Abstract
The ASR gene is found in various plant species. Although ASR is also expressed in sink tissues, most functional studies on this gene have been performed in source leaves. Here, we report that OsASR1 is involved in the abscisic acid (ABA) response via regulation of sugar levels in the roots. Overexpression of OsASR1 altered root sugar content by increasing fructose levels and decreasing sucrose levels. Moreover, the transcript levels of sugar transporters and sucrose metabolism-related genes were altered in the roots of OsASR1-OX lines. Upon ABA treatment, an additional increase in the glucose and fructose levels was observed in the roots of OsASR1-OX lines compared with ABA-treated NT controls and untreated OsASR1-OX lines. Compared with NT control, the root growth of OsASR1-OX lines was more strongly inhibited by 10 % glucose. Our results suggest that OsASR1 plays an important role in the ABA response and sugar signaling in the roots.
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References
Arenhart RA, Lima JC, Pedron M, Carvalho FE, Silveira JA, Rosa SB, Caverzan A, Andrade CM, Schunemann M, Margis R, Margis-Pinheiro M (2013) Involvement of ASR genes in aluminium tolerance mechanisms in rice. Plant Cell Environ 36:52–67
Boyer JS (1982) Plant productivity and environment. Science 218:443–448
Cakir B, Agasse A, Gaillard C, Saumonneau A, Delrot S, Atanassova R (2003) A grape ASR protein involved in sugar and abscisic acid signaling. Plant Cell 15:2165–2180
Carrari F, Fernie AR, Iusem ND (2004) Heard it through the grapevine? ABA and sugar cross-talk: the ASR story. Trends Plant Sci 9:57–59
Dai JR, Liu B, Feng DR, Liu HY, He YM, Qi KB, Wang HB, Wang JF (2011) MpAsr encodes an intrinsically unstructured protein and enhances osmotic tolerance in transgenic Arabidopsis. Plant Cell Rep 30:1219–1230
Dominguez PG, Frankel N, Mazuch J, Balbo I, Iusem ND, Fernie AR, Carrari F (2013) ASR1 mediates glucose-hormone crosstalk by affecting sugar trafficking in tobacco plants. Plant Physiol 161:1486–1500
Frankel N, Nunes-Nesi A, Balbo I, Mazuch J, Centeno D, Iusem ND, Fernie AR, Carrari F (2007) ci21A/Asr1 expression influences glucose accumulation in potato tubers. Plant Mol Biol 63:719–730
Gupta AK, Kaur N (2005) Sugar signalling and gene expression in relation to carbohydrate metabolism under abiotic stresses in plants. J Biosci 30:761–776
Harmer SL, Hogenesch JB, Straume M, Chang HS, Han B, Zhu T, Wang X, Kreps JA, Kay SA (2000) Orchestrated transcription of key pathways in arabidopsis by the circadian clock. Science 290:2110–2113
Hirasawa T (1999) Physiological characterization of rice plant for tolerance of water deficit. In: Ito OO, Toole JC, Hardy B (eds) Genetic improvement of rice for water-limited environments. International Rice Research Institute, Philippines, pp 89–98
Hsu YF, Yu SC, Yang CY, Wang CS (2011) Lily ASR protein-conferred cold and freezing resistance in Arabidopsis. Plant Physiol Biochem 49:937–945
Hu W, Huang C, Deng X, Zhou S, Chen L, Li Y, Wang C, Ma Z, Yuan Q, Wang Y, Cai R, Liang X, Yang G, He G (2013) TaASR1, a transcription factor gene in wheat, confers drought stress tolerance in transgenic tobacco. Plant Cell Environ 36:1449–1464
Iusem ND, Bartholomew DM, Hitz WD, Scolnik PA (1993) Tomato (Lycopersicon esculentum) transcript induced by water deficit and ripening. Plant Physiol 102:1353–1354
Jelitto T, Sonnewald U, Willmitzer L, Hajirezaei M, Stitt M (1992) Inorganic pyrophosphate content and metabolites in potato and tobacco plants expressing E. coli pyrophosphatase in their cytosol. Planta 188:238–244
Joo J, Lee YH, Choi DH, Cheong JJ, Kim YK, Song SI (2013a) Rice ASR1 has function in abiotic stress tolerance during early growth stages of rice. J Korean Soc Appl Biol Chem 56:349–352
