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Customizable design of multiple-biomolecule delivery platform for enhanced osteogenic responses via ‘tailored assembly system’

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Abstract

Porous titanium (Ti) scaffolds have been extensively utilized as bone substitute scaffolds due to their superior biocompatibility and excellent mechanical properties. However, naturally formed TiO2 on the surface limits fast osseointegration. Different biomolecules have been widely utilized to overcome this issue; however, homogeneous porous Ti scaffolds could not simultaneously deliver multiple biomolecules that have different release behaviors. In this study, functionally graded porous Ti scaffolds (FGPTs) with dense inner and porous outer parts were fabricated using a two-body combination and densification procedure. FGPTs with growth factor (BMP-2) and antibiotics (TCH) exhibited suitable mechanical properties as bone substituting material and presented good structural stability. The release of BMP-2 was considerably prolonged, whereas the release of TCH was comparable to that of homogenous porous titanium scaffolds (control group). The osteogenic differentiation obtained using FGPTs was maintained due to the prolonged release of BMP-2. The antimicrobial properties of these scaffolds were verified using S. aureus in terms of prior release time. In addition, various candidates for graded porous Ti scaffolds with altered pore characteristics were presented.

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Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (Nos. 2021R1I1A1A01043176 and 2021R1A2C1091301), the framework of international cooperation program managed by the National Research Foundation of Korea (No. 2021K2A9A2A06037540), Korean Fund for Regenerative Medicine funded by Ministry of Science and ICT, and Ministry of Health and Welfare (No. 2021M3E5E5096420, Republic of Korea), Korea Medical Device Development Fund grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health & Welfare, Republic of Korea, the Ministry of Food and Drug Safety) (Project Number: 202011B29), and the GRRC program of the Gyeo nggi Province (Grant Number GRRC-KPU2021-A01, Multi-material Machining Innovative Technology Research Center).

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Authors and Affiliations

  1. Department of Biomedical-Chemical Engineering, Catholic University of Korea, Bucheon, 14662, Republic of Korea

    Hyun Lee, Ginam Han, Yuhyeon Na & Hyun-Do Jung

  2. Department of Biotechnology, The Catholic University of Korea, Bucheon, 14662, Republic of Korea

    Hyun Lee, Ginam Han, Yuhyeon Na & Hyun-Do Jung

  3. Department of Materials Science and Engineering, Seoul National University, Seoul, 08826, Republic of Korea

    Min-Kyu Lee & Hyoun-Ee Kim

  4. Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL, 60208, USA

    Min-Kyu Lee

  5. Biomedical Implant Convergence Research Center, Advanced Institutes of Convergence Technology, Suwon, 443-270, Republic of Korea

    Hyoun-Ee Kim

  6. School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore, 637457, Singapore

    Juha Song

  7. Department of Advanced Materials Engineering, Korea Polytechnic University, Siheung-si, Gyeonggi-do, 15073, Republic of Korea

    Chang-Bun Yoon

  8. Surface R&D Group, Korea Institute of Industrial Technology, Incheon, 21999, Republic of Korea

    SeKwon Oh

  9. Department of Materials Science and Engineering, Chosun University, Gwangju, 61452, Republic of Korea

    Tae-Sik Jang

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  1. Hyun Lee
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Contributions

HL contributed to conceptualization, investigation, validation, methodology, and writing—original draft. MKL contributed to investigation, validation, and methodology. GH contributed to validation and visualization. HEK contributed to project administration and supervision. JS and YN contributed to conceptualization and methodology. SO and CBY contributed to writing—revised draft. TSJ contributed to conceptualization, methodology, and writing—review and editing. HDJ contributed to conceptualization, methodology, writing—original draft, and writing—review and editing.

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Correspondence to Tae-Sik Jang or Hyun-Do Jung.

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Lee, H., Lee, MK., Han, G. et al. Customizable design of multiple-biomolecule delivery platform for enhanced osteogenic responses via ‘tailored assembly system’. Bio-des. Manuf. 5, 451–464 (2022). https://doi.org/10.1007/s42242-022-00190-7

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