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Three-dimensional culture of mesenchymal stem cells

  • Review Article
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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Mesenchymal stem cells (MSCs) are partially defined by their ability to differentiate into different tissues in vitro–including the bone, cartilage, and adipose tissue, as well as their ability to secrete bioactive factors in response to local microenvironmental cues. A significant amount of information exists on the biochemical, metabolic, and feedback mechanisms associated with MSC response, but the clinical applications of MSCs have been limited by changes in their in vitro capabilities that occur upon transplantation into the body. These inconsistencies occur because most preclinical studies on MSCs have been conducted in two-dimensional cell culture plates rather than in a three-dimensional (3D) cell culture system, which is more physiologically similar to the extracellular matrix found in the body. Thus, MSCs form thin monolayer devoid of vertical cell-cell interaction that prevents MSCs from colonizing. In this article, we review the history and characteristics of MSCs, related developments in 3D cell culture research, and some MSC-based clinical trials of MSCs in order to give a clear understanding of 3D cell culture systems in the field of tissue engineering with respect to MSC biology.

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Authors and Affiliations

  1. Convergence Stem Cell Research Center, Medical Research Institute, School of Medicine, Pusan National University, Yangsan, Korea

    Jong Kyu Hong, Jisoo Yun, Hyunyun Kim & Sang-Mo Kwon

  2. Laboratory for Vascular Medicine and Stem Cell Biology, Medical Research Institute, Department of Physiology, School of Medicine, Pusan National University, Yangsan, Korea

    Jong Kyu Hong, Jisoo Yun, Hyunyun Kim & Sang-Mo Kwon

  3. Immunoregulatory Therapeutics Group in Brain Busan 21 Project, Department of Physiology, Pusan National University School of Medicine, Yangsan, Korea

    Sang-Mo Kwon

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  1. Jong Kyu Hong
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  2. Jisoo Yun
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Hong, J.K., Yun, J., Kim, H. et al. Three-dimensional culture of mesenchymal stem cells. Tissue Eng Regen Med 12, 211–221 (2015). https://doi.org/10.1007/s13770-015-0005-7

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