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Treatment of Periodontal Bone Defects with Stem Cells from Inflammatory Dental Pulp Tissues in Miniature Swine

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

Containing a certain proportion of mesenchymal stem cells, inflammatory dental tissue showed great tissue regeneration potential in recent years. However, whether it is applicable to promote tissue regeneration in vivo remains to be elucidated. Therefore, we evaluated the feasibility of stem cells from inflammatory dental pulp tissues (DPSCs-IPs) to reconstruct periodontal defects in miniature pigs.

Methods:

The autologous pig DPSCs-IPs were first cultured, appraised and loaded onto β-tricalcium phosphate (β-TCP). The compounds were then engrafted into an artificially-created periodontal defect. Three months later, the extent of periodontal regeneration was evaluated. Clinical examination, radiological examination and immunohistochemical staining were used to assess periodontal regeneration.

Results:

The data collectively showed that DPSCs-IPs from miniature pigs expressed moderate to high levels of STRO-1 and CD146 as well as low levels of CD34 and CD45. DPSCs-IPs have osteogentic, adipogenic and chondrogenic differentiation abilities. DPSCs-IPs were engrafted onto β-TCP and regenerated bone to repair periodontal defects by 3 months’ post-surgical reconstruction.

Conclusion:

Autologous DPSCs-IPs may be a feasible means of periodontal regeneration in miniature pigs.

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Acknowledgements

This research was funded by Shaanxi Provincial Science and Technology Innovation Project co-ordination of Resources Oriented Industries of Key Technologies Project (Grant No. 2011KTCL03-24 for A.L.) and Scientific and Technological Innovation Project in **’an (Grant No. SF1421 for T.L.).

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Authors and Affiliations

  1. Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, **’an Jiaotong University, **’an, Shaanxi, China

    Ye Li, Tong Li & Ang Li

  2. Department of Periodontology, College of Stomatology, **’an Jiaotong University, ** Wu Road No. 98, **’an, 710004, Shaanxi, China

    Ye Li, ** Nan & Ang Li

  3. Department of Orthodontics, College of Stomatology, **’an Jiaotong University, ** Wu Road No. 98, **’an, 710004, Shaanxi, China

    Tian-Yu Zhong & Tong Li

Authors
  1. Ye Li
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  2. ** Nan
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  3. Tian-Yu Zhong
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  4. Tong Li
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  5. Ang Li
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Correspondence to Tong Li.

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The authors declare that they have no conflict of interest.

Ethical statement

The study has been independently reviewed and approved by the ethical board of **’an Jiaotong University College of Medicine for Experimental Animals (No. 2017036).

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Li, Y., Nan, X., Zhong, TY. et al. Treatment of Periodontal Bone Defects with Stem Cells from Inflammatory Dental Pulp Tissues in Miniature Swine. Tissue Eng Regen Med 16, 191–200 (2019). https://doi.org/10.1007/s13770-018-00175-7

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  • DOI: https://doi.org/10.1007/s13770-018-00175-7

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