Joo J, Lee YH, Kim YK, Nahm BH, Song SI (2013b) Abiotic stress responsive rice ASR1 and ASR3 exhibit different tissue-dependent sugar and hormone-sensitivities. Mol Cells 35:421–435
Joo J, Choi HJ, Lee YH, Lee S, Lee CH, Kim CH, Cheong JJ, Choi YD, Song SI (2014) Over-expression of BvMTSH, a fusion gene for maltooligosyltrehalose synthase and maltooligosyltrehalose trehalohydrolase, enhances drought tolerance in transgenic rice. BMB Rep 47:27–32
Kalifa Y, Perlson E, Gilad A, Konrad Z, Scolnik PA, Bar-Zvi D (2004) Over-expression of the water and salt stress-regulated Asr1 gene confers an increased salt tolerance. Plant Cell Environ 27:1459–1468
Kim SJ, Lee SC, Hong SK, An K, An G, Kim SR (2009) Ectopic expression of a cold-responsive OsAsr1 cDNA gives enhanced cold tolerance in transgenic rice plants. Mol Cells 27:449–458
Liu HY, Dai JR, Feng DR, Liu B, Wang HB, Wang JF (2010) Characterization of a novel plantain Asr gene, MpAsr, that is regulated in response to infection of Fusarium oxysporum f. sp. cubense and abiotic stresses. J Integr Plant Biol 52:15–323
Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y, Kamiya A, Nakajima M, Enju A, Sakurai T, Satou M, Akiyama K, Taji T, Yamaguchi-Shinozaki K, Carninci P, Kawai J, Hayashizaki Y, Shinozaki K (2002) Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. Plant J 31:279–292
Shkolnik D, Bar-Zvi D (2008) Tomato ASR1 abrogates the response to abscisic acid and glucose in Arabidopsis by competing with ABI4 for DNA binding. Plant Biotechnol J 6:368–378
Shu L, Lou Q, Ma C, Ding W, Zhou J, Wu J, Feng F, Lu X, Luo L, Xu G, Mei H (2011) Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought. Proteomics 11:4122–4138
Siahpoosh MR, Sanchez DH, Schlereth A, Scofield GN, Furbank RT, van Dongen JT, Kopka J (2012) Modification of OsSUT1 gene expression modulates the salt response of rice Oryza sativa cv. Taipei 309. Plant Sci 182:101–111
Stitt M, Lilley RM, Gerhardt R, Heldt HW (1989) Metabolite levels in specific cells and subcellular compartments of plant leaves. Methods Enzymol 174:518–522
Sun Y, Reinders A, LaFleur KR, Mori T, Ward JM (2010) Transport activity of rice sucrose transporters OsSUT1 and OsSUT5. Plant Cell Physiol 51:114–122
Sun A, Dai Y, Zhang X, Li C, Meng K, Xu H, Wei X, **ao G, Ouwerkerk PB, Wang M, Zhu Z (2011) A transgenic study on affecting potato tuber yield by expressing the rice sucrose transporter genes OsSUT5Z and OsSUT2M. J Integr Plant Biol 53:86–595
Tabaeizadeh Z (1998) Drought-induced responses in plant cells. Int Rev Cytol 182:193–247
Virlouvet L, Jacquemot MP, Gerentes D, Corti H, Bouton S, Gilard F, Valot B, Trouverie J, Tcherkez G, Falque M, Damerval C, Rogowsky P, Perez P, Noctor G, Zivy M, Coursol S (2011) The ZmASR1 protein influences branched-chain amino acid biosynthesis and maintains kernel yield in maize under water-limited conditions. Plant Physiol 157:917–936
Wingler A, Roitsch T (2008) Metabolic regulation of leaf senescence: interactions of sugar signalling with biotic and abiotic stress responses. Plant Biol (Stuttg) 10(Suppl 1):50–62
Zhang H, Liang W, Yang X, Luo X, Jiang N, Ma H, Zhang D (2010) Carbon starved anther encodes a MYB domain protein that regulates sugar partitioning required for rice pollen development. Plant Cell 22:672–689
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This work was supported by the Technology Development Program for Life Industry through the Korea Institute of Planning and Evaluation for Technology of Food, Agriculture, Forestry and Fisheries (Grant number 111076-5).
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Joo, J., Song, S.I. Rice ASR1 regulates sugar levels and participates in sugar signaling in roots. J Korean Soc Appl Biol Chem 58, 161–167 (2015). https://doi.org/10.1007/s13765-015-0008-z
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DOI: https://doi.org/10.1007/s13765-015-0008-z