Abstract
The present experiment was conducted to assess the uptake of green synthesized silver nanoparticles (GSAgNPs) in hydroponically grown wheat seedlings. The tendency to apply plant-based silver nanoparticles to crops such as wheat is gaining importance because of their role in enhancing growth and yield. The green synthesis of silver nanoparticles was carried out through reducing silver salt (AgNO3) with Moringa oleifera plant green leaves extract. Silver nanoparticles were characterized through UV–visible spectroscopy, scanning electron microscopy, and zeta analyzer. Wheat seedlings were grown in different concentrations of GSAgNPs (0, 25, 50, 75, 100 mg/l) under a controlled environment. Thin root cross sections (1 to 2 µm) were developed from 1-week-old roots and analyzed under a high-resolution microscope with a PC system to check the presence of GSAgNPs. The presence of GSAgNPs was observed in all treated wheat seedlings as compared with control, where no particle-like entities existed. Nanoparticle uptake and accumulation were more in plants treated with high concentrations (75 and 100 mg/l). Overall horizontal and vertical movement of nanoparticles was revealed in all treated wheat seedlings. This concludes that GSAgNPs have the potential to enter plants and to move to specific locations through different tissues. In the future, this study’s findings could be very helpful in determining the potential deliverance and retention of plant-based nanoparticles in the fields of agriculture and medical sciences.
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Acknowledgements
We are very grateful to Dr. Noshin Ilyas, Assistant Professor of Botany, for providing kind guidance during the microscopy analysis. We are also very grateful to the Higher Education Commission (HEC) of Pakistan for its support in the successful completion of this experimental work.
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Iqbal, M., Raja, N.I., Mashwani, ZUR. et al. Assessment of Green Synthesized Silver Nanoparticles in Wheat Seedlings at the Anatomical Level in Relation to Their Uptake, Translocation, and Accumulation. Iran J Sci Technol Trans Sci 43, 1551–1561 (2019). https://doi.org/10.1007/s40995-018-0639-0
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DOI: https://doi.org/10.1007/s40995-018-0639-0