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Sustainable biosynthesis of β-carotene utilizing sugarcane bagasse: depiction and biotechnological implications

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Abstract

This study focused on optimizing β-carotene production by Nesterenkonia sp. K-15–9-6 through submerged fermentation (SmF), which aims majorly for cost reduction and eco-friendliness. A total of eight different agro-food wastes were explored for designing the production medium among which sugarcane bagasse showed prominent amounts of β-carotene. Upon optimization of various factors, it was observed that the maximum pigment (820µg/ml) was produced utilizing 3g (w/v) sugarcane bagasse, 1% (v/v) glycerol, 2.5% (w/v) NaCl, 0.5% (w/v) peptone, and 0.5% (w/v) dextrose (inducer). The solvent extraction method suggests that methanol proved to be the best solvent for pigment extraction. The β-carotene product confirmation was done via absorption maxima, thin layer chromatography (TLC), high-performance liquid chromatography (HPTLC), Fourier transformation infrared spectroscopy (FTIR), and mass spectrophotometry (MS). Phyto-toxicity assay of pigment on Sorghum (Sorghum bicolor), Fenugreek (Trigonella foenum-graecum), and Fennel seed (Foeniculum vulgare) confirmed the safety as well as plant growth-promoting ability. The β-carotene has varied applications such as antimicrobial, antioxidant, textile dyeing, food additives, cosmetics, and candles. The findings emphasize the viability and sustainability of utilizing sugarcane bagasse, for biosynthesis of β-carotene in an economic way.

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Acknowledgements

All the authors kindly acknowledge the infrastructural support provided by the DST-FIST-sponsored Department of Microbiology & Biotechnology, University School of Sciences, Gujarat University, Ahmedabad. The experimental support from the Department of Zoology & Biomedical Technology, Gujarat University, Ahmedabad, and the Indian Institute of Technology, Mandi (IIT-Mandi), India is generously acknowledged.

Funding

Author PSA kindly acknowledges the SHODH-Scheme of Development High-Quality Research fellowship by the Department of Higher Education, Government of Gujarat Ref No. 201901380034.

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Authors and Affiliations

  1. Department of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad, 380009, India

    Shivani Yagnik Raval, Prashant Arya, Monika Jain, Tarun Sosa, Preya Trivedi & Vikram Hiren Raval

  2. Department of Chemistry, School of Sciences, Gujarat University, Ahmedabad, 380009, India

    Ranjitsinh Dabhi

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Contributions

SYR, PSA, TS, MJ, and PT practically performed the experiments, collected the data, and analyzed the data. RD performed the analytical characterization, while PSA prepared the draft of the manuscript. The designing, language correction, and data curation were done by SYR. The overall idea of the work, experiment, and manuscript was done by VHR.

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Correspondence to Vikram Hiren Raval.

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Highlights

• Sugarcane bagasse was found to be a promising and inexpensive substrate for biosynthesis of β-carotene by Nesterenkonia sp. reported for the first time.

• Easy purification and characterization of β-carotene using TLC, HPTLC, FTIR, and MS.

• Intense color makes it a good alternative for diverse biotechnological applications.

• Tried and tested as non-phytotoxic, antimicrobial, antioxidant, textile dyeing, food additives, cosmetics, and candles.

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Raval, S.Y., Arya, P., Jain, M. et al. Sustainable biosynthesis of β-carotene utilizing sugarcane bagasse: depiction and biotechnological implications. Biomass Conv. Bioref. (2024). https://doi.org/10.1007/s13399-024-05815-8

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  • DOI: https://doi.org/10.1007/s13399-024-05815-8

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