern techniques of plant analysis. Springer Science & Business Media, New York
Wu C, Qiao X, Chen J, Wang H, Tan F, Li S (2006) A novel chemical route to prepare ZnO nanoparticles. Mater Lett 60:1828–1832
Getie S, Belay A, Chandra Reddy A, Belay Z (2017) Synthesis and characterizations of zinc oxide nanoparticles for antibacterial applications. J Nanomedic Nanotechnol 8:2
Sen A, Batra A (2012) Evaluation of antimicrobial activity of different solvent extracts of medicinal plant: Melia azedarach L. Int J Curr Pharm Res 4:67–73
Mohammadi FM, Ghasemi N (2018) Influence of temperature and concentration on biosynthesis and characterization of zinc oxide nanoparticles using cherry extract. J Nanostruct Chem 8:93–102
Ahmed S, Ahmad M, Swami BL, Ikram S (2016) A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. J Adv Res 7:17–28
Weng X, Guo M, Luo F, Chen Z (2017) One-step green synthesis of bimetallic Fe/Ni nanoparticles by eucalyptus leaf extract: biomolecules identification, characterization and catalytic activity. Chem Eng J 308:904–911
Mittal AK, Chisti Y, Banerjee UC (2013) Synthesis of metallic nanoparticles using plant extracts. Biotechnol Adv 31:346–356
Sun J, Hu T, Chen C, Zhao D, Yang F, Yang X (2016) Fluorescence immunoassay system via enzyme-enabled in situ synthesis of fluorescent silicon nanoparticles. Anal Chem 88:9789–9795
Yuvakkumar R, Suresh J, Nathanael AJ, Sundrarajan M, Hong S (2014) Novel green synthetic strategy to prepare ZnO nanocrystals using rambutan (Nephelium lappaceum L.) peel extract and its antibacterial applications. Mater Sci Eng C 41:17–27
Senthilkumar N, Nandhakumar E, Priya P, Soni D, Vimalan M, Potheher IV (2017) Synthesis of ZnO nanoparticles using leaf extract of Tectona grandis (L) and their anti- bacterial, anti-arthritic, anti-oxidant and in vitro cytotoxicity activities. New J Chem 41:10347–10356
Khan MF, Ansari AH, Hameedullah M, Ahmad E, Husain FM, Zia Q, Baig U, Zaheer MR, Alam MM, Khan AM (2016) Sol-gel synthesis of thorn-like ZnO nanoparticles endorsing mechanical stirring effect and their antimicrobial activities: potential role as nano-antibiotics. Sci Rep 6:27689
Kour J, Khanna K, Sharma P, Arora P, Dhiman S, Kaur R, Sharma A, Ohri P, Bhardwaj R (2021) Variability, behaviour and impact of nanoparticles in the environment, plant responses to nanomaterials. Springer, New York, pp 315–328
Zare E, Pourseyedi S, Khatami M, Darezereshki E (2017) Simple biosynthesis of zinc oxide nanoparticles using nature’s source, and it’s in vitro bio-activity. J Mol Struct 1146:96–103
Singh A, Singh N, Hussain I, Singh H, Yadav V, Singh S (2016) Green synthesis of nano zinc oxide and evaluation of its impact on germination and metabolic activity of Solanum lycopersicum. J Biotechnol 233:84–94
Wahab R, Kim Y-S, Hwang I, Shin H-S (2009) A non-aqueous synthesis, characterization of zinc oxide nanoparticles and their interaction with DNA. Synth Met 159:2443–2452
Wahab R, Ansari S, Seo H-K, Kim YS, Suh E-K, Shin H-S (2009) Low temperature synthesis and characterization of rosette-like nanostructures of ZnO using solution process. Solid State Sci 11:439–443
Jalal R, Goharshadi EK, Abareshi M, Moosavi M, Yousefi A, Nancarrow P (2010) ZnO nanofluids: green synthesis, characterization, and antibacterial activity. Mater Chem Phys 121:198–201
Jafarirad S, Mehrabi M, Divband B, Kosari-Nasab M (2016) Biofabrication of zinc oxide nanoparticles using fruit extract of Rosa canina and their toxic potential against bacteria: a mechanistic approach. Mater Sci Eng C 59:296–302
Talam S, Karumuri SR, Gunnam N (2012) Synthesis, characterization, and spectroscopic properties of ZnO nanoparticles. ISRN Nanotechnol 2012:1–6
Zhou J, Zhao F, Wang Y, Zhang Y, Yang L (2007) Size-controlled synthesis of ZnO nanoparticles and their photoluminescence properties. J Lumin 122:195–197
