Abstract
Insecticidal gene silencing by RNA interference (RNAi) involves a post-transcriptional mechanism with great potential for insect control. Here, we aim to summarize the progress on RNAi research toward control of insect pests in the Neotropical region and discuss factors determining its efficacy and prospects for pest management. We include an overview of the available RNAi information for Neotropical pests in the Lepidoptera, Coleoptera, Diptera, and Hemiptera orders. Emphasis is put on significant findings in the use of RNAi against relevant Neotropical pests, including diamondback moth (Plutella xylostella L.), Asian citrus psyllid (Diaphorina citri Kuwayama), and the cotton boll weevil (Anthonomus grandis Boheman). We also examine the main factors involved in insecticidal RNAi efficiency and major advances to improve screening of lethal genes, formulation, and delivery. Few studies detail resistance mechanisms to RNAi, demonstrating a need for more research. Advances in formulation, delivery, and resistance management tools for insecticidal RNAi in the Neotropics can provide a basis for efficient field application.
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Author Contribution Statement
NPD, DC, GS, and MZ contributed to the conception of the manuscript. NPD and DC wrote the first draft. NPD, DC, EAS, GS, JLJF, and SM wrote sections of the manuscript. GS, DEN, MZ, and JLJF revised and edited the manuscript. All authors read, contributed critically to the drafts, and approved the final version.
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The authors thank the Coordination of Improvement of Higher Education Personnel (CAPES) and the National Council for Scientific and Technological Development (CNPq) for the doctoral scholarships for NPD and DC in Brazil.
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Dias, N.P., Cagliari, D., dos Santos, E.A. et al. Insecticidal Gene Silencing by RNAi in the Neotropical Region. Neotrop Entomol 49, 1–11 (2020). https://doi.org/10.1007/s13744-019-00722-4
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DOI: https://doi.org/10.1007/s13744-019-00722-4