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Map** novel sources of leaf rust and stripe rust resistance introgressed from Triticum dicoccoides in cultivated tetraploid wheat background

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Abstract

Wild emmer wheat, Triticum dicoccoides, the progenitor of modern tetraploid and hexaploid wheats, is an important resource for new variability for disease resistance genes. T. dicoccoides accession pau4656 showed resistance against prevailing leaf rust and stripe rust races in India and was used for develo** stable introgression lines (IL) in T. durum cv Bijaga yellow and named as IL pau16068. F5 Recombinant inbred lines (F5 RILs) were developed by crossing IL pau16068 with T. durum cultivar PBW114 and RIL population was screened against highly virulent Pt and Pst pathotypes at the seedling and adult plant stages. Inheritance analyses revealed that population segregated for two genes for all stage resistance (ASR) against leaf rust, one ASR gene against stripe rust and three adult plant resistance (APR) genes for stripe rust resistance. For map** these genes a set of 483 SSR marker was used for bulked segregant analysis. The markers showing diagnostic polymorphism in the resistant and susceptible bulks were amplified on all RILs. Single marker analysis placed all stage leaf rust resistance genes on chromosome 6A and 2A linked to the SSR markers Xwmc256 and Wpaus268, respectively. Likewise one all stage stripe rust resistance gene were mapped on long arm of chromosome 6A linked to markers 6AL-5833645 and 6AL-5824654 and two APR genes mapped on chromosomes 2A and 2B close to the SSR marker Wpaus268 and Xbarc70, respectively. The current study identified valuable leaf rust and stripe rust resistance genes effective against multiple rust races for deployment in the wheat breeding programme.

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Abbreviations

Pst :

Puccinia striiformis

Pt :

Puccinia triticina

SSR:

Simple sequence repeats

IL:

Introgression line

QTL:

Quantitative trait loci

RIL:

Recombinant inbred line

BSA:

Bulked segregant analysis

IT:

Infection type

ASR:

All stage resistance

APR:

Adult plant resistance

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Acknowledgements

Financial support provided by Department of Biotechnology. Ministry of Science and Technology, GOI is gratefully acknowledged. First authors acknowledge the support provided by The World Academy of Sciences and DBT in the form of TWAS_DBT Post-Doctoral Fellowship. The provision of rust cultures by the Directorate of Wheat Research Regional Research Station, Shimla is thankfully acknowledged.

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Authors and Affiliations

  1. School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141004, India

    Ahmed Fawzy Elkot, Rohtas Singh, Satinder Kaur, Jaspal Kaur & Parveen Chhuneja

  2. Wheat Research Department, Agricultural Research Center, Field Crops Research Institute, Giza, 12619, Egypt

    Ahmed Fawzy Elkot

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  1. Ahmed Fawzy Elkot
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  3. Satinder Kaur
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Contributions

AFE—Phenoty** and genoty** of the population; RS—genoty** of the population; SK—material development and rust phenoty**; JK—screening against stripe rust races PC—study concept, material development and manuscript draft.

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Correspondence to Parveen Chhuneja.

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13562_2020_598_MOESM1_ESM.xlsx

Supplementary table 1: List of A and B chromosome specific SSR and KASP primers designed from wheat genome sequence. (XLSX 13 kb)

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Elkot, A.F., Singh, R., Kaur, S. et al. Map** novel sources of leaf rust and stripe rust resistance introgressed from Triticum dicoccoides in cultivated tetraploid wheat background. J. Plant Biochem. Biotechnol. 30, 336–342 (2021). https://doi.org/10.1007/s13562-020-00598-1

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