Jamdagni P, Khatri P, Rana J (2018) Green synthesis of zinc oxide nanoparticles using flower extract of Nyctanthes arbor-tristis and their antifungal activity. J King Saud Univ Sci 30:168–175
Khoshhesab ZM, Sarfaraz M, Asadabad MA (2011) Preparation of ZnO nanostructures by chemical precipitation method. Synth React Inorg Met-Org Nano-Met Chem 41:814–819
Jamdagni P, Rana JS, Khatri P, Nehra K (2018) Comparative account of antifungal activity of green and chemically synthesized zinc oxide nanoparticles in combination with agricultural fungicides. Int J Nano Dimens 9:198–208
Zhang L, Ding Y, Povey M, York D (2008) ZnO nanofluids—a potential antibacterial agent. Prog Nat Sci 18:939–944
He C, Sasaki T, Shimizu Y, Koshizaki N (2008) Synthesis of ZnO nanoparticles using nanosecond pulsed laser ablation in aqueous media and their self-assembly towards spindle- like ZnO aggregates. Appl Surf Sci 254:2196–2202
Ramesh P, Rajendran A, Meenakshisundaram M (2014) Green syntheis of zinc oxide nanoparticles using flower extract cassia auriculata. J Nanosci Nanotechnol 2:41–45
Ogunyemi SO, Abdallah Y, Zhang M, Fouad H, Hong X, Ibrahim E, Masum MMI, Hossain A, Mo J, Li B (2019) Green synthesis of zinc oxide nanoparticles using different plant extracts and their antibacterial activity against Xanthomonas oryzae pv. oryzae. Artif Cells Nanomed Biotechnol 47:341–352
Dobrucka R, Długaszewska J (2016) Biosynthesis and antibacterial activity of ZnO nanoparticles using Trifolium pratense flower extract. Saudi J Biol Sci 23:517–523
Yang C, Tang L, Li Q, Bai A, Wang Y, Yu Y (2015) Preparation of monodisperse colloidal ZnO nanoparticles and their optical properties. NANO 10:150322185915002
Kundu D, Hazra C, Chatterjee A, Chaudhari A, Mishra S (2014) Extracellular biosynthesis of zinc oxide nanoparticles using Rhodococcus pyridinivorans NT2: multifunctional textile finishing, biosafety evaluation and in vitro drug delivery in colon carcinoma. J Photochem Photobiol B 140:194–204
Ukwueze C, Okogwu O, Ebem E, Nwonumara G, Nwodo J (2019) Evaluation of the influence of geographical location on phytochemical composition of Moringa oleifera seeds. World Appl Sci J 37:196–201
Parvathy P, Murali V, Devi VM, Murugan M, Jmaes JJ (2020) ICP-MS assisted heavy metal analysis, phytochemical, proximate and antioxidant activities of Mimosa pudica L. Mater Today Proc 45:2265–2269
Kühn KP, Chaberny IF, Massholder K, Stickler M, Benz VW, Sonntag H-G, Erdinger L (2003) Disinfection of surfaces by photocatalytic oxidation with titanium dioxide and UVA light. Chemosphere 53:71–77
Ohira T, Yamamoto O, Iida Y, Nakagawa Z-E (2008) Antibacterial activity of ZnO powder with crystallographic orientation. J Mater Sci Mater Med 19:1407–1412
Sawai J, Shoji S, Igarashi H, Hashimoto A, Kokugan T, Shimizu M, Kojima H (1998) Hydrogen peroxide as an antibacterial factor in zinc oxide powder slurry. J Ferment Bioeng 86:521–522
Kanwal A, Qaseem S, Naeem M, Ali SR, Shaffique M, Maqbool M (2019) Size-dependent inhibition of bacterial growth by chemically engineered spherical ZnO nanoparticles. J Biol Phys 45:147–159
**ng Y, Li X, Zhang L, Xu Q, Che Z, Li W, Bai Y, Li K (2012) Effect of TiO2 nanoparticles on the antibacterial and physical properties of polyethylene-based film. Prog Org Coat 73:219–224
Janaki AC, Sailatha E, Gunasekaran S (2015) Synthesis, characteristics and antimicrobial activity of ZnO nanoparticles. Spectrochim Acta Part A Mol Biomol Spectrosc 144:17–22
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Adegoke, H.I., Gbenga, A.A. Bio-Assisted Synthesis of Zinc Oxide Nanoparticles from Mimosa pudica Aqueous Leave Extract: Structure and Antibacterial Activity. Chemistry Africa 6, 1283–1296 (2023). https://doi.org/10.1007/s42250-022-00581-4
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DOI: https://doi.org/10.1007/s42250-022-00581-4